A5041756639- Veterinary Oncology Research
- Infectious Diseases and Mycology
- Virus-based gene therapy research
- Salivary Gland Tumors Diagnosis and Treatment
- Oral and Maxillofacial Pathology
- Sarcoma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Hedgehog Signaling Pathway Studies
- Tumors and Oncological Cases
- Cancer Diagnosis and Treatment
- Cancer Research and Treatments
- HER2/EGFR in Cancer Research
- Skin and Cellular Biology Research
- Retinal Development and Disorders
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- Cancer and Skin Lesions
- Microbial infections and disease research
- Genetic and Kidney Cyst Diseases
- Genetic Syndromes and Imprinting
- Angiogenesis and VEGF in Cancer
- Fibroblast Growth Factor Research
- Gastrointestinal Tumor Research and Treatment
- Soft tissue tumor case studies
- Photoreceptor and optogenetics research
University of Bologna
2015-2024
Evotec (United Kingdom)
2023
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2023
Evotec (United States)
2023
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
2022
Ospedale San Giacomo Apostolo
2015
During the summer of 2013 seven Italian Tyrolean Grey calves were born with abnormally short limbs. Detailed clinical and pathological examination revealed similarities to chondrodysplastic dwarfism. Pedigree analysis showed a common founder, assuming autosomal monogenic recessive transmission defective allele. A positional cloning approach combining genome wide association homozygosity mapping identified single 1.6 Mb genomic region on BTA 6 that was associated disease. Whole re-sequencing...
Using immunohistochemistry, 170 canine mammary carcinomas were evaluated for p53, ER (estrogen receptor), and Ki67. Of the tumors, 89 grade I (52.3%), 36 II (21.2%), 45 III (26.4%). Eight cases (0.5%) positive p53 69/170 (40.5%) ER. Ki67 values 24 ± 18% (mean SD). a cutoff value of 33.3% Ki67-positive neoplastic nuclei, 38/159 (23.8%) classified as high proliferative 121/159 (76.2%) low proliferative. p53-positive had significantly higher expression histological grade. was not correlated...
Doxorubicin (DOX) is widely used in both human and veterinary oncology although the onset of multidrug resistance (MDR) neoplastic cells often leads to chemotherapy failure. Better understanding cellular mechanisms that circumvent efficacy paramount. The aim this study was investigate response two canine mammary tumour cell lines, CIPp from a primary CIPm, its lymph node metastasis, exposure EC50(20h) DOX at 12, 24 48 h treatment. We assessed uptake subcellular distribution DOX, expression...
Canine cutaneous round cell tumors (RCT) represent one of the routine diagnostic challenges for veterinary pathologists. Computer-aided approaches are developed to overcome these restrictions and increase accuracy consistency diagnosis. These systems also high benefit reducing errors when a large number cases screened daily. In this study we describe ARCTA (Automated Round Cell Tumors Assessment), fully automated algorithm RCT classification mast grading in canine histopathological images....
This study aimed to evaluate the relationship between molecular phenotype of primary mammary tumor and its related lymph node metastasis in dog develop prognostic-predictive models targeted therapeutic options.Twenty samples their metastases were selected stained by immunohistochemistry with anti-estrogen receptor (ER), -progesterone (PR), -human epidermal growth factor 2 (c-erbB-2), -cytokeratin 5/6 (CK 5/6), 14 (CK14), 19 19) -protein 63 (p63) antibodies. Four phenotypes (luminal A,...
Human epidermal growth factor receptor 2 (HER2) is a tyrosine kinase that promotes tumor cell and implicated in the pathogenesis of human breast cancer. The role HER2 canine mammary carcinomas (CMCs) not clear. Therefore, this study aimed to examine protein expression cytogenetic changes their correlation with other clinical-pathological parameters CMC. We retrospectively selected 112 CMCs. HER2, ER, Ki67 were assessed by immunohistochemistry. antibody validation was investigated immunoblot...
HER2 overexpression has been reported in various human and canine tumours. The aim of this study is to investigate the expression protein different histotypes carcinomas order identify potential tumours that could benefit from HER2-targeted therapy. Eighty-two (82) (squamous cell, gastro-intestinal, rectal, pulmonary, prostatic, urothelial, ovarian) paraffin-embedded samp les were immunohistochemically evaluated. degree was scored based on ASCO/CAP 2018 guidelines. Intestinal those with...
