A5000074630- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Bladder and Urothelial Cancer Treatments
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Multiple Myeloma Research and Treatments
- Cancer Immunotherapy and Biomarkers
- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- Ferroptosis and cancer prognosis
- DNA Repair Mechanisms
- Immune Cell Function and Interaction
- Bone Metabolism and Diseases
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Genomics and Chromatin Dynamics
- Chronic Lymphocytic Leukemia Research
- Blood groups and transfusion
- Metabolism, Diabetes, and Cancer
- Neuropeptides and Animal Physiology
- Skin and Cellular Biology Research
- Carbohydrate Chemistry and Synthesis
- Peptidase Inhibition and Analysis
- Esophageal Cancer Research and Treatment
- Amyloidosis: Diagnosis, Treatment, Outcomes
Augusta University
2016-2025
Augusta University Health
2015-2025
National Cancer Center of Georgia
2019
Mansoura University
2019
Georgia Regents Medical Center
2013-2016
Clinical Research Management
2006
Princeton University
1994-1996
Yale University
1988-1994
A novel family of genes, characterized by the presence a region homology to DNA-binding domain Brachyury (T) locus product, has recently been identified. The named T-box, and new mouse genes that contain T-box have 1–6 (Tbx1 through Tbx6). As basis for further study function evolution these we examined expression 5 Tbx1–Tbx5, across wide range embryonic stages from blastocyst gastrulation early organogenesis in situ hybridization wholemounts tissue sections. Tbx3 is expressed earliest, inner...
Abstract CD73, an ecto-5′-nucleotidase (NT5E), serves as immune checkpoint by generating adenosine (ADO), which suppresses activation through the A 2A receptor. Elevated CD73 levels in tumor tissues correlate with poor clinical outcomes. However, crucial source of activity within microenvironment remains unspecified. Here, we demonstrate that cancer-associated fibroblasts (CAFs) constitute prominent hi population human colorectal cancers (CRCs) and two − murine models, including a modified...
Glucose-dependent insulinotropic peptide (GIP) is a 42-amino acid synthesized and secreted from endocrine cells in the small intestine. The role of GIP coupling nutrient intake insulin secretion, incretin effect, well known. We report that receptor messenger RNA protein are present normal bone osteoblast-like cell lines, high affinity receptors for can be demonstrated by [125I]GIP binding studies. When applied to (SaOS2), stimulated increases cellular cAMP content intracellular calcium, with...
Acute nutrient ingestion leads to a rapid inhibition of bone resorption while effects on makers formation are less marked or absent, suggesting that there is transient shift toward skeletal accretion in the immediate postprandial period. The cellular bases for these not clear. Glucose-dependent insulinotropic peptide (GIP), known modulator glucose-induced insulin secretion, secreted from intestinal endocrine cells response ingestion. In addition effect GIP pancreatic beta-cells, receptors...
Abstract The T-box genes comprise an ancient family of putative transcription factors conserved across species as divergent Mus musculus and Caenorhabditis elegans. All gene products are characterized by a novel 174-186amino acid DNA binding domain called the that was first discovered in polypeptide mouse T locus Drosophila melanogaster optomotor-blind gene. Earlier studies allowed identification five genes, T, Tbx1-3, Tbr1, all map to different chromosomal locations expressed unique...
Primary aldosteronism is a heterogeneous disease that includes both sporadic and familial forms. A point mutation in the KCNJ5 gene responsible for hyperaldosteronism type III. Somatic mutations also occur aldosterone producing adenomas (APA). The objective of study was to define effect on expression production using APA tissue human adrenocortical cells. microarray analysis used compare transcriptome profiles female-derived samples with without HAC15 adrenal cells overexpressing either...
Abstract Purpose: The perturbation of metabolic pathways in high-grade bladder cancer has not been investigated. We aimed to identify a signature by integrating unbiased metabolomics, lipidomics, and transcriptomics predict patient survival discover novel therapeutic targets. Experimental Design: performed high-resolution liquid chromatography mass spectrometry (LC-MS) bioinformatic analysis determine the global metabolome lipidome cancer. further investigated effects impaired using vitro...
