ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMicrostructure of bacterially synthesized poly(3-hydroxybutyrate-co-3-hydroxyvalerate)Naoko Kamiya, Yasuhiko Yamamoto, Yoshio Inoue, Riichiro Chujo, and Yoshiharu DoiCite this: Macromolecules 1989, 22, 4, 1676–1682Publication Date (Print):April 1, 1989Publication History Published online1 May 2002Published inissue 1 April 1989https://pubs.acs.org/doi/10.1021/ma00194a030https://doi.org/10.1021/ma00194a030research-articleACS PublicationsRequest reuse...

The lipid scavenger receptor cluster of differentiation 36 (CD36) has been shown to have a pro-metastatic function in several cancers. Adipose tissue, favorable site for peritoneal metastasis (PM) from gastric cancer (GC), promotes this process by providing free fatty acids (FFAs); however, the role CD36 PM progression GC remains be elucidated.We evaluated expression cells under various conditions. overexpressing (CD36OE) MKN45 were prepared and their migration invasive properties assessed....

Abstract Cyclodextrins1,2 (CDs) are cyclic oligosaccharides composed of six or more (1→4) linked α-D-glucopyranosyl residues (see FIG. 1). The common CDs called α-, β-, and γ-CD constructed six, seven, eight units, respectively. CD molecule is a toroidal, hollow, truncated cone with primary secondary hydroxyl groups crowning the narrower wider rims, Methine protons glucosidic oxygens oriented in its interior space inside cavity, relatively hydrophobic. internal diameter cavity about 5∼9 Å for CDs.

Synchrotron radiation enables the observation of solidification in metallic alloys. In situ observations for Al–Cu alloys (5, 10 and 15 wt-%Cu) are reported. Nucleation fragmentation dendrite arms were often observed 10%Cu when unidirectional was performed from planar interface. contrast, nucleation rarely 5%Cu The ahead solidifying front mushy region strongly depended on alloy composition. This paper also presents Fe–10Si–0·5Al (at-%) dendritic growth δ-Fe clearly using this technique....

The structure of a complex between heme(Fe3+) and parallel G-quadruplex DNA formed from single repeat sequence the human telomere, d(TTAGGG), has been characterized by 1H NMR. study demonstrated that is sandwiched 3′-terminal G-quartets DNA. Hence, net +1 charge in surrounded eight carbonyl oxygen atoms G-quartets. Interaction heme Fe3+ was clearly manifested solvent 1H/2H isotope effect on NMR parameters paramagnetically shifted methyl proton signals, interaction with two shown to provide...

Until now, RNA G-quadruplexes were believed to only adopt a parallel G-quadruplex structure. In this study, we describe the first observation of an antiparallel formed by human telomere RNA. This newly described topology is great interest as it shows that can also be polymorphic and structures are different from configuration.

Parallel G-quadruplexes formed from oligonucleotide sequences, d(TTAGn), where n = 3−5, have been shown to form a dimer through end-to-end stacking of 3'-terminal G-tetrads. The monomers and dimers the are in dynamic equilibrium with an exchange rate ∼1 s-1. A thermodynamic study demonstrated that dimerization is largely enthalpic origin.

A water-soluble cationic porphyrin, 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)-21H,23H-porphyrin (TmPyP4), has been studied extensively because of its unique physicochemical properties that lead to interactions with nucleic acids, as well therapeutic application. Formation a complex between TmPyP4 and parallel G-quadruplex DNA formed from single repeat sequence the human telomere, d(TTAGGG), characterized in an effort elucidate mode molecular recognition DNA. The study demonstrated...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProton NMR characterization of metastable and equilibrium heme orientational heterogeneity in reconstituted native human hemoglobinGerd N. La Mar, Yasuhiko Yamamoto, Thomas Jue, Kevin M. Smith, Ravindra K. PandeyCite this: Biochemistry 1985, 24, 15, 3826–3831Publication Date (Print):July 1, 1985Publication History Published online1 May 2002Published inissue 1 July...

AbstractAbstractThis paper presents recent progress of in situ observation for the microstructure evolution during solidification. Nucleation and fragmentation dendrite arms are important issues controlling However, there few studies on nucleation metallic alloys. Time resolved X-ray imaging technique has been developed to observe solidification alloy systems situ. Fragmentation often occurred at root after growth velocity was reduced Sn–13 at.-%Bi alloys Al–15 mass%Cu In alloys, both...

