A5101654292- Traditional Chinese Medicine Analysis
- Phytochemistry and Biological Activities
- Fungal Biology and Applications
- Natural product bioactivities and synthesis
- Traditional Chinese Medicine Studies
- Chromatography in Natural Products
- Ginseng Biological Effects and Applications
- Plant-based Medicinal Research
- Pharmacological Effects of Natural Compounds
- Medicinal Plant Pharmacodynamics Research
- Nephrotoxicity and Medicinal Plants
- Liver Disease Diagnosis and Treatment
- Drug-Induced Hepatotoxicity and Protection
- Plant and fungal interactions
- Plant-derived Lignans Synthesis and Bioactivity
- Phytochemistry and Bioactive Compounds
- Analytical Chemistry and Chromatography
- Healthcare and Venom Research
- Heavy Metals in Plants
- Phytochemical Studies and Bioactivities
- Food Quality and Safety Studies
- Medical Research and Treatments
- Plant biochemistry and biosynthesis
- Liver physiology and pathology
- Sperm and Testicular Function
Beijing University of Chinese Medicine
2014-2024
Shenyang Pharmaceutical University
2015-2017
National Institutes for Food and Drug Control
2005-2014
United States Food and Drug Administration
2013
Northwest University
2009-2011
Université de Bourgogne
1996
Abstract Alternative medicines are commonly used for the disease prevention and treatment worldwide. Aristolochic acid (AAI) nephropathy (AAN) is a common rapidly progressive interstitial caused by ingestion of Aristolochia herbal medications. Available data on pathophysiology molecular mechanisms AAN limited were explored here. SD rats randomized to control groups. group was treated with AAI oral gavage 12 weeks observed additional weeks. Kidneys processed histological evaluation, Western...
Abstract Chronic kidney disease (CKD) is a major public health problem worldwide. Rhubarb has been shown to have nephroprotective and anti-fibrotic activities in patients with CKD. However, bioactive fractions biochemical mechanism of properties rhubarb remain unclear. Here we applied ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry together univariate multivariate statistical analyses investigate the urinary metabolite profile rats adenine-induced CKD...
Rheumatoid arthritis (RA) is a common chronic immune disease. Berberine, as its main active ingredient, was also contained in variety of medicinal plants such Berberaceae, Buttercup, and Rutaceae, which are widely used digestive system diseases traditional Chinese medicine with anti-inflammatory antibacterial effects. The aims this article were to explore the therapeutic effect mechanism berberine on rheumatoid arthritis.Cell Counting Kit-8 evaluate proliferation RA fibroblast-like...
The steroids ergone (1), (22E, 24R)-ergosta-7,22-dien-3β-ol (2), 5α,8α-epidioxy-(22E,24R) ‐ergosta-6,22-dien-3β-ol (3), ergosta-6,22-dien-3β,5α,6β-triol (4), and polyporusterone B (5) were isolated from Polyporus umbellatus by bioassay-guided approach. They showed potent anticancer activity against HepG2 cells. Ergone displayed remarkable HepG2, Hep-2, Hela cancer cells, of which cells the most sensitive. Furthermore, cytotoxic effects on normal human (HUVEC) smaller than results that had...
Abstract Objectives Aristolochic acid (AA) nephropathy, first reported as Chinese herbs is a rapidly progressive tubulointerstitial nephropathy that results in severe anemia, interstitial fibrosis and end-stage renal disease. Tubulointerstitial injury was studied rat model of AA to determine whether ergosta-4,6,8(14),22-tetraen-3-one (ergone) treatment prevents early rats with aristolochic I-induced nephropathy. Methods Early via induced by administration I (AAI) solution intragastrically...
MicroRNAs (miRNAs) are a novel class of powerful, endogenous regulators gene expression. This study was designed to ascertain if miR-30a is involved in the cardioprotective actions salvianolic acid B (Sal B) against myocardial ischemia–reperfusion (I–R) injury through suppression autophagy. Murine cells that had undergone primary culture were induced by I–R and incubated with Sal (25, 50, 100 μM) presence mimic or inhibitor. Expression miR-30a, beclin-1, LC3-II p-Akt protein, cell viability,...
