A5041137669- Mosquito-borne diseases and control
- Malaria Research and Control
- Vector-borne infectious diseases
- Fish biology, ecology, and behavior
- Insect Resistance and Genetics
- Parasite Biology and Host Interactions
- Viral Infections and Vectors
- Identification and Quantification in Food
- Insect Pest Control Strategies
- Insect symbiosis and bacterial influences
- Zoonotic diseases and public health
- Conservation, Biodiversity, and Resource Management
- Aquaculture disease management and microbiota
- Insect and Arachnid Ecology and Behavior
- Species Distribution and Climate Change
- Urban Development and Societal Issues
- Oil Palm Production and Sustainability
- Viral Infectious Diseases and Gene Expression in Insects
- Aquaculture Nutrition and Growth
- Neurobiology and Insect Physiology Research
- Rural Development and Agriculture
- Agricultural and Food Sciences
- Entomopathogenic Microorganisms in Pest Control
- Vibrio bacteria research studies
- Amphibian and Reptile Biology
Instituto Pasteur
2022-2025
Secretaria da Saúde
2013-2023
Departamento de Ciência e Tecnologia
1988-2022
Walter Reed Army Institute of Research
1995
Malaria is a vector-borne disease that great burden on the poorest and most marginalized communities of tropical subtropical world. Approximately 41 species Anopheline mosquitoes can effectively spread Plasmodium parasites cause human malaria. Proposing natural classification for subfamily Anophelinae has been continuous effort, addressed using both morphology DNA sequence data. The monophyly genus Anopheles , phylogenetic placement Bironella subgenera Kerteszia Lophopodomyia Stethomyia...
Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) analysis was applied to samples from widespread populations of the poorly characterized Anopheles (Nyssorhynchus) albitarsis Lynch-Arribálzaga species complex, and 4 genetically differentiated were distinguished. A screen 65 random decamer oligonucleotide primers identified 12 primers, which produced 19 reproducible species-specific genetic markers common 2 or more species. These correlated in nearly all individuals each...
Specimens of neotropical Anopheles (Nyssorhynchus) were collected and identified morphologically. We amplified three genes for phylogenetic analysis–the single copy nuclear white CAD genes, the COI barcode region. Since we had multiple specimens most species able to test how well or combined corroborate morphologically defined by placing into exclusive groups. found that including region, poor at confirming species, but do so much better. This has implications identification, delimitation,...
The Coronator Group currently encompasses six morphologically similar species (Culex camposi Dyar, Culex coronator Dyar and Knab, covagarciai Forattini, usquatus usquatissimus ousqua Dyar). has been incriminated as a potential vector of West Nile Virus (WNV), Saint Louis Encephalitis (SLEV), Venezuelan Equine (VEEV). complete mitochondrial genome Cx. coronator, usquatus, Cx.usquatissimus, was sequenced, annotated, analyzed to provide genetic information about these species.The genomes varied...
The ability to successfully identify and incriminate pathogen vectors is fundamental effective control management. This task confounded by the existence of cryptic species complexes. Molecular markers can offer a highly means identification in such complexes are routinely employed study medical entomology. Here we evaluate multi-locus system for potential malaria Anopheles strodei subgroup.Larvae, pupae adult mosquitoes (n = 61) from An. subgroup were collected 21 localities nine Brazilian...
Malaria remains a significant public health issue in South America. Future climate change may influence the distribution of disease, which is dependent on those Anopheles mosquitoes competent to transmit Plasmodium falciparum. Herein, predictive niche models habitat suitability for P. falciparum, current primary vector darlingi and nine other known and/or potential species Neotropical Albitarsis Complex, were used document situation project future scenarios under changes America 2070.To...
Following yellow fever virus (YFV) isolation in monkeys from the São José do Rio Preto region and two fatal human autochthonous cases Ribeirão region, State of Paulo, Brazil, expeditions for entomological research eco-epidemiological evaluation were conducted.A total 577 samples humans, 108 3,049 mosquitoes analyzed by one or more methods: isolation, ELISA-IgM, RT-PCR, histopathology immunohistochemical.Of samples, 531 tested with 3 positives, 235 inoculated into mice 199 cell culture,...
The major drivers of the extensive biodiversity Neotropics are proposed to be geological and tectonic events together with Pliocene Pleistocene environmental climatic change. Geographical barriers represented by rivers Amazonas/Solimões, Andes coastal mountain ranges in eastern Brazil have been hypothesized lead diversification within primary malaria vector, Anopheles (Nyssorhynchus) darlingi Root, which primarily inhabits rainforest. To test this biogeographical hypothesis, we analyzed 786...
Brazilian malaria control programmes successfully reduced the incidence and mortality rates from 2005 to 2016. Since 2017, increased has been reported across Amazon. Few field studies focus on primary vector in high moderate endemic areas, Nyssorhynchus darlingi, as key entomological component of risk, metrics Plasmodium vivax propagation Amazonian rural communities. Human landing catch collections were carried out 36 houses 26 communities five municipalities states Acre, Amazonas Rondônia...
Inter-relationships among mosquito vectors, Plasmodium parasites, human ecology, and biotic abiotic factors, drive malaria risk. Specifically, rural landscapes shaped by activities have a great potential to increase the abundance of putting many vulnerable people at Understanding which point vectors increases in landscape can help design policies interventions for effective sustainable control. Using dataset adult female mosquitoes collected 79 sites endemic areas Brazilian Amazon, this...
We undertook geometric morphometric analysis of wing venation to assess this character's ability distinguish Anopheles darlingi Root populations and test the hypothesis that from coastal areas Brazilian Atlantic Forest differ those interior Forest, Cerrado, regions South North Amazon River. Results suggest are more similar each other than any regional populations. Notably, Cerrado population was north River collected south River, thus showing no correlation with geographical distances....
