A5114832867- Skin Protection and Aging
- Acne and Rosacea Treatments and Effects
- Dermatology and Skin Diseases
- Cellular Mechanics and Interactions
- melanin and skin pigmentation
- Photodynamic Therapy Research Studies
- Indoor Air Quality and Microbial Exposure
- Bee Products Chemical Analysis
- Advancements in Transdermal Drug Delivery
- 3D Printing in Biomedical Research
- Dermatologic Treatments and Research
- Optical Imaging and Spectroscopy Techniques
- Effects and risks of endocrine disrupting chemicals
- Cutaneous Melanoma Detection and Management
- Infrared Thermography in Medicine
- Skin and Cellular Biology Research
- Olfactory and Sensory Function Studies
- Animal testing and alternatives
- Cell Image Analysis Techniques
- Hair Growth and Disorders
- Pesticide Exposure and Toxicity
- Micro and Nano Robotics
- Nonmelanoma Skin Cancer Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Textile materials and evaluations
Pierre Fabre (France)
2014-2024
Skin Research Center
2017-2021
Université de Toulouse
2021
Pierre Fabre (United Kingdom)
2017
L'Oréal (France)
2016
Pierre Fabre (Germany)
2015
Université Paris Cité
2009-2012
Laboratoire Matière et Systèmes Complexes
2011-2012
Délégation Paris 7
2010-2011
Centre National de la Recherche Scientifique
2009-2011
Cell migration plays a major role in many fundamental biological processes, such as morphogenesis, tumor metastasis, and wound healing. As they anchor pull on their surroundings, adhering cells actively probe the stiffness of environment. Current understanding is that traction forces exerted by arise mainly at mechanotransduction sites, called focal adhesions, whose size seems to be correlated force underlying substrate, least during initial stages. In fact, our data show direct measurements...
In a three-dimensional environment, cells migrate through complex topographical features. Using microstructured substrates, we investigate the role of substrate topography in cell adhesion and migration. To do so, fibroblasts are plated on chemically identical substrates composed microfabricated pillars. When dimensions pillars (i.e., diameter, length, spacing) varied, migrating encounter alternating flat rough surfaces that depend spacing between Consequently, show affects shape migration...
Abstract In recent years, interest is growing in the biological cutaneous effects of high‐energy visible light (400–450 nm). present study, we explored impact blue (BL) on repair pyrimidine dimers, major class premutagenic DNA damage induced by exposure to sunlight. We unambiguously demonstrate that vitro reconstructed human epidermis environmentally relevant doses BL strongly decreases rate cyclobutane dimers and (6‐4) pyrimidone photoproducts a subsequent UVB irradiation. Using highly...
Magnetic actuated microdevices can be used to achieve several complex functions in microfluidics and microfabricated devices. For example, magnetic mixers actuators have been proposed help handling fluids at a small scale. Here, we present strategy create magnetically micropillar arrays. We combined microfabrication techniques the dispersion of aggregates embedded inside polymeric matrices design micrometre scale features. By creating field gradient vicinity substrate, well-defined forces...
Deimination (or citrullination) is a recently described post-translational modification, but its consequences are not yet well understood. It catalysed by peptidylarginine deiminases (PADs). These enzymes transform arginyl residues involved in peptidyl link into citrullyl calcium-dependent manner. Several PAD substrates have already been identified like filaggrin and keratins K1 K10 the epidermis, trichohyalin hair follicles, also ubiquitous proteins histones. PADs act large panel of...
Abstract Skin metabolism is important to consider when assessing local toxicity and/or penetration of chemicals and their metabolites. If human skin supply limited, pig can be used as an alternative. To identify any species differences, we have investigated the 10 in a explant model. Phase I metabolic pathways from both included those known occur via cytochrome P450s, esterases, alcohol dehydrogenases aldehyde dehydrogenases. Common II were glucuronidation sulfation but other conjugation...
