A5005966251- Cannabis and Cannabinoid Research
- Neuroscience and Neuropharmacology Research
- Pancreatic function and diabetes
- Peroxisome Proliferator-Activated Receptors
- Sleep and Wakefulness Research
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Alcohol Consumption and Health Effects
- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Alcoholism and Thiamine Deficiency
- Forensic Toxicology and Drug Analysis
- Diabetes Treatment and Management
- Neurotransmitter Receptor Influence on Behavior
- Tryptophan and brain disorders
- Liver Disease Diagnosis and Treatment
- Diet, Metabolism, and Disease
- Epigenetics and DNA Methylation
- Heme Oxygenase-1 and Carbon Monoxide
- Inflammatory mediators and NSAID effects
- Lysosomal Storage Disorders Research
- Moringa oleifera research and applications
- Enhanced Recovery After Surgery
- Metabolism and Genetic Disorders
- Immune cells in cancer
University of L'Aquila
2022-2025
European Brain Research Institute
2017-2025
Fondazione Santa Lucia
2019-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2019
Università Campus Bio-Medico
2017-2018
The endocannabinoid N ‐arachidonoylethanolamine (AEA) is a pro‐homeostatic bioactive lipid known for its anti‐inflammatory, anti‐oxidative, immunomodulatory, and neuroprotective properties, which may contrast/mitigate Alzheimer's disease (AD) pathology. This study explores the therapeutic potential of targeting fatty acid amide hydrolase (FAAH), major enzyme degrading AEA, in mouse models amyloidosis (APP/PS1 Tg2576). Enhancing AEA signaling by genetic deletion FAAH delayed cognitive...
5-lipoxygenase (5-LOX) is a non-heme iron-containing dioxygenase expressed in immune cells that catalyzes the two initial steps biosynthesis of leukotrienes. It well known 5-LOX activation innate immunity related to different iron-associated pro-inflammatory disorders, including cancer, neurodegenerative diseases and atherosclerosis. However, molecular cellular mechanism(s) underlying interplay between iron are largely unexplored. In this study, we investigated whether (in form Fe3+ hemin)...
Growing evidence shows that the immune system is critically involved in Alzheimer’s disease (AD) pathogenesis and progression. The modulation targeting of peripheral mechanisms are thus promising therapeutic or preventive strategies for AD. Given critical involvement endocannabinoid (eCB) modulating functions, we investigated potential role main elements such a system, namely type-1 type-2 cannabinoid receptors (CB1 CB2), fatty acid amide hydrolase (FAAH), distinct cell populations blood AD...
The present study encompasses the development of a fast and reliable analytical method to quantify main endocannabinoids some their conjugated congeners, particularly N-arachidonoyl amino acids, in brain tissue. Samples were homogenized micro solid phase extraction (μSPE) procedure was developed for homogenate clean-up. Miniaturized SPE selected as it allowed work with reduced sample amounts, while maintaining high sensitivity; this last feature mandatory due low concentration biological...
Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cognitive and non-cognitive decline associated with neuropathological hallmarks, including neuroinflammation. Palmitoylethanolamide (PEA), an endogenous lipid anti-inflammatory neuroprotective properties, has emerged as promising therapeutic agent in managing AD. This study investigated the effects of chronic (6-months) PEA administration via subcutaneous pellet Tg2576 mice, validated model The impact on...
Abstract Resolution of inflammation is the cellular and molecular process that protects from widespread uncontrolled restores tissue function in aftermath acute immune events. This orchestrated by specialized pro‐resolving mediators (SPM), a class bioactive lipids able to reduce activation promote removal debris apoptotic cells macrophages. Although SPMs are lipid has been best studied for its role facilitating resolution self‐limited inflammation, number other signals, including...
In this study, we investigated the role of CB1 palmitoylation in modulating functional interaction with G proteins both absence and presence agonist binding. Our data show that nonpalmitoylated receptor significantly reduced its association Gαi2 . The stimulation induced a partial dissociation from wild-type receptor, while on C415A mutant binding was not able to induce significant receptor. lack palmitoyl chain seems hamper ability functionally interact indicate is responsible for...