Abstract The relationship between deforestation and malaria is a spatiotemporal process of variation in Plasmodium incidence human-dominated Amazonian rural environments. present study aimed to assess the underlying mechanisms malarial exposure risk at fine scale 5-km 2 sites across Brazilian Amazon, using field-collected data with longitudinal spatiotemporally structured approach. Anopheline mosquitoes were sampled from 80 investigate infection rate mosquito communities estimate landscapes....
Deforestation in the Amazon and social vulnerability of its settler communities has been associated with increased malaria incidence. The feeding biology most important vectors region, notably Nyssorhynchus darlingi, compounds efforts to control reduce transmission what become known as "Frontier Malaria". Exploring Anophelinae mosquito diversity is fundamental understanding species responsible for developing appropriate management intervention strategies River basin.
ABSTRACT Malaria in South America remains a serious public health problem. Anopheles ( Nyssorhynchus ) darlingi is the most important malaria vector across tropical Latin America. Vector-targeted disease control efforts require thorough understanding of mosquito demographic and evolutionary patterns. We present analyze whole genomes 1094 A. (median depth 18x) from six American countries. observe deep geographic population structure, high genetic diversity including thirteen putative...
After detecting the death of Howlers monkeys (genus Alouatta) and isolation yellow fever virus (YFV) in Buri county, São Paulo, Brazil, an entomological research study field was started. A YFV strain isolated from newborn Swiss mice cultured cells Aedes albopictus - C6/36, a pool six Haemagogus (Conopostegus) leucocelaenus (Hg. leucocelaenus) mosquitoes (Dyar & Shannon) collected at site. Virus RNA fragment amplified by RT-PCR sequenced. The MCC Tree generated showed that is related to...
Abstract Background Environmental disturbance, deforestation and socioeconomic factors all affect malaria incidence in tropical subtropical endemic areas. Deforestation is the major driver of habitat loss fragmentation, which frequently leads to shifts composition, abundance spatial distribution vector species. The goals present study were to: (i) identify anophelines found naturally infected with Plasmodium ; (ii) measure effects landscape on number Nyssorhynchus darlingi , presence...
Yellow fever virus (YFV) is an endemic arbovirus in parts of Africa and the Americas. In Brazil, following eradication urban transmission cycle, YFV maintained a sylvatic cycle involving several species neotropical primates mosquitoes genera Haemagogus Sabethes, which serve as primary secondary vectors, respectively. During 2016–2019 outbreak São Paulo State, total 3,731 mosquito pools were collected from sites with ongoing epizootic events 192 municipalities. RT-qPCR...
Yellow fever virus (YFV) is an endemic arbovirus in parts of Africa and the Americas. In Brazil, following eradication urban transmission cycle, YFV maintained a sylvatic cycle involving several species neotropical primates mosquitoes genera Haemagogus Sabethes, which serve as primary secondary vectors, respectively. During 2016-2019 outbreak São Paulo State, total 3731 mosquito pools were collected from sites with ongoing epizootic events 192 municipalities. The RT-qPCR analysis detected 46...
Phylogenetic relationships among 21 species of mosquitoes in subgenus Nyssorhynchus were inferred from the nuclear white and mitochondrial NADH dehydrogenase subunit 6 (ND6) genes. Bayesian phylogenetic methods found that none three Sections within (Albimanus, Argyritarsis, Myzorhynchella) supported all analyses, although Myzorhynchella was to be monophyletic at combined Within Albimanus Section monophyly Strodei Subgroup strongly Anopheles antunesi An. lutzii formed a group. The...
The molecular phylogenetic relationships and population structure of the species Anopheles triannulatus complex: s.s., halophylus putative C were investigated. mitochondrial COI gene, nuclear white gene rDNA ITS2 samples that include known geographic distribution these taxa analyzed. Phylogenetic analyses performed using Bayesian inference, Maximum parsimony likelihood approaches. Each data set analyzed septely yielded a different topology but none provided evidence for seption An. C,...
Nucleotide sequences of the internal transcribed spacer 2 (ITS2) rDNA and partial cytochrome coxidase subunit I (COI) mtDNA white gene nDNA were obtained from specimens Anopheles nuneztovari A collected in Macapá (state Amapá), Óbidos, Prainha Almeirim Pará), Itacoatiara Parintins Amazonas), Brazil, compared with previously published A. s.l. Results Bayesian phylogenetic analyses performed using either COI or combined ITS2, suggest that An. B/C is distinct Amazonas/Solimões River basin....
Mitochondrial genome sequences are widely used as molecular markers for phylogenetic studies of mosquito species complexes, such the Anopheles albitarsis complex. Except a few that employed limited number nuclear or mitochondrial loci to address genetic structure and status cruzii , bellator homunculus little is known about can be in focusing on Kerteszia species. The complete genomes seven specimens An. An.cruzii laneanus were sequenced using long-range polymerase chain reaction Illumina...
The Anopheles albitarsis group of mosquitoes comprises eight recognized species and one mitochondrial lineage. Our knowledge malaria vectorial importance the distribution evolution these taxa is incomplete. We constructed ecological niche models (ENMs) for used hypothesized phylogenetic relationships ENMs to investigate environmental divergence associated with speciation events. Two major clades were identified, north (Clade 1) south 2) Amazon River that likely or was a barrier mosquito...
The subgenus Melanoconion of the mosquito genus Culex is taxonomically diverse and widely distributed in Neotropical Region, with 10 species occurring Nearctic Region. Species this pose a taxonomical challenge because morphological identification based largely on anatomical characters male genitalia. We addressed monophyly Spissipes Sections some informal groups each section. Our sample taxa included 97 specimens representing 43 species, from which we analysed fragments two single-copy...