A5012778267- Sinusitis and nasal conditions
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Oral microbiology and periodontitis research
- Bacteriophages and microbial interactions
- Nasal Surgery and Airway Studies
- Asthma and respiratory diseases
- Head and Neck Surgical Oncology
- Allergic Rhinitis and Sensitization
- Genomics and Phylogenetic Studies
- Microbial infections and disease research
- Infectious Diseases and Mycology
- Inhalation and Respiratory Drug Delivery
- Immune Response and Inflammation
- Otolaryngology and Infectious Diseases
- Antimicrobial Peptides and Activities
- Antimicrobial Resistance in Staphylococcus
- Hemostasis and retained surgical items
- Hearing, Cochlea, Tinnitus, Genetics
- Cystic Fibrosis Research Advances
- Gut microbiota and health
- Electrospun Nanofibers in Biomedical Applications
- Microbial Community Ecology and Physiology
- Barrier Structure and Function Studies
- Pneumonia and Respiratory Infections
The University of Adelaide
2016-2025
Basil Hetzel Institute
2016-2025
Central Adelaide Local Health Network
2020-2025
Queen Elizabeth Hospital
2015-2024
Women's and Children's Health Network
2022-2023
Health Net
2023
Roche (France)
2019
RELX Group (Netherlands)
2019
Hokkaido University
2018
South Australia Pathology
2018
Abstract Summary In recent years, there has been an increasing interest in bacteriophages, which led to growing numbers of bacteriophage genomic sequences becoming available. Consequently, is a need for rapid and consistent annotation tool dedicated bacteriophages. Existing tools either are not designed specifically bacteriophages or web- email-based require significant manual curation, makes their integration into bioinformatic pipelines challenging. Pharokka was created provide that...
Improvements in the accuracy and availability of long-read sequencing mean that complete bacterial genomes are now routinely reconstructed using hybrid (i.e. short- long-reads) assembly approaches. Complete allow a deeper understanding evolution genomic variation beyond single nucleotide variants. They also crucial for identifying plasmids, which often carry medically significant antimicrobial resistance genes. However, small plasmids missed or misassembled by algorithms. Here, we present...
It is now possible to assemble near-perfect bacterial genomes using Oxford Nanopore Technologies (ONT) long reads, but short-read polishing usually required for perfection. However, the effect of depth on performance not well understood. Here, we introduce Pypolca (with default and careful parameters) Polypolish v0.6.0 a new parameter). We then show that: (1) all polishers other than Pypolca-careful, Polypolish-default Polypolish-careful commonly false-positive errors at low read depth; (2)...
Normal vision in Drosophila requires NINAC, a class III myosin. Class myosins are hybrid motor-signaling molecules, with an N-terminal kinase domain, highly conserved head and neck domains, III-specific tail domain. In rhabdomeres, NINAC interacts actin filaments PDZ scaffolding protein to organize the phototransduction machinery into signaling complex. Recessive null mutations delay termination of photoreceptor response lead progressive retinal degeneration. Here, we show that normal...
Abstract Background Group 2 innate lymphoid cells ( ILC 2s) were shown to be involved in the initiation and coordination of Th2‐type immune responses allergic disease animal models. Recently, 2s enrichment was noted chronic rhinosinusitis CRS ) patients; however, role coordinating Th2 response remains elucidated. Here, we characterize compartment by investigating correlations between 2s, cytokines expression patients. Methods We used flow cytometric analysis sinonasal mucosal tissues 29...
<h3>Importance</h3> <i>Staphylococcus aureus</i>infections are associated with recalcitrant chronic rhinosinusitis (CRS). The emerging threat of multidrug-resistant<i>S has revived interest in bacteriophage (phage) therapy. <h3>Objective</h3> To investigate the safety, tolerability, and preliminary efficacy ascending multiple intranasal doses investigational phage cocktail AB-SA01 patients CRS due to<i>S aureus</i>. <h3>Design, Setting, Participants</h3> This phase 1, first-in-humans,...
Introduction: Pseudomonas aeruginosa infections are prevalent amongst chronic rhinosinusitis (CRS) sufferers. Many P. strains form biofilms, leading to treatment failure. Lytic bacteriophages (phages) viruses that infect, replicate within, and lyse bacteria, causing bacterial death. Aim: To assess the activity of a phage cocktail in eradicating biofilms ex vivo isolates from CRS patients. Methods: patients with without cystic fibrosis (CF) across three continents were multi-locus sequence...
With recent advances in sequencing technologies, it is now possible to obtain near-perfect complete bacterial chromosome assemblies cheaply and efficiently by combining a long-read-first assembly approach with short-read polishing. However, existing methods for assembling plasmids from often misassemble or even miss entirely accordingly require manual curation. Plassembler was developed provide tool that automatically assembles outputs using hybrid approach. It achieves increased accuracy...
