A5082674992- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Circular RNAs in diseases
- Inflammasome and immune disorders
- Ovarian cancer diagnosis and treatment
- interferon and immune responses
- Virus-based gene therapy research
- Dendrimers and Hyperbranched Polymers
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Gut microbiota and health
- IL-33, ST2, and ILC Pathways
- SARS-CoV-2 and COVID-19 Research
- Diabetes and associated disorders
- CRISPR and Genetic Engineering
- Immune cells in cancer
- Lymphoma Diagnosis and Treatment
- vaccines and immunoinformatics approaches
- Animal Virus Infections Studies
- Asthma and respiratory diseases
Inserm
2016-2024
Nantes Université
2016-2024
Centre National de la Recherche Scientifique
2016-2024
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers
2016-2021
Université d'Angers
2016-2020
Structure Fédérative de Recherche Bonamy
2020
Institut de Transplantation Urologie en Nephrologie
2018
Abstract MicroRNAs (miRNA), small noncoding RNAs that regulate gene expression, exist not only in cells but also a variety of body fluids. These circulating miRNAs could enable intercellular communication. are packaged membrane-encapsulated vesicles, such as exosomes, or protected by RNA-binding proteins. Here, we report included human melanoma exosomes the tumor immune response. Using microscopy and flow cytometry, demonstrate CD8+ T internalize from different types even if these do...
Background Clinical benefit from programmed cell death 1 receptor (PD-1) inhibitors relies on reinvigoration of endogenous antitumor immunity. Nonetheless, robust immunological markers, based circulating immune subsets associated with therapeutic efficacy are yet to be validated. Methods We isolated peripheral blood mononuclear three independent cohorts melanoma and Merkel carcinoma patients treated PD-1 inhibitor, at baseline longitudinally after therapy. Using multiparameter flow cytometry...
Recently, the inhibitory CD94/NKG2A receptor has joined group of immune checkpoints (ICs) and its expression been documented in NK cells CD8+ T lymphocytes several cancers some infectious diseases. In colorectal cancer (CRC), we previously reported that NKG2A+ tumor-infiltrating (TILs) are predominantly αβ CD94 overexpression and/or ligand HLA-E were associated with a poor prognosis. This study aimed to thoroughly characterize TIL subpopulation document impact NKG2A on anti-tumor responses...
Immune response against human cytomegalovirus (HCMV) includes a set of persistent cytotoxic NK and CD8 T cells devoted to eliminate infected prevent reactivation. HCMV antigens (pp65, IE1) presented by HLA class-I molecules are well characterized they associate with efficient virus control. HLA-E-restricted targeting UL40 signal peptides (HLA-EUL40) have recently emerged as non-conventional T-cell also observed in some hosts. The occurrence, specificity features HLA-EUL40 responses remain...
Attenuated measles virus (MV) exerts its oncolytic activity in malignant pleural mesothelioma (MPM) cells that lack type-I interferon (IFN-I) production or responsiveness. However, other the tumor microenvironment (TME), such as myeloid cells, possess functional antiviral pathways. In this study, we aimed to characterize interplay between MV and human MPM. We cocultured MPM cell lines with monocytes macrophages infected them MV. analyzed transcriptome of each type studied their secretion...
Human serum contains large amounts of anti-carbohydrate antibodies, some which may recognize epitopes on viral glycans. Here, we tested the hypothesis that such antibodies confer protection against COVID-19 so patients would be preferentially found among people with low specific since individual repertoires vary considerably. After selecting glycan commonly represented in human antibody repertoire also expressed glycans, plasma levels corresponding were determined by ELISA 88 SARS-CoV-2...
Although CD4+CD8+ double positive (DP) T cells represent a small fraction of peripheral lymphocytes in healthy human donors, their frequency is often increased under pathological conditions (in blood and targeted tissues). In solid cancers such as melanoma, we previously demonstrated an enrichment tumor reactive CD4lowCD8highαβ DP among tumor-infiltrating unknown function. Similarly to single (SP) CD8+ counterparts, intra-melanoma recognized melanoma cell lines HLA-class-I restricted...
Abstract Adoptive cell transfer (ACT) of tumor-specific T lymphocytes represents a relevant therapeutic strategy to treat metastatic melanoma patients. Ideal T-cells should combine tumor specificity and reactivity with survival in vivo, while avoiding autoimmune side effects. Here we report results from Phase I/II clinical trial (NCT02424916, performed between 2015 2018) which 6 HLA-A2 patients received autologous antigen-specific produced PBMC, after peptide stimulation vitro, followed by...
While immune checkpoint (IC) therapies, particularly those targeting the PD-1/PD-L1 axis, have revolutionized treatment of melanoma and several other cancers, their effect remains very limited in colorectal cancer (CRC). To define a comprehensive landscape ICs human CRC tumor microenvironment (TME), we evaluated, using multiparametric flow cytometry, ex vivo expression via tumor-infiltrating lymphocytes (TILs) (n = 40 CRCs) as well that respective ligands on myeloid cells 29). Supervised...
