A5101409375- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Circular RNAs in diseases
- Lung Cancer Treatments and Mutations
- Lung Cancer Diagnosis and Treatment
- Mesenchymal stem cell research
- Protein Hydrolysis and Bioactive Peptides
- Insect Utilization and Effects
- Lung Cancer Research Studies
- Aquaculture Nutrition and Growth
- Radiomics and Machine Learning in Medical Imaging
- Epigenetics and DNA Methylation
- Gastric Cancer Management and Outcomes
- Cancer-related gene regulation
- Cancer Immunotherapy and Biomarkers
- Cell death mechanisms and regulation
- Tissue Engineering and Regenerative Medicine
- Drug Transport and Resistance Mechanisms
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA Research and Splicing
- Bioactive Compounds and Antitumor Agents
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Acupuncture Treatment Research Studies
- Heat shock proteins research
Zhoushan Hospital
2015-2024
Wenzhou Medical University
2016-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Second Affiliated Hospital of Zhejiang University
2022
Huashan Hospital
2019
Fudan University
2019
Affiliated Hospital of Nantong University
2018
Nantong University
2018
Zhejiang Cancer Hospital
2013-2014
Zhengzhou University
2014
Lung cancer is the leading cause of cancer-related deaths worldwide and non-small cell lung (NSCLC) accounts for over 80% cases. The RNA binding protein, QKI, belongs to STAR family plays tumor-suppressive functions in NSCLC. QKI-5 a major isoform QKIs predominantly expressed However, underlying mechanisms NSCLC progression remain unclear. We found that regulated microRNA (miRNA), miR-196b-5p, its expression was significantly up-regulated tissues. Up-regulated miR-196b-5p promotes migration,...
In the study, papain was chosen from five proteases to hydrolyze proteins of monkfish swim bladders for effectively utilizing (Lophius litulon) processing byproducts, and hydrolysis conditions were optimized as temperature 65 °C, pH 7.5, enzyme dose 2.5% time 5 h using single-factor orthogonal experiments. Eighteen peptides purified bladder hydrolysate by ultrafiltration gel permeation chromatography methods identified YDYD, QDYD, AGPAS, GPGPHGPSGP, GPK, HRE, GRW, ARW, GPTE, DDGGK, IGPAS,...
Background Altered expression of miRNA contributes to human carcinogenesis. This study was designed detect aberrant expressions as a potential biomarker for early detection and prognosis prediction non-small cell lung cancer (NSCLC). Methods array used profile differentially expressed miRNAs Taqman-based quantitative RT-PCR assays were analyze levels miR-29c, miR-93, miR-429 in NSCLC tissue samples, corresponding normal serum samples from 70 patients well 48 healthy controls. Results Levels...
Blood-circulating miRNAs could be useful as a biomarker to detect lung cancer early. We investigated the serum levels of four different in patients with non-small cell (NSCLC) and assessed their diagnostic value for NSCLC. Serum samples from 112 NSCLC 104 controls (20 current smokers without cancer, 23 pneumonia patients, 21 gastric 40 healthy controls) were subjected Taqman probe-based quantitative reverse transcription–polymerase chain reaction (RT-PCR). The data showed that miR-182,...
Abstract Background Lung cancer is the leading cause of cancer-related deaths worldwide. Early detection considered critical for lung treatment. MicroRNAs (miRNAs) have shown promise as diagnostic and prognostic indicators. This study was to identify specific miRNAs with value patients cancer, explore correlation between expression profiles patient survival. Methods Gene members miR-183 family (miR-96, miR-182, miR-183) were examined in 70 paired samples from (primary non-cancerous tissues...
Using DNA microarrays, we generated both mRNA and miRNA expression data from 6 non-small cell lung cancer (NSCLC) tissues their matching normal control adjacent to identify potential markers for diagnostics. We demonstrated that hsa-miR-96 is significantly consistently up-regulated in all NSCLCs. validated this result an independent set of 35 paired tumors tissues, as well sera are collected before surgical resection or chemotherapy, the results suggested may play important role NSCLC...
Background Methylated DNA in fluids may be a suitable biomarker for cancer patients. XAF1 has been shown to frequently down-regulated human gastric (GC). Here, we investigated if methylation GC could useful biomarker. Methods Real-time RT-PCR was used detect mRNA expression; immunohistochemistry and western blot were examine protein expression tissues (n = 202) their corresponding para-cancerous histological normal (PCHNTs). specific-PCR investigate promoter the same panel of tissues, PCHNTs...