Wuzi-Yanzong-Wan (WZYZW), a classical traditional Chinese medicine (TCM) prescription containing Fructus Lych, Semen Cuscutae (fried), Rubi, Schisandrae chinensis (steamed) and Plantaginis (fried with salt), is widely used to treat impotence, sterility, spermatorrhea, premature ejaculation, lumbago post-micturation dribble. However, the chemical profile of WZYZW has not been established yet. In this work, rapid sensitive method for systematically screening identifying constituents in both...
This study aimed to investigate if ellagic acid has beneficial effects on cognitive deficits in middle-aged overweight individuals and propose a possible mechanism. A total of 150 male participants, including 76 normal-weight 74 men, aged between 45 55 years, were recruited for this study. Both participants administered either 50 mg or placebo cellulose daily 12 weeks. Blood lipids, peripheral brain-derived neurotrophic factor (BDNF), saliva cortisol assessed the last day procedure induced...
Background: Yiqi Huoxue Decoction (YQHXD) is a traditional Chinese medicine that promotes blood circulation, removes stasis, facilitates diuresis, and alleviates edema. It composed of 10 herbal medicines has extensive application in treating nephrotic syndrome (NS). However, the active components potential mechanism YQHXD for NS remain unclear. Methods: We set up sensitive rapid method based on Ultra-High Performance Liquid Chromatograph-Mass (UPLC-MS) to identify compounds constituents...
Polyphyllin I (PPI) and polyphyllin II (PII) are the main active substances in Paris polyphylla. However, liver toxicity of these compounds has impeded their clinical application potential hepatotoxicity mechanisms remain to be elucidated. In this work, we found that PPI PII exposure could induce significant human cell line L-02 zebrafish a dose-dependent manner. The results proteomic analysis cells transcriptome indicated was associated with cholesterol biosynthetic pathway disorders, which...
Salvianolic acid Y (TSL 1), a new phenolic with the same planar structure as salvianolic B, was isolated from Salvia officinalis. The structural elucidation and stereochemistry determination were achieved by spectroscopic chemical methods, including 1D, 2D-NMR (1H-1H COSY, HMQC HMBC) circular dichroism (CD) experiments. biosynthesis pathway of B 1) proposed based on analysis. protection PC12 cells injury induced H2O2 assessed in vitro using cell viability assay. protected 54.2%, which...
A set of simple TLC and HPLC-PDA-MS methods were developed for detection counterfeit herbal dietary supplements by the qualitative quantitative analysis eight phosphodiesterase type 5 inhibitors (PDE5-Is), sildenafil, hongdenafil, homosildenafil, hydroxyhomosildenafil, vardenafil, pseudovardenafil, tadalafil, aminotadalafil. The analytical approach is rapid screening with reliable HPLC-PDA-MS. Good linearity (r 2 > 0.999 over concentration range 8.1 to 211.2 μg/ml), sensitivity (limits...
Background: Rhaponticin (Rheum L.) demonstrates a variety of pharmacological activities, including antitumor, antithrombotic and antioxidant effect. However, there is no information describing the pharmacokinetics, bioavailability metabolism rhaponticin after intravenous administration. Results: UHPLC–Q-TOF/MS UHPLC–multistage tandem MS methods were developed for in rats. The metabolite rhaponticin, rhapontigenin, potent inhibitor cytochrome P450, was confirmed by MS. plasma profile...
An accurate and sensitive high-performance liquid chromatography method coupled with ultralviolet detection precolumn derivatization was developed for the simultaneous quantification of major bile acids in Artificial Calculus bovis, including cholic acid, hyodeoxycholic chenodeoxycholic deoxycholic acid. The extraction, derivatization, chromatographic separation, parameters were fully optimized. samples extracted methanol by ultrasonic extraction. Then, 2-bromine-4'-nitroacetophenone...
An HPLC-UV-MS method for the analysis of aristolochic acids A, B, C and D, 7-OH-aristolochic acid aristolic in a number plant materials their commercial products has been developed. HPLC with photodiode array detection electrospray ionisation-MS selected ion monitoring mode allowed identification target compounds increased selectivity complex analyses such as those associated multi-botanical preparations. The presented was used to analyse 10 samples six that possibly contained acids....