A5102970669- Mitochondrial Function and Pathology
- Cell death mechanisms and regulation
- Heat shock proteins research
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Lipid Membrane Structure and Behavior
- Autophagy in Disease and Therapy
- Bacillus and Francisella bacterial research
- Cancer therapeutics and mechanisms
- Calpain Protease Function and Regulation
- Bioactive Natural Diterpenoids Research
- Galectins and Cancer Biology
- Temporomandibular Joint Disorders
- Cellular Mechanics and Interactions
- Cardiomyopathy and Myosin Studies
- Osteoarthritis Treatment and Mechanisms
- Advanced Drug Delivery Systems
- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
- Bone and Dental Protein Studies
- Medical Imaging Techniques and Applications
- interferon and immune responses
- Epigenetics and DNA Methylation
- Endoplasmic Reticulum Stress and Disease
- Bioactive Compounds and Antitumor Agents
Université d'Angers
2010-2025
Inserm
2004-2024
Centre National de la Recherche Scientifique
1999-2010
Université Claude Bernard Lyon 1
1999-2010
Nantes Université
2008
Centre de Génétique Moléculaire
2001-2002
We previously showed that Hsp27 protects against apoptosis through its interaction with cytosolic cytochrome c. have revisited this protective activity in murine cell lines expressing different levels of Hsp27. report also interferes, a manner dependent on level expression, the release c from mitochondria. Moreover, decreased endogenous Hsp27, which sensitized HeLa cells to apoptosis, reduced delay required for and procaspase 3 activation. The molecular mechanism regulating function is...
Abstract A functional imbalance between proapoptotic Bax and antiapoptotic Bcl-2 is likely to participate in the resistance of cancer cells therapy. We show here that ethyl 2-amino-6-bromo-4-(1-cyano-2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate (HA14-1), a small organic compound recently proposed function as an inhibitor Bcl-2, increases sensitivity human glioblastoma radiotherapy chemotherapy. This sensitizing effect lost if expression, but not Bcl-xL knocked down or only express mutant...
Abstract Lactic acidosis, the extracellular accumulation of lactate and protons, is a consequence increased glycolysis triggered by insufficient oxygen supply to tissues. Macrophages are able differentiate from monocytes under such acidotic conditions, remain active in order resolve underlying injury. Here we show that, lactic human differentiating into macrophages characterized depolarized mitochondria, transient reduction mitochondrial mass due mitophagy, significant decrease nutrient...
Impaired spermatogenesis and male infertility are common manifestations associated with mitochondrial diseases, yet the underlying mechanisms linking these conditions remain elusive. In this study, we demonstrate that mice deficient for intra-membrane rhomboid protease PARL, a recently reported model of encephalopathy Leigh syndrome, develop early testicular atrophy caused by complete arrest during meiotic prophase I, followed degeneration death arrested spermatocytes. This process is...
Mitochondrial dynamics and distribution are critical for supplying ATP in response to energy demand. CLUH is a protein involved mitochondrial whose dysfunction leads clustering, the metabolic consequences of which remain unknown. To gain insight into role on production cellular metabolism, we have generated CLUH-knockout cells using CRISPR/Cas9. clustering was associated with smaller cell size decreased abundance respiratory complexes, resulting oxidative phosphorylation (OXPHOS) defects....
Abstract: Development of effective antibacterial agents for the treatment infections caused by Gram-positive bacteria resistant to existing antibiotics, such as methicillin-resistant Staphylococcus aureus (MRSA), is an area intensive research. In this work, efficacy two antimicrobial peptides derived from plectasin, AP114 and AP138, used alone in combination with monolaurin-lipid nanocapsules (ML-LNCs) was evaluated. Several interesting findings emerged present study. First, ML-LNCs both...
Abstract Background myotilinopathy is a very rare inherited muscle disease that belongs to the group of myofibrillar myopathies. These diseases share common alteration sarcomere organization at level Z disk resulting in pathological protein aggregation, autophagic abnormalities, and ultimately degeneration. Most reported cases are due dominant missense mutations MYOT gene, two which largely recurrent. Methods We describe clinical, radiological, pathological, molecular analysis including...
Standard models used for evaluating the absorption of nanoparticles like Caco-2 ignore presence vascular endothelium, which is a part intestinal multi-layered barrier structure. Therefore, coculture between epithelium and HMEC-1 (Human Microvascular Endothelial Cell type 1) on Transwell® insert has been developed. The model validated (a) membrane morphology by transmission electron microscope (TEM); (b) ZO-1 β-catenin expression immunoassay; (c) integrity trans-epithelial electrical...
Hereditary optic neuropathies, including dominant atrophy and Leber's hereditary neuropathy, are genetic disorders characterized by retinal ganglion cell degeneration leading to vision loss, mainly associated with mitochondrial dysfunction. In this study, we analysed distribution ultrastructure in the retina longitudinal nerve sections of pre-symptomatic neuropathies mouse models Opa1 Nd6 deficiency identify early changes. Our results show significant fragmentation increased mitophagy
Abstract Background MCC/eisosomes are membrane microdomains that have been proposed to participate in the plasma function particular by regulating homeostasis of lipids, promoting recruitment specific proteins and acting as provider reservoirs. Results Here we showed several potential MCC/eisosomal protein encoding genes necrotrophic fungus A. brassicicola were overexpressed when germinated spores exposed antimicrobial defence compounds, osmotic hydric stresses, which major constraints...
Introduction: Artificial Extracellular Matrices (ECMs) are promising tools for the study of cell behaviors. Methods: Here, we report a protocol use reconfigurable biocompatible azopolymer thin film through photoinduced structuration its surface to nerve growth, differentiation and guidance. Results & Discussion: We show that this combined with spontaneous self-photoinduced polymer is suitable time-lapse fluorescence video microscopy can be easily adapted electron techniques (SEM)...