A5012396048- Ethics in Clinical Research
- Biomedical Ethics and Regulation
- Virus-based gene therapy research
- Telemedicine and Telehealth Implementation
- Herpesvirus Infections and Treatments
- Health Systems, Economic Evaluations, Quality of Life
- interferon and immune responses
- Poxvirus research and outbreaks
- Artificial Intelligence in Healthcare and Education
- Planarian Biology and Electrostimulation
- Immune Response and Inflammation
- Chronic Disease Management Strategies
- Biomedical and Engineering Education
- Healthcare Systems and Technology
- Pelvic floor disorders treatments
- Healthcare Systems and Public Health
- Mycobacterium research and diagnosis
- COVID-19 and healthcare impacts
- Wound Healing and Treatments
- Immune Cell Function and Interaction
- Public Health Policies and Education
- Privacy, Security, and Data Protection
- Protein Kinase Regulation and GTPase Signaling
- Myofascial pain diagnosis and treatment
- Cell death mechanisms and regulation
Universidade Nova de Lisboa
2013-2025
University of Lisbon
2013-2025
National School of Public Health
2024
Escola Nacional de Saúde Pública
2013-2022
The Francis Crick Institute
2017
Institute for Cross-Disciplinary Physics and Complex Systems
2014
MRC Laboratory for Molecular Cell Biology
2009
Universidade do Porto
2008
The Honourable Society of Lincoln's Inn
2006-2007
Cancer Research UK
2006-2007
Epithelial wound healing relies on tissue movements and cell shape changes. Our work shows that, immediately after wounding, there was a dramatic cytoskeleton remodeling consisting of pulse actomyosin filaments that assembled in cells around the edge flowed from to toward margin wound. We show this flow regulated by Diaphanous ROCK it elicited wave apical constriction culminated formation leading cable, structure is essential for closure. Calcium signaling played an important role process,...
Artificial Intelligence (AI) is increasingly influential across various sectors, including healthcare, with the potential to revolutionize clinical practice. However, risks associated AI adoption in medicine have also been identified. Despite general understanding that will impact studies assess perceptions of medical doctors about use are still scarce. We set out survey licensed practice Portugal impact, advantages, and disadvantages designed an observational, descriptive, cross-sectional...
RhoA signaling plays a critical role in many cellular processes, including cell migration. Here we show that the vaccinia F11L protein interacts directly with RhoA, inhibiting its by blocking interaction downstream effectors Rho-associated kinase (ROCK) and mDia. RNA interference-mediated depletion of during infection resulted an absence vaccinia-induced motility inhibition viral morphogenesis. Disruption binding site F11L, which resembles ROCK, led to identical phenotype. Thus, is required...
The cortical actin cytoskeleton beneath the plasma membrane represents a physical barrier that vaccinia virus has to overcome during its exit from an infected cell. Previous observations using overexpression and pharmacological approaches suggest enhances release by modulating inhibiting RhoA signalling viral protein F11. We have now examined role of F11 ability interact with inhibit downstream in spread infection both vitro vivo. Live cell imaging over 48 hours reveals loss or bind...
RhoA-mediated regulation of myosin-II activity in the actin cortex controls ability cells to contract and bleb during a variety cellular processes, including cell migration division. Cell contraction blebbing also frequently occur as part cytopathic effect seen many different viral infections. We now demonstrate that vaccinia virus protein F11, which localizes plasma membrane, is required for ROCK-mediated from 2 hr post infection. Curiously, F11-induced dependent on RhoC not RhoA signaling...
High-resolution anorectal manometry (HR-ARM) is the gold standard for functional disorders' evaluation, despite being limited by its accessibility and complex data analysis. The London Protocol Classification were developed to standardize motility patterns classification. This proof-of-concept study aims develop validate an artificial intelligence model identification differentiation of disorders anal tone contractility in HR-ARM. A dataset 701 HR-ARM exams from a tertiary center, classified...
Toll-like receptor (TLR) 2 plays an important role in the immune response to mycobacterial infections, being required for optimal immunity against certain virulent Mycobacterium avium strains. Here we analyzed of TLR2 intra-macrophagic growth M. avium, using macrophages from TLR2-deficient mice. We found that engagement TLR2/TLR6 and/or TLR2/TLR1 receptors induced bacteriostasis inside bone marrow-derived a MyD88-dependent way. Additionally, lipoproteins cell envelope with molecular mass...
The role of the Toll-like receptor (TLR)-2 in generation protective immunity to Mycobacterium avium was evaluated using gene-disrupted mice. TLR-2-/- mice were more susceptible than wild-type C57Bl/6 M. strains that able proliferate vivo before development and mycobacteriostasis. In contrast, elimination non-virulent not affected by mutation. interferon-gamma (IFN-gamma)-producing T cells expression interleukin-12 p40 gene reduced TLR-2-deficient as compared early during infection with...
Although widely analyzed by authors and theoretically valued the public, right to health data confidentiality seems be more of an academic figure than a real protected right. This happens due intrinsic problems with practical enforcement some patient's rights, but is getting notorious in contemporary society. article describes rights privacy their classical contours, focusing on areas consensus controversy analyzing recent transformations society that are causing crisis these same rights. We...
This article overviews the important ethical and legal challenges of different steps personalized medicine journey such as research, prevention, diagnosis treatment by discussing paradigmatic examples including biobanks, genetic tests gene therapy. Scientific progress in area genetics, completion Human Genome Project ability to sequence genomes for competitive prices have offered promise revolutionizing healthcare raised classical paradigms biomedical law ethics fields. Issues informed...
Introduction: The COVID-19 pandemic has accelerated the adoption of telemedicine as a means reducing face-to-face contact and protecting professionals patients. In Portugal, number hospital consultations significantly increased. However, rapid implementation also led to disparities in access these services, resulting inequalities healthcare delivery. aim this study was identify main challenges accessing medical specialty through Portugal during pandemic. Additionally, aimed establish...
Approximately 10% of patients experience symptoms Post COVID-19 Condition (PCC) after a SARS-CoV-2 infection. Akin acute COVID-19, PCC may impact multitude organs and systems, such as the cardiovascular, respiratory, musculoskeletal, neurological systems. The frequency associated risk factors are still unclear among both community hospital settings in individuals with history COVID-19. LOCUS study was designed to clarify PCC’s burden factors. is multi-component that encompasses three...