Human epidermal growth factor receptor 2 (HER2) is a tyrosine kinase overexpressed in subset of breast cancer due to HER2 gene amplification. protein expressed feline mammary carcinomas, but little known about its cytogenetic alterations. The aim this study was evaluate amplification status and correlation with expression carcinomas. Feline carcinomas were retrospectively selected immunohistochemically (IHC) evaluated for expression. All the IHC-positive (3+) equivocal (2+) cases negative...
P-glycoprotein (P-gp/ABCB1) and breast cancer resistance protein (BCRP/ABCG2) expression are frequently related to multidrug (MDR) in neoplastic cells. Canine inflammatory grade III noninflammatory mammary carcinomas (IMC non-IMC) aggressive tumors that could benefit from chemotherapy. This study describes the immunohistochemical detection of P-gp BCRP 20 IMCs 18 non-IMCs dogs had not received Our aim was determine if “inflammatory” phenotype, establish a basis for future studies analyzing...
Ellis-van Creveld (EvC) syndrome is a human autosomal recessive disorder caused by mutation in either the EVC or EVC2 gene, and presents with short limbs, polydactyly, ectodermal heart defects. The aim of this study was to understand pathologic basis which deletions gene lead chondrodysplastic dwarfism describe morphologic, immunohistochemical, molecular hallmarks EvC cattle. Five Grey Alpine calves, known were autopsied. Immunohistochemistry performed on bone using antibodies collagen II,...
In human medicine, p53 immunohistochemistry (IHC) is a common method that used for the identification of tumors with TP53 mutations. veterinary several studies have performed IHC in canine tumors, but it not known how well actually predicts mutation. The aim this study was to estimate accuracy (clone PAb240) using lab-developed NGS panel analyze mutations subset malignant dogs. A total 176 were analyzed and then 41 subjected analysis; among them, 15 positive 26 negative, 16 out (39%) found...
Methods This study aimed to examine the pathological impact of Porcine Circovirus type 2 (PCV2) and Reproductive Respiratory Syndrome Virus (PRRSV) through histological immunohistochemical analysis 79 cases Disease Complex (PRDC) collected from 22 farms in Northern Italy. Lung tissue several lymphoid organ samples were deployed associate PCV2-positive stain with Circovirus-associated Diseases (PCVD). Results The most common lung lesion observed was interstitial pneumonia, alone or combined...
Abstract Canine liposarcoma is an uncommon tumor that shares morphological similarities with its human counterpart. In dogs, the genetic features of this are unknown and, based on immunohistochemical studies, amplification gene MDM2 and mutation TP53 suspected. study 51 cases primary liposarcomas were immunohistochemically stained for p53 subjected to fluorescent in situ hybridization next-generation sequencing detect mutations, respectively. expressed 21 6 cases, mutations identified 10 15...
Skeletal dysplasia encompasses a heterogeneous group of genetic disorders characterized by an abnormal development bones, joints, and cartilage. Two Chianina half-sibling calves from consanguineous mating with congenital skeletal malformations cardiac abnormalities were identified.
Choroid plexus tumors (CPT) are intraventricular neoplasms accounting for 10% of all primary central nervous system in dogs. They frequently classified according to the human WHO classification into choroid papilloma (CPP, grade I), atypical CPP (aCPP, II), and carcinoma (CPC, III). Histological features observed canine CPT such as increased vascular density (IVD) glomeruloid microvascular proliferation (GMVP) not part classification. This multi-centric study aimed investigate...
A 9-month old male Jack Russell Terrier started showing paraparesis of the hindlimbs after a walk. Hospitalized, dog went into cardiac arrest, and later died. Necroscopic examination revealed severe thickness diaphragm, esophagus, base tongue, leading to diagnosis muscular dystrophy. The histology confirmed marked size variation, regeneration, fibrosis replacement skeletal muscle fibers. Immunohistochemistry demonstrated absence dystrophin confirming diagnosis. Transmission electron...
Although the influence of copper ([Cu]) on chronic hepatitis (CH) has been widely studied in dogs, little information is available about accumulation other metals.We assessed concentration lead ([Pb]) livers dogs with CH or without abnormal hepatic [Cu] to establish if any association existed between [Pb] and either hematologic biochemical variables, fibrosis, necrosis inflammation liver histology.Thirty-four that had determined.Retrospective review medical records [Pb]. Chronic was defined...
Canine smooth muscle tumors (SMTs) commonly develop in the alimentary and female genital tracts less frequently soft tissue. The definition of histological criteria malignancy is detailed for SMTs dogs than humans. This study evaluated clinicopathologic features canine compared veterinary human medical malignancy. A total 105 were histologically classified according to both criteria. Ki67 labeling index was assessed all SMTs. Estrogen receptor (ER) progesterone (PR) expression tissue...