A5100344599- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Ferroptosis and cancer prognosis
- Immune cells in cancer
- Plant Stress Responses and Tolerance
- Metalloenzymes and iron-sulfur proteins
- Drug Transport and Resistance Mechanisms
- Galectins and Cancer Biology
- Plant Molecular Biology Research
- Porphyrin and Phthalocyanine Chemistry
- MicroRNA in disease regulation
- Inflammatory Bowel Disease
Shanghai Jiao Tong University
2025
Shanghai First People's Hospital
2025
Qinghai University
2024
Hybrid Rapeseed Research Center of Shaanxi Province
2024
Guilin Medical University
2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2023
Chongqing Medical University
2023
Southern Medical University
2022
Nanfang Hospital
2022
Key Laboratory of Guangdong Province
2022
Metabolic switching during heart development contributes to postnatal cardiomyocyte (CM) cell cycle exit and loss of regenerative capacity in the mammalian heart. control has potential for developing effective CM proliferation strategies. We sought determine whether lactate dehydrogenase A (LDHA) regulated by inducing metabolic reprogramming.LDHA expression was high P1 hearts significantly decreased development. CM-specific LDHA knockout mice were generated using CRISPR/Cas9 technology....
Bone resorption relies on the extracellular acidification function of V-ATPase (vacuolar-type proton-translocating ATPase) proton pump(s) present in plasma membrane osteoclasts. The exact configuration osteoclast-specific ruffled border V-ATPases remains largely unknown. In study, we found that subunit Atp6v1c1 (C1) is highly expressed osteoclasts, whereas subunits Atp6v1c2a (C2a) and Atp6v1c2b (C2b) are not. expression level C1 induced by RANKL [receptor activator for NF-kappaB (nuclear...
It is known that V-ATPases (vacuolar H+-ATPase) are involved in breast cancer growth and metastasis. Part of this action similar to their role osteoclasts, where they're extracellular acidification matrix destruction; however, the roles subunits cell proliferation, signaling, other pro-tumor actions not well established. Analysis TCGA data shows V-ATPase subunit Atp6v1c1 overexpressed or amplified 34% human cases, with a 2-fold decrease survival at 12 years. Whereas subunits, such as...
Previous studies have shown that Atp6v1c1, a regulator of the assembly V0 and V1 domains V-ATPase complex, is up-regulated in metastatic oral tumors.Despite these studies, function Atp6v1c1 tumor growth metastasis still unknown.Atp6v1c1's expression squamous cell carcinoma indicates has an important cancer metastasis.We hypothesized elevated essential to promotes through activation activity.To test this hypothesis, Lentivirus-mediated RNAi knockdown approach was used study mouse 4T1 mammary...
Crohn's disease (CD) is a chronic inflammatory bowel with an unknown etiology. Ubiquitination plays significant role in the pathogenesis of CD. This study aimed to explore functional roles ubiquitination-related genes Differentially expressed were identified by intersecting differentially (DEGs) from GSE95095 dataset Gene Expression Omnibus (GEO) database set genes. Ontology (GO) and Kyoto Encyclopedia Genes Genomes (KEGG) analyses performed. Key selected combining hub protein-protein...
<title>Abstract</title> Background <bold>Halofantrine</bold> is a drug used to treat malaria, and recent studies have shown that it has potential glioblastoma. Objective To study the inhibitory effect of Halofantrine on glioblastoma its mechanism. Methods Based GEO database clinical samples, expression difference ATP6V0D2 gene in was detected. The U251 cells autophagy protein at levels were detected vitro. importance verified by constructing stable overexpression model cells. mechanism...
Background: The BBR-BPC gene family is a relatively conservative group of transcription factors, playing key role in plant morphogenesis, organ development, and responses to abiotic stress. Brassica napus L. (B. napus), commonly known as oilseed rape, an allopolyploid formed by the hybridization polyploidization rapa rapa) oleracea oleracea), one most important oil crops. However, little about characteristics, conservation, expression patterns this B. napus, especially under Methods: To...
Site-directed mutations D262C, D262H, D262N, and D262T were made to the β subunit Walker Homology B aspartate of chloroplast F1-ATPase in Chlamydomonas. Photoautotrophic growth photophosphorylation rates 3–14% wild type as ATPase activities purified F1 indicating that βD262 is an essential residue for catalysis. The EPR spectrum vanadyl bound Site 3 VO2+-ATP gave rise two species designated C mutants. 51V-hyperfine parameters C, present exclusively activated enzyme state, did not change...
Metal ligands of the VO(2+)-adenosine diphosphate (ADP) complex bound to high-affinity catalytic site 1 chloroplast F(1) adenosine triphosphatase (CF(1) ATPase) were characterized by electron paramagnetic resonance (EPR) spectroscopy. This EPR spectrum contains two species designated E and F not observed when VO(2+)-nucleotide is 3 CF(1). Site-directed mutations betaE197C, betaE197D, betaE197S in Chlamydomonas CF(1) impair ATP synthase ATPase activity catalyzed CF(1)F(o) soluble CF(1),...
Site-directed mutants Y317C, Y317E, Y317F, Y317G, and Y317K were made to the catch-loop tyrosine on beta subunit of chloroplast F(1)-ATPase in Chlamydomonas. EPR spectra VO(2+)-ATP bound site 3 CF(1) from wild type obtained. Every mutant changed (51)V hyperfine parameters VO(2+) at this catalytically active conformation enzyme but had no effect these form that predominates when activity is latent. These results indicate residue a ligand metal Mg(2+)-nucleotide complex binds empty catalytic...