Dihydroartemisinin (DHA) exerts an anti-tumor effect in multiple cancers, however, the molecular mechanism of DHA and whether facilitates efficacy cisplatin non-small cell lung cancer (NSCLC) are unclear. Here, we found that potentiated effects NSCLC cells by stimulating reactive oxygen species (ROS)-mediated endoplasmic reticulum (ER) stress, C-Jun-amino-terminal kinase (JNK) p38 MAPK signaling pathways both vitro vivo. Of note, demonstrated for first time inhibits prostaglandin G/H...
Abstract Our recent study demonstrated that the QKI-5 regulated miRNA, miR-196b-5p , and it functions as an onco-microRNA in non-small cell lung cancer (NSCLC) by directly targeting GATA6 TSPAN12. However, role of NSCLC progression metastasis still remains unclear. We found promotes proliferation colony formation tumor suppressor, FAS. The expression FAS was significantly downregulated tissue samples negatively correlated with expression. Knocking down activates NFkB signaling subsequent IL6...
Fluid methylated DNA may be a suitable biomarker for cancer patients.To investigate whether circulating tissue inhibitor of metalloproteinase 3 (TIMP-3) in body fluids is useful prognostic gastric (GC).TIMP-3 methylation was detected by real-time methylation-specific polymerase chain reaction tumor tissues, paired preoperative peritoneal washes (PPWs), and serum samples from 92 GC patients.The frequency TIMP-3 significantly elevated tissues (63.04%; 58 92) compared with that adjacent normal...
Mounting evidence has shown that a novel subset of mesenchymal stem cells (MSCs) derived from human gingiva referred to as gingival (GMSCs) displays greater immunotherapeutic potential and regenerative repair expression than MSCs obtained other tissues. However, the safety use transplanted GMSCs in humans remains unclear. In this study, we evaluated into mouse, rat, rabbit, beagle dog, monkey well two animal models autoimmune diseases. short- long-term toxicity tests, infused had no...
Abstract Triple‐negative breast cancer (TNBC) is a subtype of with poor clinical outcome and currently no effective targeted therapies are available. Alantolactone (ATL), sesquiterpene lactone, has been shown to have potential anti‐tumour activity against various cells. However, the underlying mechanism therapeutic effect ATL in TNBC largely unknown. In present study, we found that suppresses cell viability by reactive oxygen species (ROS) accumulation subsequent ROS‐dependent endoplasmic...
For efficiently utilizing the processing byproducts of miiuy croaker to prepare novel angiotensin I-converting enzyme (ACE) inhibitory (ACEi) peptides, in vitro gastrointestinal (GI) digestion method was screened and employed swim bladder hydrolysate with highest ACEi activity. Subsequently, seven peptides were isolated from identified as DEGPE, EVGIQ, SHGEY, GPWGPA, GPFGTD, SPYGF, VIGPF molecular weights 545.49, 544.58, 591.55, 583.63, 592.59, 569.60, 531.63 Da, respectively. SHGEY SPYGF...
Background Exceeding 50% tuna catches are regarded as byproducts in the production of cans. Given high amount and their environmental effects induced by disposal elimination, valorization nutritional ingredients from these by-products receives increasing attention. Objective This study was to identify angiotensin-I-converting enzyme (ACE) inhibitory (ACEi) peptides roe hydrolysate Skipjack ( Katsuwonus pelamis ) evaluate protection functions on H 2 O -induced human umbilical vein endothelial...
Abstract Metastasis and multidrug resistance (MDR) are the main reasons for poor prognosis of non‐small cell lung cancer (NSCLC) patients. The use biomarkers may contribute to a more accurate prediction tumor metastasis, better response chemotherapy, patient survival. Gelsolin‐like actin‐capping protein (CapG) gelsolin have been identified as playing important roles in invasion metastasis. Permeability glycoprotein (P‐gp), glutathione S‐transferase pi (GSTP1), topoisomerase‐II (Topo‐II)...
Non‑small cell lung cancer (NSCLC) is one of the major causes cancer‑related death worldwide. Cisplatin a front‑line chemotherapeutic agent in NSCLC. Nevertheless, subsequent harsh side effects and drug resistance limit its further clinical application. Polydatin (PD) induces apoptosis various cells by generating reactive oxygen species (ROS). However, underlying molecular mechanisms PD on cisplatin‑mediated antitumor activity NSCLC remains unknown. MTT, colony formation, wound healing...