The diversity of physiological roles the endocannabinoid system has turned it into an attractive yet elusive therapeutic target. However, chemical probes with various functionalities could pave way for a better understanding at cellular level. Notably, inverse agonists CB2R – key receptor - lagged behind despite evidence regarding potential its antagonism. Herein, we report matched fluorescent probe pair based on common chemotype to address and visualize both active inactive states CB2R,...
Neuroinflammation is a hallmark of several neurodegenerative disorders that has been extensively studied in recent years. Microglia, the primary immune cells central nervous system (CNS), are key players this physiological process, demonstrating remarkable adaptability responding to various stimuli eye and brain. Within complex network neuroinflammatory signals, fatty acid N-ethanolamines, particular N-arachidonylethanolamine (anandamide, AEA), emerged as crucial regulators microglial...
Abstract The endocannabinoid N -arachidonoylethanolamine (AEA) is a pro-homeostatic bioactive lipid known for its anti-inflammatory, anti-oxidative, immunomodulatory, and neuroprotective properties, which may contrast/mitigate Alzheimer’s disease (AD) pathology. This study explores the therapeutic potential of targeting fatty acid amide hydrolase (FAAH), major enzyme degrading AEA, in mouse models amyloidosis APP/PS1 Tg2576. Enhancing AEA signaling by genetic deletion FAAH delayed cognitive...
Dysfunctional phenotype of microglia, the primary brain immune cells, may aggravate Alzheimer's disease (AD) pathogenesis by releasing proinflammatory factors, such as nitric oxide (NO). The endocannabinoids N-arachidonoylethanolamine (AEA) and 2-arachidonoylglycerol (2-AG) are bioactive lipids increasingly recognised for their essential roles in regulating microglial activity both under normal AD-driven pathological conditions. To investigate possible impact chronic exposure to β-amyloid...
Abstract We investigated the cellular and molecular mechanisms by which bindarit, a small indazolic derivative with prominent anti-inflammatory effects, exerts its immunoregulatory activity in lipopolysaccharide (LPS) stimulated human monocytic cells. found that bindarit differentially regulates release of interleukin-8 (IL-8) monocyte chemoattractant protein-1 (MCP-1), enhancing IL-8 reducing MCP-1. These effects specifically required functional interaction between fatty acid binding...
N-palmitoylethanolamine (PEA) plays a key role in preventing Aβ-mediated neuroinflammation and neurotoxicity murine models. It has been demonstrated that PEA provides anti-neuroinflammatory, pain-relieving neuroprotective actions even humans. In this project, we aim to evaluate these anti-neuroinflammatory effects via the cognitive evaluation biochemical analyses of 12-month oral administration subjects with mild impairment (MCI). Subjects MCI will be randomized placebo or groups, followed...
The management of gastrointestinal disease in animals represents a significant challenge veterinary and zootechnic practice. Traditionally, acute symptoms have been treated with antibiotics high doses zinc oxide (ZnO). However, concerns raised regarding the potential for microbial resistance ecological detriment due to excessive application this compound. These highlight urgency minimizing use ZnO exploring sustainable nutritional solutions. Hydrolysable tannins (HTs), which are known their...
Abstract Extra‐cerebral manifestations of Alzheimer's disease (AD) develop in the retina, which is, therefore, considered a “window to brain”. Recent studies demonstrated dysregulation endocannabinoid (eCB) system (ECS) AD brain. Here, we explored possible alterations ECS and onset gliosis retina AD‐like mice. Tg2576 (TG) mice overexpressing amyloid precursor protein (APP) were used at age 12 months, when hippocampal β‐amyloid plaques had not been developed yet. Analysis retinal showed...
In the symptomatic treatment of gastrointestinal disorders, high dosages zinc oxide (ZnO) were conventionally employed. However, concerns regarding potential for microbial resistance and ecological detriment have arisen due to excessive application this compound. These highlight urgency minimize use ZnO explore sustainable nutritional solutions. Hydrolysable tannins (HTs), known their role in traditional medicine acute issues, emerge as a promising alternative. This study examined combined...
The diversity of physiological roles the endocannabinoid system has turned it into an attractive yet elusive therapeutic target. However, chemical probes with various functionalities could pave way for a better understanding at cellular level. Notably, inverse agonists CB