A5067928868- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- CRISPR and Genetic Engineering
- Fish Ecology and Management Studies
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Reproductive biology and impacts on aquatic species
- Physiological and biochemical adaptations
- Genomics and Phylogenetic Studies
- Studies on Chitinases and Chitosanases
- Cancer-related molecular mechanisms research
- Gene expression and cancer classification
- Chromosomal and Genetic Variations
- Gut microbiota and health
- Birth, Development, and Health
- Insect symbiosis and bacterial influences
- Molecular Biology Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Adipose Tissue and Metabolism
- Fish Biology and Ecology Studies
- Lipid metabolism and biosynthesis
- Invertebrate Immune Response Mechanisms
- Protein Hydrolysis and Bioactive Peptides
- Probiotics and Fermented Foods
- RNA Interference and Gene Delivery
Norwegian University of Life Sciences
2017-2025
Novavax (United States)
2011
Atlantic salmon migrates from rivers to sea feed, grow and develop gonads before returning spawn in freshwater. The transition marine habitats is associated with dramatic changes the environment, including water salinity, exposure pathogens shift dietary lipid availability. Many physiology metabolism occur across this life-stage transition, but little known about molecular nature of these changes. Here, we use a long-term feeding experiment study transcriptional regulation gut liver both...
The anadromous Atlantic salmon undergo a preparatory physiological transformation before seawater entry, referred to as smoltification. Key molecular developmental processes involved in this life stage transition, such remodeling of gill functions, are known be synchronized and modulated by environmental cues like photoperiod. However, little is about the photoperiod influence genome regulatory driving other canonical aspects smoltification large-scale changes lipid metabolism energy...
In Atlantic salmon (Salmo salar), infectious anemia virus (ISAV) and pancreatic necrosis (IPNV) evade host immune response through complex antagonistic mechanisms. Type I interferons (IFNs) play a pivotal role in antiviral defense by signaling heterodimeric receptors to activate the JAK-STAT pathway drives expression of interferon-stimulated genes (ISGs). this study, CRISPR-Cas9 was used knock out (KO) interferon receptor (crfb1a, crfb5a, il10rb, ifngr2a) combined group candidate ifngr2a,...
Whole-genome duplications (WGD) and transposable element (TE) activity can act synergistically in genome evolution. WGDs increase TE directly through cellular stress or indirectly by relaxing selection against insertions functionally redundant, duplicated regions. Because TEs function as, evolve into, TE-derived cis -regulatory elements (TE-CREs), bursts of following WGD are therefore likely to impact evolution gene regulation. Yet, the role regulatory after is not well understood. Here we...
Characterization of essential genes across the genome is fundamental to understanding cellular functions at a molecular level. While significant progress has been made in characterizing human and mouse models, relatively little known about porcine genome. Pigs are an important production species now emerging as valuable models for studying diseases due their physiological similarities humans. To map genome, we have developed novel genome-wide CRISPR knockout screening library (pGeCKO)...
Domestication of animals imposes strong targeted selection for desired traits but can also result in unintended due to new domestic environments. Atlantic salmon (Salmo salmar) was domesticated the 1970s and has subsequently been selected faster growth systematic breeding programmes. More recently, aquaculture replaced fish oils (FOs) with vegetable (VOs) feed, radically changing levels essential long-chain polyunsaturated fatty acids (LC-PUFAs). Our aim here study impact domestication on...
The expansion of genomic resources for Atlantic salmon over the past half decade has enabled efficient interrogation genetic traits by large-scale correlation genotype to phenotype. Moving from causation will require genotype-phenotype relationships be tested experimentally in a cost-efficient and cell context-relevant manner. To enable such future experiments, we have developed method isolation manipulation primary hepatocytes use heterologous expression, reporter assay, gene editing...
Fillet fat content is an important production trait in Atlantic salmon (Salmo salar) because it influences the flavor, texture, and nutritional properties of product. Selection can be applied to alter muscle content, however how such selection changes underlying molecular physiology these animals unknown. Here, we examine link between genomic predicted breeding value for fillet 184 fish gene expression liver with emphasis on lipid metabolism genes. We found that higher values had genes...
Abstract Background One objective of this study was to identify putative quantitative trait loci (QTL) that affect indicator phenotypes for growth, nitrogen, and carbon metabolism in muscle, liver, adipose tissue, feed efficiency. Another perform an RNAseq analysis (184 fish from all families), genes are associated with nitrogen the liver. The material consisted a family experiment performed freshwater included 2281 individuals 23 full-sib families. During 12-day conversion test, families...
Salmon is an important source of long-chain highly unsaturated fatty acids (LC-HUFAs) such as 22:6n-3 [docosahexaenoic acid (DHA)]. In the present study, we conducted two identical experiments on salmon in freshwater (FW) and seawater (SW) stages, with a diet switch from fish oil (high LC-HUFA) to vegetable (low vice versa. Our aim was investigate life stage-specific features lipid uptake (gut), processing (liver), deposition (muscle). The composition changed much faster gut SW relative FW...
