A5101724923- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Protein Tyrosine Phosphatases
- RNA and protein synthesis mechanisms
- Bioactive Compounds and Antitumor Agents
- CRISPR and Genetic Engineering
- Protein Degradation and Inhibitors
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Natural product bioactivities and synthesis
- Proteins in Food Systems
- Protein Hydrolysis and Bioactive Peptides
- Nutrition, Genetics, and Disease
- Click Chemistry and Applications
- Ferroptosis and cancer prognosis
- Endoplasmic Reticulum Stress and Disease
- Protein purification and stability
- Galectins and Cancer Biology
- Collagen: Extraction and Characterization
- Phytochemistry and Bioactive Compounds
- Transgenic Plants and Applications
- Aquaculture Nutrition and Growth
- Polysaccharides and Plant Cell Walls
- Postharvest Quality and Shelf Life Management
- Microbial Inactivation Methods
Guiyang Medical University
2021-2025
National University of Malaysia
2023-2025
Scripps (United States)
2022-2023
Scripps Institution of Oceanography
2022-2023
Scripps Research Institute
2021-2023
Tianjin Medical University
2020-2023
Peking University
2018-2020
The Wallace H. Coulter Department of Biomedical Engineering
2019-2020
Georgia Institute of Technology
2019-2020
Broad Institute
2018-2019
Whether genetic susceptibility to type 2 diabetes is modified by a healthy lifestyle among Chinese remains unknown.
CRISPR-associated proteins (Cas) are enabling powerful new approaches to control mammalian cell functions, yet the lack of spatially defined, noninvasive modalities limits their use as biological tools. Here, we integrate thermal gene switches with dCas9 complexes confer remote activation and suppression short pulses heat. Using a switch constructed from heat shock protein A6 (HSPA6) locus, show that single pulse 3-5 °C above basal temperature is sufficient trigger expression complexes. We...
Curcumin-loaded alkali-induced fish scale gelatin (AFSG) was fabricated to evaluate its efficacy as a potential carrier for hydrophobic nutrients. In this study, the effect of alkali hydrolysis period on AFSG hydrolysate structure and corresponding curcumin loading efficiency have been elucidated. Results showed that degradation yields different polymers with molecular weights (Mw) from 19319 3881 Da. Moderate exposes amino acids, enhancing interactions increasing proportion these acids....
Two new dibenz[b,f]oxepins, empetroxepins A and B (1 2), seven known compounds (3–9) were isolated from an extract of the Canadian medicinal plant Empetrum nigrum that significantly inhibited growth Mycobacterium tuberculosis H37Ra. The structures 1 2 established through analysis NMR MS data. antimycobacterial activity was attributed primarily to presence two chalcone derivatives (6 7) exhibited selective (IC50 values 23.8 32.8 μM, respectively) in comparison mammalian (HEK 293) cells 109...
DCAF16 is a substrate recognition component of Cullin-RING E3 ubiquitin ligases that can be targeted by electrophilic PROTACs (proteolysis targeting chimeras) to promote the nuclear-restricted degradation proteins. The endogenous protein substates remain unknown. In this study, we compared content DCAF16-wild type and DCAF16-knockout (KO) cells untargeted mass spectrometry-based proteomics, identifying Tudor domain-containing Spindlin-4 (SPIN4) as with level was substantially increased in...
Abstract Ferroptosis is an iron-dependent form of cell death driven by the oxidation polyunsaturated (PUFA) phospholipids. Large-scale genetic screens have pointed to a specialized role for PUFA ether phospholipids (ePLs) in promoting ferroptosis. Our understanding enzymes involved ePL production, however, remains incomplete. Here we show using combination pathway mining dependency maps, AlphaFold-guided structure predictions, and targeted lipidomics that uncharacterized transmembrane...
Summary Most human proteins lack chemical probes, and several large-scale generalizable small-molecule binding assays have been introduced to address this problem. How compounds discovered in such “binding-first” affect protein function, nonetheless, often remains unclear. Here, we describe a “function-first” proteomic strategy that uses size exclusion chromatography (SEC) assess the global impact of electrophilic on complexes cells. Integrating SEC data with cysteine-directed activity-based...
CDC25B belongs to the CDC25 family, and it plays an important part in regulating activity of CDK/CyclinA. Studies have shown that is closely related cancer development. When CYS473 on mutated into ASP, affinity between CDK2/CyclinA weakens, their dissociation speed greatly improved. However, mechanism by which CDC25BC473D mutant weakens its binding unclear. In order study effect mutants substrates, we constructed verified rationality CDC25BWT:CDK2/CyclinA system CDC25BC473D:CDK2/CyclinA...
When wars, major disasters, or epidemics of the infectious diseases occur, existing medical facilities are usually unable to implement timely and effective treatment for patients, reception capacity is difficult meet surge in demand health care. The makeshift emergency hospitals built patient reception, even isolation disease control. have developed improved modern times, including mobile field hospitals, tent navy hospital ships equipped with advanced equipment commonly used military...