Although acne vulgaris has a multifactorial aetiology, comedogenesis and bacteria colonization of the pilosebaceous unit are known to play major role in onset inflammatory lesions. However, many aspects remain poorly understood such as where when is early stage Propionibacterium acnes follicular unit? Our research aimed at providing precise analysis microcomedone's structure better understand interplay between units, therefore, its formation
Transcription control at a distance is critical mechanism, particularly for contiguous genes. The peptidylarginine deiminases (PADs) catalyse the conversion of protein-bound arginine into citrulline (deimination), reaction in pathophysiology multiple sclerosis, Alzheimer's disease and rheumatoid arthritis, metabolism major epidermal barrier protein filaggrin, strong predisposing factor atopic dermatitis. PADs are encoded by 5 clustered PADI genes (1p35-6). Unclear mechanisms controlling...
Deleterious effects of pollutants and ultraviolet radiation on the skin can be attenuated using formulations containing antioxidants. However, these have disadvantages, including chemical instability, photodegradation, poor bioavailability or biological activity. Here, two commercial were evaluated: one optimized to stabilize deliver ascorbic acid (AA) at 15% other a glucoside form AA, namely 2-glucoside (AA2G), 1.8% physiological pH. We compared delivery, antioxidative stability AA2G with...
Abstract Background Overexposure to sunlight can have many harmful biological effects on the skin, leading skin cancer and photoaging. As ultraviolet (UV) radiation has been identified as a cause of DNA damage oxidative stress in photoprotection provided by sunscreens is evaluated through their ability filter UV light, using sun protection factor (SPF). However, recent data shown that high‐energy visible (HEV) light also damage. Objectives To develop new vivo method for evaluating across...
Acne is a multifactorial chronic inflammatory disease of the pilosebaceous unit, where Cutibacterium acnes plays main role. Recent papers demonstrated that specific C. phylotypes were correlated with severity acne and reported loss phylotype diversity in this context.The aim exploratory study was to evaluate efficacy new dermocosmetic product containing Myrtus communis Celastrol-enriched plant cell culture extracts on abundance clinical parameters subjects mild moderate...
Abstract OECD test guideline compliant skin penetration studies, which also comply with the SCCS basic criteria, are lacking for genistein and daidzein. Therefore, we have measured their metabolism using ex vivo explants of fresh (i.e., metabolically viable) pig skin, frozen human Phenion full‐thickness (FT) models. Preliminary studies helped to define optimal experimental conditions. The dermal absorption 10 nmoles/cm 2 daidzein in ethanol was comparable all four A first‐pass glucuronide...
The use of multiphoton imaging has become a standard technique to visualize the dermis fibers as it requires no specific staining. density and organization collagen elastin are common markers skin intrinsic aging photoaging; thus, there is need grading this with quantitative indicators able provide robust evaluation fibers' state. We propose systematic analysis images biopsies taken on buttock forearm patients different ages. intensity histograms were analyzed through their moments, wavelet...
Abstract The abundance of xenobiotic metabolizing enzymes (XMEs) is different in the skin and liver; therefore, it important to differentiate between liver metabolism when applying information safety assessment topically applied ingredients cosmetics. Here, we have employed EpiSkin™ S9 human investigate organ‐specific metabolic stability 47 cosmetic‐relevant chemicals. rank order rate six chemicals primary hepatocytes matched relatively well. XME pathways were also present EpiSkin S9;...
Parabens are alkyl esters of 4-hydroxybenzoic acid (4-HBA), with short-chain parabens used as antimicrobials in cosmetics. We investigated the impact chain structure on skin and liver metabolism. Incubations primary human hepatocytes S9 indicated that methyl-, ethyl-, propyl- butylparaben were rapidly metabolized to similar metabolites, including 4-HBA plus corresponding alcohols. Liver EpiSkin™ investigate metabolism 16 short long straight- branched-chain parabens. The rate hydrolysis...
Abstract In a read‐across assessment of the safety genistein and daidzein in cosmetic products, additional information was required to account for differences their systemic exposure after topical application typical body lotion formulation. Therefore, we measured penetration metabolism two doses (3 30 nmoles/cm 2 ) applied ethanol formulation fresh pig skin, frozen human PhenionFT models. Both chemicals readily penetrated all skin models when ethanol. The same sulfate glucuronide...