Hepatic lipid metabolism is traditionally investigated in vitro using hepatocyte monocultures lacking the complex three-dimensional structure and interacting cell types essential liver function. Precision cut slice (PCLS) culture represents an alternative system, which benefits from retention of tissue architecture. Here, we present first comprehensive evaluation PCLS method fish (Atlantic salmon, Salmo salar L.) validate it context feeding trials, extensive transcriptomic data, fatty acid...
Abstract Domestication of Salmo salar has imposed strong selection for production traits since the 1970s. The domestication also a radical shift in diet. Whereas wild salmon eats invertebrates, crustaceans and fish, dietary lipids domestic feed 1990 gradually shifted from fish oil (FO) to vegetable (VO), causing decrease intake long-chain polyunsaturated fatty acids (LC-PUFA). We tested hypothesis that this induced domestication-specific features lipid metabolism 96-day feeding trial...
ABSTRACT Background Chitin is a common component in the natural diet of many fish, and range chitinases with potential to down chitin have been identified. Yet whether metabolized fish still unclear. Here we used combination chitinase activity assay, transcriptomics, 16S rRNA bacterial analysis assess effect supplementation on Atlantic salmon gene expression microbial community. Results express multiple genes associated metabolism, show that are not affected by addition dietary chitin. We...
The expansion of genomic resources for Atlantic salmon over the past half decade has enabled efficient interrogation genetic traits by large-scale correlation genotype to phenotype. Moving from causation will require genotype-phenotype relationships be tested experimentally in a cost-efficient and cell context relevant manner. To enable such future experiments, we have developed method isolation manipulation primary hepatocytes use heterologous expression, reporter assay, gene editing...
Abstract Atlantic salmon migrates from rivers to sea feed, grow and develop gonads before returning spawn in freshwater. These habitat shifts require great phenotypic plasticity. To address the unresolved question of how shift diet between fresh saltwater affects regulation metabolic function, we fed contrasting diets each two life stages. Combining transcriptomics with comparative genomics, found that lipid metabolism undergoes a concerted fresh- Lipogenesis transport become less active...
Fatty acid desaturase 2 (Fads2) is the key enzyme of long chain polyunsaturated fatty (LC-PUFA) biosynthesis. Endogenous production these biomolecules in vertebrates, if present, insufficient to meet demand. Hence, LC-PUFA are considered as conditionally-essential. At present however, globally-limited nutrients due anthropogenic factors. Attention research given therefore find ways maximize endogenous especially species, whereby deeper knowledge on molecular mechanisms enzymatic steps...
Abstract The anadromous Atlantic salmon undergo a preparatory physiological transformation before seawater entry, referred to as smoltification. Key molecular developmental processes involved in this life stage transition, such remodeling of gill functions, are known be synchronized and modulated by environmental cues like photoperiod. However, little is about the photoperiod influence genome regulatory driving other canonical aspects smoltification large-scale changes lipid metabolism...
Abstract Muscle fat content is an important production trait in Atlantic salmon ( Salmo salar ) because it influences the flavor, texture, and nutritional properties of fillet. Genomic selection can be applied to alter muscle content, however how such changes underlying molecular physiology these animals unknown. Here, we examine link between genomic prediction by correlating breeding values for liver gene expression 184 fish. We found that Salmon with higher had genes lipid metabolism...
Abstract This study facilitates design of expression vectors and lentivirus tools for gene editing Atlantic salmon. We have characterized widely used heterologous promoters novel endogenous in salmon cells. qPCR to evaluate the activity several U6 sgRNA expression, including human (hU6), tilapia (tU6), mouse (mU6), zebrafish (zU6), (sU6), medaka (medU6), fugu (fU6) promoters. also evaluated polymerase type II (pol II) by luciferase assay. Our results showed that hU6 tU6 were most active...
ABSTRACT Microbiome-directed dietary interventions such as microbiota-directed fibers (MDFs) have a proven track record in eliciting responses beneficial gut microbes and are increasingly being promoted an effective strategy to improve animal production systems. Here we used initial metataxonomic data on fish microbiomes well wealth of priori mammalian microbiome knowledge α-MOS β-mannan-derived MDFs study effects feed supplements Atlantic salmon ( Salmo salar ) their hitherto poorly...
This study facilitates design of expression vectors and lentivirus tools for gene editing Atlantic salmon. We have characterized widely used heterologous promoters novel endogenous in salmon cells. qPCR to evaluate the activity several U6 sgRNA expression, including human (hU6), tilapia (tU6), mouse (mU6), zebrafish (zU6), (sU6), medaka (medU6), fugu (fU6) promoters. also evaluated polymerase type II (pol II) by luciferase assay. Our results showed that hU6 tU6 were most active among all...