A5100369298- Bone Metabolism and Diseases
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Bone health and treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer-related Molecular Pathways
- Histone Deacetylase Inhibitors Research
- Reproductive biology and impacts on aquatic species
- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Metal-Organic Frameworks: Synthesis and Applications
- Sulfur Compounds in Biology
- Alkaline Phosphatase Research Studies
- Aerogels and thermal insulation
- Nanocluster Synthesis and Applications
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hematopoietic Stem Cell Transplantation
- Genetics and Physical Performance
- Sphingolipid Metabolism and Signaling
- Hepatocellular Carcinoma Treatment and Prognosis
- Circular RNAs in diseases
Tangshan College
2024
Beijing University of Chinese Medicine
2024
Nanjing University of Chinese Medicine
2017-2023
Tianjin University of Science and Technology
2023
Jiujiang University
2012-2022
Henan University of Engineering
2022
Xiangya Hospital Central South University
2020
Central South University
2020
Ghent University
2019
Guangdong Pharmaceutical University
2018
Background With increasing knowledge about the gut – bone axis, more studies for treatments based on regulation of postmenopausal osteoporosis by microbes are being conducted. Based our previous work, this study was conducted to further investigate therapeutic effects Lactobacillus rhamnosus GG (LGG) ovariectomized (OVX) model rats and immunological microecological mechanisms involved.Results We found a protective effect LGG treatment in OVX through changes microarchitecture, biomechanics,...
Molecular changes underlying stem cell differentiation are of fundamental interest. scRNA-seq on murine hematopoietic cells (HSC) and their progeny MPP1 separated the into 3 main clusters with distinct features: active, quiescent, an un-characterized cluster. Induction anemia resulted in mobilization quiescent to active cluster early later stage cycle, marked increase expression certain transcription factors (TFs) while maintaining interferon response genes. Cells surface markers long term...
Abstract Background The process of bone repair is heavily dependent on the ability human marrow mesenchymal stem cells (hMSCs) to undergo osteogenic differentiation. MicroRNAs have been shown regulate this process. This study aimed investigate role miR-765 in differentiation hMSCs. Methods We transfected hMSCs with lentiviral constructs knock down or overexpress miRNA, allowing us assess its osteogenesis via assessing expression relevant markers alkaline phosphatase (ALP), runt-related...
Reduction-responsive core-crosslinked micelles were developed based on a glycol chitosan–lipoic acid (GC–LA) conjugate and used for triggered release of doxorubicin (DOX). The substitution degree GC–LA was 8.3 lipoic groups per 100 sugar units chitosan. could form nanoscaled in aqueous solution, wherein the critical micelle concentration (CMC) 0.081 mg mL−1 determined. Furthermore can be crosslinked by catalytic amount dithiothreitol. mean diameter DOX-loaded (DOX-GC–LA/cc) increased from...
Abstract MicroRNAs (miRNAs), the potential regulator of adipogenesis, markedly characterized by lipid droplet (LD) formation, play an important role in progenitor‐cell differentiation into adipocytes. In recent years, it has excited interests regulation miRNAs adipogenesis. However, no study is available, to our knowledge, regarding expression on LD formation. Our provides first insight profiling miRNA targeting messenger RNAs (mRNAs) involving with formation during adipogenesis from human...
Abstract Adipogenesis, a physiological process initiated with the committed preadipocytes expressing adipocyte‐specific genes and terminated in mature, differentiated functional adipocytes, mainly involved energy homeostasis. Abnormal distribution‐changes dysfunctions adipogenesis may lead to complex physiopathological disorders. However, it remains unclear for key players working whole differentiating of adipogenesis. Here, investigated transcriptional profiling from human mesenchymal stem...
Polysaccharide from Fuzi (FPS) is a water‑soluble polysaccharide isolated the traditional Chinese herbal medicine Fuzi. It has been demonstrated to protect hepatocytes against ischemia‑reperfusion injury through its potent antioxidant effects, and attenuate starvation‑induced cytotoxicity in H9c2 cells by increasing autophagic activity. In present study, Alizarin Red S staining was used detect mineral deposition reverse transcription‑quantitative polymerase chain reaction core binding factor...
Adipogenesis comprises multiple processes by which mesenchymal stem cells differentiate into adipocytes. To increase our knowledge of the mechanism underlying adipogenic differentiation human bone marrow (hMSCs), we performed full-genome gene expression microarray and ontology analyses induced hMSCs.Adipogenic hMSCs was an medium, total RNA extracted from undifferentiated (day 0) differentiated adipocytes 14). Then hybridization samples performed. The GeneChip Operating Software used to...
Abstract Transfer RNA‐derived small RNAs (tsRNAs), a novel type of non‐coding RNA derivative, are able to regulate wide range biological processes. What role these tsRNAs play in the regulation human bone marrow mesenchymal stem cell (hMSCs) adipogenic differentiation remains uncertain. We induced cells and then performed transcriptomic sequencing, leading us identify tsRNA‐06018 as target interest based upon resultant tsRNA expression profiles. When was knocked down, this led inhibition...
Abstract Metabolism homeostasis plays an important role in progenitor‐cell differentiation to adipocytes, but less is known about the whole transcriptional profiling of cellular metabolism during adipogenesis. We got first insight into adipogenesis from human mesenchymal stem cells (hMSCs) by RNA‐Seq technique. There were 1,998, 2,629, 3,112, and 3,054 differentially expressed genes (DEGs) at Days 7, 14, 21, 28, respectively, The most enriched phosphatidylinositol 3′ kinase–serine/threonine...
Ubiquitin-specific processing enzyme 22 (USP22) plays a direct role in regulating cell cycle, and its overexpression has been reported to be involved tumor progression. However, little is known about the regulation of USP22 transcription. In this study, we cloned characterized human promoter. Using 5' RACE (rapid amplification cDNA ends) analysis, transcriptional initiation site was identified. Promoter deletion analysis showed that sequence between -210 -7 contains basal promoter for...
Abstract Adipogenesis, the developmental process of progenitor‐cell differentiating into adipocytes, leads to fat metabolic disorders. Alternative splicing (AS), a ubiquitous regulatory mechanism gene expression, allows generation more than one unique messenger RNA (mRNA) species from single gene. Till now, alternative events during adipogenesis human mesenchymal stem cells (hMSCs) are not yet fully elucidated. We performed RNA‐Seq coupled with bioinformatics analysis identify differentially...
The ubiquitin‑specific protease 22 (USP22) gene is overexpressed in the majority of types cancer cells, and has been implicated tumorigenesis. However, mechanisms that regulate its expression remain unclear. results present study demonstrated USP22 negatively regulated by trichostatin A (TSA), a classical histone deacetylase inhibitor. Furthermore, TSA was revealed to interfere with binding RNA polymerase II promoter, directly suppressing transcription. In addition, overexpression observed...
Adipogenesis and osteoblastogenesis (adipo-osteoblastogenesis) are closely related processes involving with the phosphorylation of numerous cytoplasmic proteins key transcription factors.
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