A5024095283- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Breast Cancer Treatment Studies
- Breast Lesions and Carcinomas
- Cancer-related Molecular Pathways
- Cancer and Skin Lesions
- Genetic factors in colorectal cancer
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Gene expression and cancer classification
- Bone health and treatments
- Advanced Breast Cancer Therapies
- Metastasis and carcinoma case studies
- Bone Metabolism and Diseases
- Epigenetics and DNA Methylation
- Cytokine Signaling Pathways and Interactions
- Estrogen and related hormone effects
- Soft tissue tumor case studies
- HER2/EGFR in Cancer Research
- Colorectal Cancer Treatments and Studies
- Molecular Biology Techniques and Applications
- Neuroendocrine Tumor Research Advances
- Ubiquitin and proteasome pathways
- Circular RNAs in diseases
- Cancer Diagnosis and Treatment
Thomas Jefferson University Hospital
2012-2025
University of North Carolina at Chapel Hill
2006-2023
Thomas Jefferson University
2012-2023
ARUP Institute for Clinical and Experimental Pathology
2009-2023
Baptist Health South Florida
2020
Baptist Hospital of Miami
2019-2020
Sidney Kimmel Cancer Center
2002-2018
Jefferson College
1997-2018
Jefferson University Hospitals
2016-2018
Nantong University
2018
Purpose To improve on current standards for breast cancer prognosis and prediction of chemotherapy benefit by developing a risk model that incorporates the gene expression–based “intrinsic” subtypes luminal A, B, HER2-enriched, basal-like. Methods A 50-gene subtype predictor was developed using microarray quantitative reverse transcriptase polymerase chain reaction data from 189 prototype samples. Test sets 761 patients (no systemic therapy) were evaluated prognosis, 133 pathologic complete...
Abstract MicroRNAs (miRNAs) are a class of small noncoding RNAs that control gene expression by targeting mRNAs and triggering either translation repression or RNA degradation. Their aberrant may be involved in human diseases, including cancer. Indeed, miRNA has been previously found chronic lymphocytic leukemias, where signatures were associated with specific clinicobiological features. Here, we show that, compared normal breast tissue, miRNAs also aberrantly expressed The overall could...
Abstract Background Validation of a novel gene expression signature in independent data sets is critical step the development clinically useful test for cancer patient risk-stratification. However, validation often unconvincing because size set typically small. To overcome this problem we used publicly available breast and approach to fusion, order validate new tumor intrinsic list. Results A 105-tumor training containing 26 sample pairs was derive This list contained 1300 genes...
Abstract Background Although numerous mouse models of breast carcinomas have been developed, we do not know the extent to which any faithfully represent clinically significant human phenotypes. To address this need, characterized mammary tumor gene expression profiles from 13 different murine using DNA microarrays and compared resulting data those tumors. Results Unsupervised hierarchical clustering analysis showed that six (TgWAP- Myc , TgMMTV- Neu PyMT TgWAP- Int3 Tag TgC3(1)- ) yielded...
Metaplastic breast cancers (MBC) are aggressive, chemoresistant tumors characterized by lineage plasticity. To advance understanding of their pathogenesis and relatedness to other cancer subtypes, 28 MBCs were compared with common using comparative genomic hybridization, transcriptional profiling, reverse-phase protein arrays sequencing for mutations. showed unique DNA copy number aberrations cancers. PIK3CA mutations detected in 9 19 (47.4%) versus 80 232 hormone receptor-positive (34.5%; P...
Abstract Small non‐coding microRNAs (miRNAs) contribute to cancer development and progression, are differentially expressed in normal tissues cancers. However, the specific role of miRNAs metastatic process is still unknown. To seek a miRNA expression signature characterizing phenotype solid tumours, we performed microarray analysis on 43 paired primary tumours (ten colon, ten bladder, 13 breast, lung cancers) one their related lymph nodes. We identified comprising 15 overexpressed 17...
Significance Although transfer RNAs (tRNAs) are best known as adapter molecules essential for translation, recent biochemical and computational evidence has led to a previously unexpected conceptual consensus that tRNAs not always end products but can further serve source of small functional RNAs. Here we report novel type tRNA-derived RNA, termed SHOT-RNAs, specifically abundantly expressed in sex hormone-dependent breast prostate cancers. SHOT-RNAs produced from aminoacylated mature by...