Abstract It is now possible to assemble near-perfect bacterial genomes using Oxford Nanopore Technologies (ONT) long reads, but short-read polishing still required for perfection. However, the effect of depth on performance not well understood. Here, we introduce Pypolca (with default and careful parameters) Polypolish v0.6.0 a new parameter). We then show that: (1) all polishers other than Pypolca-careful, Polypolish-default Polypolish-careful commonly false-positive errors at low depth;...
Chronic rhinosinusitis (CRS) is a prevalent inflammatory condition that substantially affects patients' quality of life. Functional endoscopic sinus surgery (FESS) commonly performed in cases where medical therapy fails. Effective post-operative drug delivery crucial for improving outcomes. This study uses computational fluid dynamics (CFD) to compare nebulisation and nasal irrigation (bottle wash) sinonasal models evaluate how anatomical alterations affect deposition across various...
Animal models that can mimic progressive granulomatous pulmonary disease (PD) due to non-tuberculous mycobacteria (NTM) have not been established in rats date. These could assist with the study of pathophysiology NTM-PD as well preclinical development new therapies. In present study, an immunocompetent rat model Mycobacterium abscessus (MABs)- PD was developed using MABs originating from a patient cystic fibrosis. embedded agarose beads and delivered intratracheally lungs Sprague Dawley two...
Background Staphylococcus aureus and Pseudomonas aeruginosa are major pathogens in chronic rhinosinusitis (CRS) their biofilms have been associated with poorer postsurgical outcomes. This study investigated the distribution anti-biofilm effect of cationic (+) anionic (-) phospholipid liposomes different sizes (unilamellar multilamellar vesicle, ULV MLV respectively) on S. P. biofilms. Method Specific biofilm models for ATCC 25923 15692 were established. Liposomal was determined by observing...
Background Our understanding of fungi in chronic rhinosinusitis (CRS) has been limited by previously employed detection techniques. This study examines the fungal component microbiome CRS patients and controls using a highly sensitive culture‐independent molecular technique. The aims this include characterization richness, prevalence, abundance, temporal changes, their relationship with patient outcomes. Methods Swabs were collected from sinuses 23 11 controls. Collection occurred...
Cytokine mediated changes in paracellular permeability contribute to a multitude of pathological conditions including chronic rhinosinusitis (CRS). The purpose this study was investigate the effect interferons and Th1, Th2, Th17 cytokines on respiratory epithelium barrier function. Cytokines were applied basolateral side air-liquid interface (ALI) cultures primary human nasal epithelial cells (HNECs) from CRS with polyp patients. Transepithelial electrical resistance (TEER) FITC-conjugated...
Background The bacterial microbiome in chronic rhinosinusitis (CRS) remains poorly understood. Microorganisms are believed to be important contributors the inflammatory response seen these patients. Objective To examine CRS by using a pyrosequencing technique and determine diversity, richness, prevalence, abundance of species Furthermore, postoperative changes that occur correlations with patient outcomes assessed. Methods Swabs were collected from 23 patients 11 controls during surgery....
Abstract The sinonasal microbiome remains poorly defined, with our current knowledge based on a few cohort studies whose findings are inconsistent. Furthermore, the variability of sinus across geographical divides unexplored. We characterize and its variations in both health disease using 16S rRNA gene sequencing 410 individuals from world. Although microbial ecology is highly variable between individuals, we identify core comprised Corynebacterium , Staphylococcus Streptococcus Haemophilus...
Many infectious diseases are associated with multidrug-resistant (MDR) bacteria residing in biofilms that require high antibiotic concentrations. While oral drug delivery is frequently ineffective, topical treatments have the potential to deliver higher concentrations infection site while reducing systemic side effects. This study determined antibiofilm activity of a surgical wound gel loaded iron chelator deferiprone (Def) and heme analogue gallium-protoporphyrin (GaPP), alone combination...
The adenoid pad has been considered a reservoir for bacteria in the pathogenesis of otitis media with effusion. This study aimed to characterize middle ear microbiota children effusion and establish whether correlation exists between microbiota.Prospective, controlled study.Middle aspirates swabs were collected from 23 undergoing ventilation tube insertion. Adenoid patients without disease controls. Samples analyzed using 16S rRNA sequencing on Illumina MiSeq platform.Thirty-five samples...
Corynebacterium accolens is the predominant species of healthy human nasal microbiota, and its relative abundance decreased in context chronic rhinosinusitis (CRS). This study aimed to evaluate antimicrobial potential C. isolated from a cavity against planktonic biofilm growth Staphylococcus aureus (S. aureus) methicillin-resistant S. (MRSA) clinical isolates (CIs) CRS patients. Nasal swabs twenty non-CRS control subjects were screened for presence using microbiological molecular techniques....