Abstract Although clinically apparent metastasis is associated with late stages of cancer development, micro-metastatic dissemination may be an early event. However, the fate these disseminated tumor cells (DTC) remains elusive. We show that despite their capacity to disseminate into secondary organs, 4T1 models develop overt while EMT6-tumor bearing mice clear DTCs shed from primary tumors as well those introduced by intravenous (IV) injection. Following surgical resection EMT6 tumors, do...
Caveolae are 50-100 nm, nonclathrin-coated, flask-shaped plasma membrane microdomains that have been identified in most mammalian cell types, except lymphocytes and neurons. To date, multiple functions ascribed to caveolae, including the compartmentalization of lipid protein components function transmembrane signaling events, biosynthetic transport functions, endocytosis, potocytosis, transcytosis. Caveolin, a 21-24 kDa integral protein, is principal structural component caveolae. We...
Background African Americans (AAs) experience a disproportionally high rate of bladder cancer (BLCA) deaths even though their incidence rates are lower than those other patient groups. Using metabolomics approach, this study investigated how AA BLCA may differ molecularly from European (EAs) BLCA, and it examined serum samples patients with the aim identifying druggable metabolic pathways in patients. Methods Targeted was applied to measure more 300 metabolites 2 independent cohorts EA...
BACKGROUND: Glioblastoma (GBM) was shown to relapse faster and displayed therapeutic resistance antiangiogenic therapies (AATs) through an alternative tumor cell-driven mechanism of neovascularization called vascular mimicry (VM). We identified highly upregulated interleukin 8 (IL-8)-CXCR2 axis in cells high-grade human glioma AAT-treated orthotopic GBM tumors. METHODS: Human tissue sections array were used ascertain the clinical relevance CXCR2-positive formation VM. utilized U251 U87...
O-GalNAc glycans (or mucin O-glycans) play pivotal roles in diverse biological and pathological processes, including tumor growth progression. Structurally defined are essential for functional studies but synthetic challenges their inherent structural diversity complexity have limited access to these compounds. Herein, we report an efficient robust chemoenzymatic modular assembly (CEMA) strategy construct structurally glycans. The key this is the convergent of cores 1-4 6 from three chemical...
Activation of phosphatidylcholine-specific phospholipase D (PLD) occurs as part the complex signal-transduction cascade initiated by agonist stimulation tyrosine kinase and G-protein-coupled receptors. A variety mammalian PLD activities have been described, cDNAs for two PLDs recently reported (human PLD1 murine PLD2). We describe here cloning chromosomal localization PLD1. Northern-blot hybridization RNase protection analyses were used to examine expression PLD2 in a cell lines tissues....
Abstract Background: The current system to predict the outcome of smokers with bladder cancer is insufficient due complex genomic and transcriptomic heterogeneities. This study aims identify serum metabolite-associated genes related survival in this population. Methods: We performed LC/MS-based targeted metabolomic analysis for >300 metabolites obtained from two independent cohorts never smokers, healthy smokers. A subset differential was validated using Biocrates absoluteIDQ p180...
No AccessJournal of UrologyAdult Urology1 Jan 2020Clinical Parameters Outperform Molecular Subtypes for Predicting Outcome in Bladder Cancer: Results from Multiple Cohorts, Including TCGA Daley S. Morera, Sarrah L. Hasanali, Daniel Belew, Santu Ghosh, Zachary Klaassen, Andre R. Jordan, Jiaojiao Wang, Martha K. Terris, Roni J. Bollag, Axel Merseburger, Arnulf Stenzl, Mark Soloway, and Vinata B. Lokeshwar MoreraDaley Morera Department Biochemistry Biology, Medical College Georgia, Augusta...
Bladder cancer (BLCA) mortality is higher in African American (AA) patients compared with European (EA) patients, but the molecular mechanism underlying race-specific differences are unknown. To address this gap, we conducted comprehensive RNA-Seq, proteomics, and metabolomics analysis of BLCA tumors from AA EA. Our findings reveal a distinct metabolic phenotype characterized by elevated mitochondrial oxidative phosphorylation (OXPHOS), particularly through activation complex I. The results...