To improve on current standards for breast cancer prognosis and prediction of chemotherapy benefit by developing a risk model that incorporates the gene expression-based "intrinsic" subtypes luminal A, B, HER2-enriched, basal-like.
Triple-negative breast cancers (TNBC) frequently inactivate p53, increasing their aggressiveness and therapy resistance. We identified an unexpected protein vulnerability in p53-inactivated TNBC designed a new PROteolysis TArgeting Chimera (PROTAC) to target it. Our PROTAC selectively targets MDM2 for proteasome-mediated degradation with high-affinity binding VHL recruitment. loss p53 mutant/deleted cells two-dimensional/three-dimensional culture patient explants, including relapsed tumors,...
Defective DNA mismatch repair in human tumors leads to genome-wide instability of microsatellite repeats and a molecular phenotype referred as (MSI). MSI has been reported variety cancers is consistent feature from patients with hereditary non-polyposis colorectal cancer. Approximately 20% the uterine endometrium, fifth most common cancer women world-wide, exhibit MSI. Although frequency higher endometrial than any other malignancy, genetic basis these remained elusive. We investigated role...
<h3>AIM</h3> Colorectal cancer has been described in association with hyperplastic polyposis but the mechanism underlying this observation is unknown. The aim of study was to characterise foci dysplasia developing polyps subjects on basis DNA microsatellite status and expression mismatch repair proteins hMLH1, hMSH2, hMSH6. <h3>MATERIALS AND METHODS</h3> material derived from four patients between one six synchronous colorectal cancers. Normal (four), (13), dysplastic malignant (11) samples...
Abstract Introduction Predicting the clinical course of breast cancer is often difficult because it a diverse disease comprised many biological subtypes. Gene expression profiling by microarray analysis has identified signatures that are important for prognosis and treatment. In current article, we use real-time quantitative reverse-transcription (qRT)-PCR assay to risk-stratify cancers based on 'intrinsic' subtypes proliferation. Methods sets were selected from data assess proliferation...
We used comparative genomic hybridization (CGH) to identify recurrent chromosomal imbalances in tumor DNA from 25 malignant ovarian carcinomas and two tumors of low potential (LMP). Many the carcinoma specimens displayed numerous imbalances. The most common sites copy number increases, order frequency, were 8q24.1, 20q13.2-qter, 3q26.3-qter, 1q32, 20p, 9p21-pter, 12p. amplification was identified 12 (48%). frequent 8q24.1-24.2, 3q26.3, 20q13.2-qter. Other included 7q36, 17q25, 19q13.1-13.2....
// Aristeidis G. Telonis 1 , Phillipe Loher Shozo Honda Yi Jing Juan Palazzo 2 Yohei Kirino and Isidore Rigoutsos Computational Medicine Center, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA Department of Pathology Anatomy Cell Biology, Correspondence to: Rigoutsos, email: Keywords : tRNA fragment, human genome, nuclear tRNA, mitochondrial Argonaute Received June 10, 2015 Accepted 20, Published July 06, Abstract We analyzed transcriptomic data from 452...
Adenomyoepithelioma of the breast is a rare tumor characterized by epithelial-myoepithelial differentiation, whose genetic underpinning largely unknown. Here we show through whole-exome and targeted massively parallel sequencing analysis that whilst estrogen receptor (ER)-positive adenomyoepitheliomas display PIK3CA or AKT1 activating mutations, ER-negative harbor highly recurrent codon Q61 HRAS hotspot which co-occur with PIK3R1 mutations. In two- three-dimensional cell culture models,...
Abstract Granular cell tumors (GCTs) are rare that can arise in multiple anatomical locations, and characterized by abundant intracytoplasmic granules. The genetic drivers of GCTs currently unknown. Here, we apply whole-exome sequencing targeted analysis to reveal mutually exclusive, clonal, inactivating somatic mutations the endosomal pH regulators ATP6AP1 or ATP6AP2 72% GCTs. Silencing these genes vitro results impaired vesicle acidification, redistribution compartments, accumulation...
Metaplastic breast carcinoma (MBC) is a rare subtype of less responsive to conventional chemotherapy than ductal carcinoma. In molecular terms, MBCs usually cluster with triple-negative cancers (TNBCs), but have worse prognosis TNBCs. Studies investigating for specific biomarkers therapy response are and limited by the methodological approaches. The aim present study was characterise on level test programmed death-ligand 1 (PD-L1) biomarker expression in future therapeutic interventions.We...