A5078340621- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Wnt/β-catenin signaling in development and cancer
- Selenium in Biological Systems
- Congenital heart defects research
- Histone Deacetylase Inhibitors Research
- Hippo pathway signaling and YAP/TAZ
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Autophagy in Disease and Therapy
- Developmental Biology and Gene Regulation
- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- Protein Degradation and Inhibitors
- ATP Synthase and ATPases Research
- Trace Elements in Health
- Lipid metabolism and biosynthesis
- Ferroptosis and cancer prognosis
- Signaling Pathways in Disease
- RNA Research and Splicing
- Polyamine Metabolism and Applications
- Metabolism and Genetic Disorders
- Bacterial Genetics and Biotechnology
Fudan University
2009-2025
Huashan Hospital
2025
Zhongshan Hospital
2018-2024
Guangzhou University
2024
Fudan University Shanghai Cancer Center
2021-2024
Shanghai Medical College of Fudan University
2023-2024
Shanghai Cancer Institute
2023-2024
First Hospital of China Medical University
2024
China Medical University
2024
Center for Life Sciences
2012-2023
Significance Upstream cell death stimuli culminate in the activation of an initiator caspase, marking onset apoptosis. Activation caspase-9, is mediated by heptameric Apaf-1 apoptosome. How apoptosome facilitates autocatalytic caspase-9 has remained controversial and largely enigmatic. Two contrasting but not mutually exclusive hypotheses, proximity-induced dimerization vs. induced conformation, emphasize different aspects caspase activation. This study provides compelling evidence to...
AMOT (angiomotin) is a membrane-associated protein that expressed in ECs (endothelial cells) and controls migration, TJ (tight junction) formation, cell polarity angiogenesis. Recent studies have revealed two AMOT-like proteins, AMOTL1 AMOTL2, play critical roles the Hippo pathway by regulating subcellular localization of co-activators YAP (Yes-associated protein) TAZ (transcriptional co-activator with PDZ-binding motif). However, it has been unclear how regulated. In present study, we...
The maintenance of a constant ATP level (‘set-point’) is vital homeostatic function shared by eukaryotic cells. In particular, mammalian myocardium exquisitely safeguards its set-point despite 10-fold fluctuations in cardiac workload. However, the exact mechanisms underlying this regulation homeostasis remain elusive. Here we show mitochondrial flashes (mitoflashes), recently discovered dynamic activity mitochondria, play an essential role for auto-regulation heart. Specifically, mitoflashes...
Research Article9 March 2018Open Access Source DataTransparent process Dual inhibition of AKT-mTOR and AR signaling by targeting HDAC3 in PTEN- or SPOP-mutated prostate cancer Yuqian Yan Department Gastroenterology, Jiangxi Institute Gastroenterology Hepatology, First Affiliated Hospital Nanchang University, Nanchang, Jiangxi, China Biochemistry Molecular Biology, Mayo Clinic College Medicine, Rochester, MN, USA Search for more papers this author Jian An Yinhui Yang Urology, The Fourth...
Considering the key role of myeloid cell differentiation—related genes in tumor microenvironment (TME), we aimed to build a prognostic risk model using these for Lung adenocarcinoma (LUAD). The mRNA gene expression profiles LUAD patients from Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were downloaded as training validation sets. Then, "edgeR" R package was applied screen out differentially expressed (DEGs) univariate cox regression, backward stepwise selection...
Because of its stringent sequence specificity, tobacco etch virus (TEV) protease is widely used to remove fusion tags from recombinant proteins. Due the poor solubility TEV protease, many strategies have been employed increase expression level this enzyme. In our work, we introduced a novel method produce by using visible superfolder green fluorescent protein ( sf GFP) as tag. The soluble production and catalytic activity six variants GFP‐TEV was examined, then best variant selected for...
Acquisition of resistance to phosphatidylinositol 3-kinase (PI3K)/AKT-targeted monotherapy implies the existence common mechanisms independent cancer type. Here, we demonstrate that PI3K/AKT inhibitors cause glycolytic crisis, acetyl-coenzyme A (CoA) shortage, and a global decrease in histone acetylation. In addition, induce drug by selectively augmenting H3 lysine 27 acetylation (H3K27ac) binding CBP/p300 BRD4 proteins at subset growth factor receptor (GF/R) gene loci. occupation these loci...
Hepatic steatosis, a hallmark of metabolic dysfunction-associated steatotic liver disease (MASLD), arises from disrupted lipid homeostasis. Mitochondrial dysfunction, particularly imbalances in mitochondrial fusion and fission, plays crucial role MASLD progression. Irisin, an exercise-induced myokine, is involved metabolism, though its precise mechanisms action remain unclear. An irisin-Fc protein was prophylactically administered to mice with high-fat diet (HFD)-induced for 12 weeks. Liver...
Tropomyosin (Tpm) proteins, encoded by four Tpm genes (Tpm1–4), are associated with the stabilization of F-actin filaments and play important roles in modulating muscle contraction. So far, little is known about Tpm4 function embryonic heart development its involvement cardiovascular diseases. In this study, we investigated functions different isoforms tpm4 heartbeat zebrafish. The transgenic zebrafish line, T2EGEZ8, was generated insertion a Tol2 transposon gene trap vector, homozygous...
Interplay between cell polarity module Scribble‐Lethal Giant Larvae‐Discs Large 1 (DLG1) and Yes‐associated protein (YAP) appears critical in tumor metastasis. We identified zinc finger 191 (ZNF191) as a metastasis suppressor acting through DLG‐YAP crosstalk hepatocellular carcinoma (HCC). Overexpression of ZNF191 HCC cells impaired motility, while depletion promoted migration vitro vivo triggering YAP signaling. Chromatin immunoprecipitation‐sequencing revealed that specifically bound to...
Glycoproteins have become the most often screened tumor markers in vitro diagnostics. Although a large number of electrochemical methods been proposed to sensitively detect glycoproteins, them involve aid laborious signal amplification. Herein, we report use glycan-evocated metallization (GlyMetal) for amplification-free detection glycoproteins at low concentration levels. Briefly, interest are captured by an aptamer recognition layer, and then glycans targets oxidized NaIO
FK506 binding protein 23 from mouse (mFKBP23) is a peptidyl‐prolyl cis ‐ trans isomerase (PPIase) the endoplasmic reticulum (ER), which consists of an N‐terminal PPIase domain and C‐terminal with Ca 2+ sites. The assay adsorption ER extract glutathione S ‐transferase‐mFKBP23 attached to glutathione‐Sepharose 4B shows that mFKBP23 binds immunoglobulin (mBiP). same recombinant proteins N‐ C‐termini C‐terminus ‐dependent switch point between 2 3 mM. By high concentration this cannot be...
Inhibition of histone deacetylases causes rapid and robust acetylation histones. In this case, is likely increased on nearly every nucleosome, the per-cell DNA/chromatin yield in chromatin immunoprecipitation (ChIP) experiments significantly increased. Spike-in controls are essential for normalizing ChIP sequencing (ChIP-seq) data to capture massive effect. Here, we report a detailed protocol H3K27-ac ChIP-seq human cells with from an ancestral species as spike-in control. For complete...
Hyperactivation of the oncogenic transcription reflects epigenetic plasticity cancer cells. Su(var)3-9, enhancer zeste, Trithorax (SET) was described as a nuclear factor that stimulated from chromatin template. However, mechanisms SET-dependent are unknown. Here, we found overexpression SET and CDK9 induced very similar transcriptome signatures in multiple cell lines. localized start site (TSS)–proximal regions supported RNA transcription. specifically bound PP2A-C subunit PP2A-A repulsion C...