A5046821623- Circadian rhythm and melatonin
- Genetics, Aging, and Longevity in Model Organisms
- Luminescence and Fluorescent Materials
- Neuroendocrine regulation and behavior
- Molecular Sensors and Ion Detection
- Polymer Surface Interaction Studies
- Polydiacetylene-based materials and applications
- Nanoplatforms for cancer theranostics
- Rabbits: Nutrition, Reproduction, Health
- Photoreceptor and optogenetics research
- Advanced Fluorescence Microscopy Techniques
University of Miami
2017-2022
A photochemical strategy to encode fluorescence signals in vivo with spatial control was designed around the unique properties of a photoactivatable borondipyrromethene (BODIPY). The photoinduced disconnection two oxazines, flanking single BODIPY, consecutive steps produces mixture three emissive molecules resolved inside polymer beads. relative amounts and emission intensities fluorophores can be regulated precisely each bead by adjusting dose activating photons mark individual particles...
Seven macromolecular constructs incorporating multiple borondipyrromethene (BODIPY) fluorophores along a common poly(methacrylate) backbone with decyl and oligo(ethylene glycol) side chains were synthesized. The hydrophilic components impose solubility in aqueous environment on the overall assembly. hydrophobic effectively insulate from each other to prevent detrimental interchromophoric interactions preserve their photophysical properties. As result, brightness of these multicomponent...
It has become increasingly clear that neural circuit activity in behaving animals differs substantially from seen anesthetized or immobilized animals. Highly sensitive, genetically encoded fluorescent reporters of Ca2+ have revolutionized the recording cell and synaptic using non-invasive optical approaches When combined with genetic optogenetic techniques, molecular mechanisms modulate during different behavior states can be identified. Here we describe methods for ratiometric imaging...
Abstract Activated Gαq signals through phospholipase-Cβ and Trio, a Rho GTPase exchange factor (RhoGEF), but how these distinct effector pathways promote cellular responses to neurotransmitters like serotonin remains poorly understood. We used the egg-laying behavior circuit of Caenorhabditis elegans determine whether Trio mediate signaling independent or related biochemical pathways. Our genetic rescue experiments suggest that functions in neurons while both postsynaptic vulval muscles....
It has become increasingly clear that neural circuit activity in behaving animals differs substantially from seen anesthetized or immobilized animals. Highly sensitive, genetically encoded fluorescent reporters of Ca2+ have revolutionized the recording cell and synaptic using non-invasive optical approaches When combined with genetic optogenetic techniques, molecular mechanisms modulate during different behavior states can be identified. Here we describe methods for ratiometric imaging...
Amphiphilic macromolecules with multiple borondipyrromethene (BODIPY) chromophores appended to a common poly(methacrylate) backbone were synthesized by the random co-polymerization of appropriate methacrylate monomers. The resulting polymers incorporate also hydrophilic oligo(ethylene glycol) and hydrophobic decyl side chains designed impose aqueous solubility insulate chromophoric components from each other respectively. presence translates into significant enhancement in molar absorption...
Abstract Activated Gα q signals through Phospholipase-Cβ (PLCβ) and Trio, a Rho GTPase exchange factor (RhoGEF), but how these distinct effector pathways promote cellular responses to neurotransmitters like serotonin remains poorly understood. We used the egg-laying behavior circuit of C. elegans determine whether PLCβ Trio mediate signaling independent or related biochemical pathways. Our genetic rescue experiments suggest that functions in neurons while RhoGEF both postsynaptic vulval...