A5033600988- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Single-cell and spatial transcriptomics
- Fungal and yeast genetics research
- Enzyme Catalysis and Immobilization
- Bioinformatics and Genomic Networks
- Fungal Biology and Applications
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
- Hedgehog Signaling Pathway Studies
- Biopolymer Synthesis and Applications
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Muscle Physiology and Disorders
- Protein Kinase Regulation and GTPase Signaling
- Autophagy in Disease and Therapy
- Animal Genetics and Reproduction
- Fermentation and Sensory Analysis
- Enzyme Production and Characterization
- Gene Regulatory Network Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Reproductive Biology
- Neural dynamics and brain function
- Microbial metabolism and enzyme function
University of North Carolina at Chapel Hill
2020-2025
Nanjing Forestry University
2024-2025
Northeast Agricultural University
2012-2025
Indiana University – Purdue University Indianapolis
2025
Zhejiang University
2021-2024
Peking University
2014-2024
Peking University Third Hospital
2024
UNC Lineberger Comprehensive Cancer Center
2024
Guizhou University
2022-2024
Zhongnan Hospital of Wuhan University
2024
Diverse forms of unwanted signal variations in mass spectrometry-based metabolomics data adversely affect the accuracies metabolic profiling. A variety normalization methods have been developed for addressing this problem. However, their performances vary greatly and depend heavily on nature studied data. Moreover, given complexity actual data, it is not feasible to assess performance by single criterion. We therefore NOREVA enable evaluation various from multiple perspectives. integrated...
Abstract Purpose This article summarizes natural products that target the MAPK-signaling pathway in cancer therapy. The classification, chemical structures, and anti-cancer mechanisms of these are elucidated, comprehensive information is provided on their potential use Methods Using PubMed database, we searched for keywords, including “tumor”, “cancer”, “natural product”, “phytochemistry”, “plant components”, “MAPK-signaling pathway”. We also screened compounds with well-defined structures...
Abstract The phytohormone gibberellic acid (GA) regulates diverse aspects of plant growth and development. GA responses are triggered by the degradation DELLA proteins, which function as repressors in signaling pathways. Recent studies Arabidopsis thaliana rice (Oryza sativa) have implied that proteins occurred via ubiquitin-proteasome system. Here, we developed an cell-free system to recapitulate protein vitro. Using this system, documented Lys-29 ubiquitin is major site for chain formation...
In untargeted metabolomics analysis, several factors (e.g., unwanted experimental &biological variations and technical errors) may hamper the identification of differential metabolic features, which requires data-driven normalization approaches before feature selection. So far, ≥16 methods have been widely applied for processing LC/MS based data. However, performance sample size dependence those not yet exhaustively compared no online tool comparatively comprehensively evaluating all 16 has...
In order to address the dire need for new antibiotics treat specific strains of drug resistant Gram-negative bacterial infections, a mixed ligand analog natural Acinetobacter baumannii selective siderophore, fimsbactin, was coupled daptomycin, Gram-positive only antibiotic. The resulting conjugate 11 has potent activity against multidrug A. both in vitro and vivo. study also indicates that conjugation siderophores "drugs" are much larger than siderophore (iron transport agent) itself...
Wood formation is controlled by transcriptional regulatory networks (TRNs) involving homeostasis determined combinations of transcription factor (TF)-DNA and TF-TF interactions. Functions interactions in wood are still the early stages identification. PtrMYB074 a woody dicot-specific TF TRN for Populus trichocarpa. Here, using yeast two-hybrid bimolecular fluorescence complementation, we conducted genome-wide screening interactors identified 54 PtrMYB074-TF pairs. Of these pairs, 53 novel....
The immune system comprises multiple cell lineages and heterogeneous subsets found in blood tissues throughout the body. While human responses differ between sites over age, underlying sources of variation remain unclear as most studies are limited to peripheral blood. Here, we took a systems approach comprehensively profile RNA surface protein expression 1.25 million cells isolated from blood, lymphoid organs, mucosal 24 organ donors aged 20-75 years. We applied multimodal classifier...