Abstract Over the past two decades, there has been a great interest in study of HLA-E-restricted αβ T cells during bacterial and viral infections, including recently SARS-CoV-2 infection. Phenotyping these specific requires new tools such as tetramers for rapid cell staining or sorting, well identification peptides capable to bind HLA-E pocket. To this aim, we have developed an optimal photosensitive peptide generate stable HLA-E/pUV complexes allowing high-throughput production...
In mice, microbiota-induced Tregs both maintain intestinal homeostasis and provide resistance to immuno-pathologies in the adult. Identifying their human functional counterpart therefore represents an important goal. We discovered, colonic lamina propria blood, a FoxP3-negative IL-10-secreting Treg subset, which co-expresses CD4 CD8α (hence named DP8α) displays TCR-reactivity against Faecalibacterium prausnitzii , indicating role for this symbiotic bacterium induction. Moreover, supporting...
Abstract Immunotherapies targeting the PD-1 pathway have profoundly transformed clinical care of cancer patients for a growing variety types. However, most do not experience durable benefit. The definition robust and convenient biomarkers therapy efficacy to stratify beforehand or early after initiation that could guide therapeutic management is still lacking while being very active research field. Biomarkers described date include tumor burden, neoantigen load, presence number PD-1+ CD8+ at...
Abstract In colorectal cancer (CRC), little is known about mechanisms by which tumor cells can influence the biology of Tumor Infiltrating T lymphocytes (TILs) present in microenvironment. One these could be modulation inflammasome cells. The a molecular platform normal intestinal epithelial cells, whose effector protein, caspase-1, rapidly mature IL-18 and generate mucosal Th1 (IFNγ) response. However, status CRC its potential role TILs are unknown yet. This prospective study aimed to...
Antibody-dependent cellular cytotoxicity (ADCC) in the anti-tumor effect of cetuximab metastatic colorectal cancer (mCRC) is only based on impact FcγRIIIA (CD16) polymorphisms as predictive therapeutic response. However, nature, density and + effector cells tumor remain poorly documented. Moreover, inhibition cetuximab-mediated ADCC induced by NK engagement new inhibitory CD94-NKG2A immune checkpoint has been demonstrated vitro . This multicentric study aimed to determine, paired primary...
<div>Abstract<p>MicroRNAs (miRNA), small noncoding RNAs that regulate gene expression, exist not only in cells but also a variety of body fluids. These circulating miRNAs could enable intercellular communication. are packaged membrane-encapsulated vesicles, such as exosomes, or protected by RNA-binding proteins. Here, we report included human melanoma exosomes the tumor immune response. Using microscopy and flow cytometry, demonstrate CD8<sup>+</sup> T internalize...
<div>Abstract<p>MicroRNAs (miRNA), small noncoding RNAs that regulate gene expression, exist not only in cells but also a variety of body fluids. These circulating miRNAs could enable intercellular communication. are packaged membrane-encapsulated vesicles, such as exosomes, or protected by RNA-binding proteins. Here, we report included human melanoma exosomes the tumor immune response. Using microscopy and flow cytometry, demonstrate CD8<sup>+</sup> T internalize...
<b>Introduction:</b> Molecular mechanisms underlying inflammation processes during severe asthma remains poorly understood. We previously demonstrated in a murine model that inhibiting the small GTPase Rac lowered infiltration of immune cells within bronchi (Dilasser, F. et al. Thorax 2021;76:326-334). <b>Aims and objectives:</b> aim to identify inflammatory displaying activation highlight affected pathways. <b>Methods:</b> quantified active Rac-GTP by fluorescence bronchial biopsies from...
640 Background: In colorectal cancer (CRC), little is known about mechanisms by which tumor cells can influence the phenotype and biology of Tumor Infiltrating T lymphocytes (TILs) microenvironment. One these could be inflammasome, a molecular platform present in normal intestinal epithelial cells, whose effector protein, caspase-1, rapidly mature IL18 generate mucosal Th1/Tc1 (IFNg) response. However, inflammasome status CRC its potential role on TILs are unknown yet. Methods: Prospective...
e14592 Background: A better understanding of the immune-modulating interactions between tumor cells and immune underlying balance control resistance in colorectal cancer (CRC) is crucial for design immunotherapies. We have previously demonstrated that overexpression human MHC class Ib molecule - HLA-E/β2 microglobulin by CRC was associated with an unfavorable prognosis, suggesting its involvement escape. However, specific receptor HLA-E/β2m CD94/NKG2A, inhibitory or CD94/NKG2C, activating...
e23087 Background: In colorectal cancer (CRC), little is known about mechanisms by which tumor cells can influence the phenotype and biology of Tumor Infiltrating T lymphocytes (TILs) microenvironment. One these could be inflammasome, a molecular platform present in normal intestinal epithelial cells, whose effector protein, caspase-1, rapidly mature IL18 generate mucosal Th1/Tc1 (IFNγ) response. However, inflammasome status CRC its potential role on TILs are unknown yet. Methods:...