A5064586896- Studies on Chitinases and Chitosanases
- Aquaculture disease management and microbiota
- Aquaculture Nutrition and Growth
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Chromosomal and Genetic Variations
- Genetic and phenotypic traits in livestock
- Protein Hydrolysis and Bioactive Peptides
- Enzyme Production and Characterization
- Invertebrate Immune Response Mechanisms
- Spectroscopy and Chemometric Analyses
- Advanced Chemical Sensor Technologies
- Thermography and Photoacoustic Techniques
- Genomic variations and chromosomal abnormalities
- Proteins in Food Systems
Norwegian University of Life Sciences
2015-2023
Structural variants (SVs) are a major source of genetic and phenotypic variation, but remain challenging to accurately type hence poorly characterized in most species. We present an approach for reliable SV discovery non-model species using whole genome sequencing report 15,483 high-confidence SVs 492 Atlantic salmon (Salmo salar L.) sampled from broad phylogeographic distribution. These recover population structure with high resolution, include active DNA transposon, widely affect...
Abstract Background Whole genome duplication (WGD) events have played a major role in eukaryotic evolution, but the consequence of these extreme adaptive evolution is still not well understood. To address this knowledge gap, we used comparative phylogenetic model and transcriptomic data from seven species to infer selection on gene expression duplicated genes (ohnologs) following salmonid WGD 80–100 million years ago. Results We find rare cases tissue-specific pervasive affecting many...
Microorganisms use a host of enzymes, including processive glycoside hydrolases, to deconstruct recalcitrant polysaccharides sugars. Processive hydrolases closely associate with polymer chains and repeatedly cleave glycosidic linkages without dissociating from the crystalline surface after each hydrolytic step; they are typically most abundant enzymes in both natural secretomes industrial cocktails by virtue their significant potential. The ubiquity aromatic residues lining enzyme catalytic...
The enzymatic degradation of recalcitrant polysaccharides is accomplished by synergistic enzyme cocktails glycoside hydrolases (GHs) and accessory enzymes. Many GHs are processive which means that they remain attached to the substrate in between subsequent hydrolytic reactions. Chitinases catalyze hydrolysis chitin (β-1,4-linked N-acetylglucosamine). Previously, a relationship active site topology processivity has been suggested while recent computational efforts have link degree ligand...
Abstract Chitin is one of the most abundant polysaccharides in nature, forming important structures insects, crustaceans, and fungal cell walls. Vertebrates on other hand are generally considered “nonchitinous” organisms, despite having highly conserved chitin metabolism-associated genes. Recent work has revealed that largest group vertebrates, teleosts, have potential to both synthesize degrade endogenous chitin. Yet, little known about genes proteins responsible for these dynamic...
Atlantic salmon (Salmo salar) possesses a genome containing 10 genes encoding chitinases, yet their functional roles remain poorly understood. In other fish species, chitinases have been primarily linked to digestion, but also functions, as chitinase-encoding are transcribed in variety of non-digestive organs. this study, we investigated the properties two belonging family 18 glycoside hydrolase group, namely Chia.3 and Chia.4, both isolated from stomach mucosa. exhibiting 95% sequence...
Abstract Whole genome duplication (WGD) events have played a major role in eukaryotic evolution, but the consequence of these extreme adaptive evolution is still not well understood. To address this knowledge gap we used comparative phylogenetic model and transcriptomic data from seven species to infer selection on gene expression duplicated genes (ohnologs) following salmonid WGD 80-100 million years ago. We find rare cases tissue-specific pervasive affecting many tissues, reflecting strong...
ABSTRACT Background Chitin is a common component in the natural diet of many fish, and range chitinases with potential to down chitin have been identified. Yet whether metabolized fish still unclear. Here we used combination chitinase activity assay, transcriptomics, 16S rRNA bacterial analysis assess effect supplementation on Atlantic salmon gene expression microbial community. Results express multiple genes associated metabolism, show that are not affected by addition dietary chitin. We...
Abstract Structural variants (SVs) are a major source of genetic and phenotypic variation, but remain challenging to accurately type hence poorly characterized in most species. We present an approach for reliable SV discovery non-model species using whole genome sequencing report 15,483 high-confidence SVs 492 Atlantic salmon ( Salmo salar L.) sampled from broad phylogeographic distribution. These recover population structure with high resolution, include active DNA transposon, widely affect...
ABSTRACT Background Chitin is one of the most abundant polysaccharides in nature, forming important structures insects, crustaceans, and fungal cell walls. Vertebrates on other hand are generally considered “non-chitinous” organisms, despite having highly conserved chitin metabolism associated genes. Recent work has revealed that largest group vertebrates, teleosts, have potential to both synthesize degrade endogenous chitin. Yet little still known about genes proteins responsible for these...
ABSTRACT The Atlantic salmon ( Salmo salar ) genome contains 10 chitinase encoding genes, but little is known about the function of these chitinases. We show that protein products three family 18 glycoside hydrolase (GH18) chitinases Chia.3, Chia.4, and Chia.7 are secreted in stomach mucosa amongst most abundant proteins this matrix. Chia.3 sharing 95% sequence identity, were not possible to separate by standard chromatographic methods thus purified as a pair. Biochemical analysis revealed...