Ionic liquid (IL)-assisted pretreatment of lignocellulosic biomass has been extensively studied.
Abstract Tau phosphorylation shows direct clinical importance as the hyperphosphorylation and aggregation of tau exists in a range tauopathies. However, it is still challenging to study owing its multiple adjacent sites sequence. To address this challenge, here, designed T232K/K238Q mutant aerolysin nanopore introduced which synergistically incorporates enhanced electrostatic interaction at T232K site high repelling barrier K238Q site. The distinct current blockages produced by sensor...
Abstract Protein O−GlcNAcylation is a ubiquitous posttranslational modification of cytosolic and nuclear proteins involved in numerous fundamental regulation processes. Investigation by metabolic glycoengineering (MGE) has been carried out for two decades with peracetylated N ‐acetylglucosamine (GlcNAc) ‐acetylgalactosamine derivatives modified varying reporter groups. Recently, it shown that these can result non‐specific protein labeling termed S ‐glyco modification. Here, we report...
Hedgehog (Hh) transduces signal by regulating the subcellular localization and conformational state of GPCR-like protein Smoothened (Smo) but how Smo relays to cytoplasmic signaling components remains poorly understood. Here, we show that Hh-induced change recruits Costal2 (Cos2)/Fused (Fu) promotes Fu kinase domain dimerization. We find induced dimerization through activates inducing multi-site phosphorylation its activation loop (AL) phospho-mimetic mutations AL activate Hh pathway....
High mobility group box 1 (HMGB1) as a novel inflammatory molecule has been shown to be involved in variety of cell physiological and pathological behaviors including immune response, inflammation cancer. Evidence suggests that HMGB1 plays critical role the development progression multiple malignancies. However, underlying molecular mechanisms for HMGB1-mediated growth invasion gastric cancer have not yet elucidated. The present study investigated expression adenocarcinoma (GAC) by which it...
The concept of robustness in biology has gained much attention recently, but a mechanistic understanding how genetic networks regulate phenotypic variation remained elusive. One approach to understand the architecture variability been analyze dispensable gene deletions model organisms; however, most important genes cannot be deleted. Here, we have utilized two systems yeast whereby essential altered reduce expression. Using high-throughput microscopy and image analysis, characterized large...
Long non-coding RNAs can regulate tumorigenesis of various cancers. Dys-regulation lncRNA-AFAP1-AS1 has not been studied in colorectal carcinoma (CRC). This study was to examine the function involvement AFAP1-AS1 tumor growth and metastasis CRC. Relative expression CRC tissues cells lines determined using quantitative real-time PCR (qRT-PCR). Functional proliferation evaluated AFAP1-AS1-specific siRNA-treated cell xenograft. Expression epithelial-mesenchymal transition (EMT)-related gene...
Clones of excitatory neurons derived from a common progenitor have been proposed to serve as elementary information processing modules in the neocortex. To characterize cell types and circuit diagram clonally related neurons, we performed multi-cell patch clamp recordings Patch-seq on Nestin-positive progenitors labeled by tamoxifen induction at embryonic day 10.5. The resulting clones are two radial glia average, span cortical layers 2–6, composed random sampling transcriptomic types. We...
Pleiotropy-when a single mutation affects multiple traits-is controversial topic with far-reaching implications. Pleiotropy plays central role in debates about how complex traits evolve and whether biological systems are modular or organized such that every gene has the potential to affect many traits. is also critical initiatives evolutionary medicine seek trap infectious microbes tumors by selecting for mutations encourage growth some conditions at expense of others. Research these fields,...
Bread wheat (Triticum aestivum) is a staple food crop worldwide. The genetic dissection of important nutrient traits essential for the biofortification to meet nutritional needs world's growing population. Here, 45298 single-nucleotide polymorphisms (SNPs) from 55K chip arrays were used genotype panel 768 cultivars; total 154 quantitative trait loci (QTLs) detected eight under three environments by genome-wide association study (GWAS). Three QTLs (qMn-3B.1, qFe-3B.4, and qSe-3B.1/qFe-3B.6)...