Functional regulation of myoglobin (Mb) is thought to be achieved through the heme environment furnished by nearby amino acid residues, and subtle tuning intrinsic Fe reactivity. We have performed substitution strongly electron-withdrawing perfluoromethyl (CF(3)) group(s) as side chain(s) Mb obtain large alterations electronic structure in order elucidate relationship between O(2) affinity properties peripheral chains. utilized equilibrium constant (pK(a)) "acid-alkaline transition"...

Abstract The molecular structure and heme electronic of a complex (heme–(d(TTAGGGT))4 complex) between parallel G-quadruplex DNA formed from heptanucleotide d(TTAGGGT), i.e., (d(TTAGGGT))4, have been characterized using 1H NMR. study demonstrated that the is accommodated G6 T7 to form 1:1 DNA. spin state Fe3+ heme(Fe3+)–(d(TTAGGGT))4 exhibited characteristic pH-dependent change high (HS) state, S = 5/2, at low pH thermal equilibrium HS (LS), 1/2, pH, with midpoint value 8.9 ± 0.2. was...

Heme {Fe(II)- or Fe(III)-protoporphyrin IX complex [heme(Fe2+) heme(Fe3+), respectively]} binds selectively to the 3′-terminal G-quartet of a parallel G-quadruplex DNA formed from single repeat sequence human telomere, d(TTAGGG), through π–π stacking interaction between porphyrin moiety heme and G-quartet. The binding affinities some chemically modified hemes(Fe3+) for structures complexes hemes(Fe2+) DNA, with carbon monoxide (CO) coordinated Fe atom on side opposite G6 G-quartet, have been...

Heme methyl 1H and 13C resonances of met-cyano form myoglobin from the shark, Geleorhinus japonicus (GJMbCN), have been assigned via 1H-13C heteronuclear shift correlated spectroscopy (COSY) connectivities their hyperfine shifts were compared with those corresponding some hemoproteins. Variation heme pattern correlates well angle (phi) between projection proximal histidyl imidazole plane onto NII-Fe-NIV vector. The alteration interaction peripheral side-chains and/or iron-bound ligand...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProton NMR study of dynamics and thermodynamics heme rotational disorder in native reconstituted hemoglobin AYasuhiko Yamamoto Gerd N. La MarCite this: Biochemistry 1986, 25, 18, 5288–5297Publication Date (Print):September 1, 1986Publication History Published online1 May 2002Published inissue 1 September 1986https://pubs.acs.org/doi/10.1021/bi00366a045https://doi.org/10.1021/bi00366a045research-articleACS PublicationsRequest reuse...

Heme binds selectively to the 3′-terminal G-quartet (G6 G-quartet) of an all parallel-stranded tetrameric G-quadruplex DNA, [d(TTAGGG)]4, form a heme–DNA complex. Complexes between [d(TTAGGG)]4 and series chemically modified hemes possessing heme Fe atom with variety electron densities were characterized in terms their peroxidase activities evaluate effect change density (ρFe) on activities. The activity complex decreased decreasing ρFe, supporting idea that is elicited through reaction...

Heme in its ferrous and ferric states [heme(Fe2+) heme(Fe3+), respectively] binds selectively to the 3′-terminal G-quartet of all parallel-stranded monomeric G-quadruplex DNAs formed from inosine(I)-containing sequences, i.e., d(TAGGGTGGGTTGGGTGIG) DNA(18mer) d(TAGGGTGGGTTGGGTGIGA) DNA(18mer/A), through a π–π stacking interaction between porphyrin moiety heme G-quartet, form 1:1 complexes [heme–DNA(18mer) heme–DNA(18mer/A) complexes, respectively]. These exhibited enhanced peroxidase...

Ticks are notorious blood-sucking ectoparasites that affect both humans and animals. They serve as a unique vector of various deadly diseases. Here, we have shown the roles receptor for advanced glycation end products (RAGE) during repeated infestations by tick Haemaphysalis longicornis using RAGE

<div class="section abstract"><div class="htmlview paragraph">Improvement and evolution of all aircraft technologies the commercialization new are essential to carbon-net-zero goal air mobility. Passenger required provide ultimate in comfort, economy, safety, gas turbine engines will not disappear, while promoting conversion SAF hydrogen fuels. The More Electric Engine (or MEE) concept, which has been proposed since late 2000s, is one alternative. This paper focuses on...

The complete thermal-unfolding profiles of both oxidized and reduced forms cytochrome c551 (PA) from mesophilic Pseudomonas aeruginosa c552 (HT) thermophilic Hydrogenobacter thermophilus were obtained by the newly developed pressure-proof cell compartment installed in a circular dichroic spectrometer, which facilitates protein experiments up to 180 °C. thermodynamic cycle, relates stability redox function, indicated that potentials PA HT native state are regulated proteins rather than ones.