WNT5A is known to be involved in the pathogenesis of osteoarthritis. This study investigated molecular mechanism exosomal miR-92a-3p and chondrogenesis cartilage degeneration.Exosomal expression was assessed vitro a human mesenchymal stem cell (MSC) model normal OA primary chondrocytes (PHCs). MSCs PHCs were treated with exosomes derived from MSC-miR-92a-3p (MSC-miR-92a-3p-Exos) or its antisense inhibitor (MSC-anti-miR-92a-3p-Exos), respectively. Small interfering RNAs (siRNAs) luciferase...

The heterogeneity of meniscus cells and the mechanism degeneration is not well understood. Here, single-cell RNA sequencing (scRNA-seq) was used to identify various cell subsets investigate degeneration.scRNA-seq their gene signatures in healthy human degenerated determine differentiation relationships characterise diversity within specific types. Colony-forming, multi-differentiation assays a mice injury model were progenitor cells. We investigated role (DegP) clusters during using...

Abstract Micro RNA s play critical roles in the pathogenesis of osteoarthritis, most common chronic degenerative joint disease. Exosomes derived from miR‐95‐5p‐overexpressing primary chondrocytes ( AC ‐miR‐95‐5p) may be effective treating osteoarthritis. Increased expression HDAC 2/8 occurs tissues and chondrocyte‐secreted exosomes patients with osteoarthritis mediates cartilage‐specific gene chondrocytes. We have been suggested that ‐miR‐95‐5p ‐miR‐95‐5p‐Exos) would enhance chondrogenesis...

Abstract Objectives Aberrations in exosomal circular RNA (circRNA) expression have been identified various human diseases. In this study, we investigated whether circRNAs could act as competing endogenous RNAs (ceRNAs) to regulate the pathological process of osteoarthritis (OA). This study aimed elucidate specific MSC-derived responsible for MSC-mediated chondrogenic differentiation using bone marrow-derived MSCs (hMSCs) and a destabilization medial meniscus (DMM) mouse model OA. Methods...

Abstract MicroRNAs (miRNAs, miR) play a key role in the pathogenesis of osteoarthritis (OA). Few studies have examined regulatory P21-activated kinases (PAKs), family serine/threonine kinases, OA. The aim this study was to determine whether miR-455-3p can regulate cartilage degeneration OA by targeting PAK2. MiR-455-3p knockout mice showed significant knee cartilage. expression increased and PAK2 decreased late stage human adipose-derived stem cell (hADSC) chondrogenesis chondrocytes...

Aggrecanase-1 (ADAMTS-4) and aggrecanase-2 (ADAMTS-5) are secreted enzymes belonging to the ADAMTS (a disintegrin metalloproteinase with thrombospondin motifs) family that play significant roles in progression of osteoarthritis (OA). Here, we aimed determine whether expression ADAMTS-4/5 chondrogenesis inflammation is regulated by microRNA-92a-3p (miR-92a-3p).MiR-92a-3p expressions were determined quantitative polymerase chain reaction (qPCR). To investigate repressive effect miR-92a-3p on...

Long non-coding RNAs (lncRNAs) play pivotal roles in diseases such as osteoarthritis (OA). However, knowledge of the biological lncRNAs is limited OA. We aimed to explore function and molecular mechanism HOTTIP chondrogenesis cartilage degradation. used human mesenchymal stem cell (hMSC) model chondrogenesis, parallel with, tissue biopsies from normal OA detect HOTTIP, CCL3, miR-455-3p expression vitro. Biological interactions between were determined by RNA silencing overexpression evaluated...

Long non-coding RNAs (lncRNAs) play pivotal roles in mesenchymal stem cell differentiation. However, the mechanisms by which RNA (ncRNA) networks regulate osteogenic differentiation remain unclear. Therefore, our aim was to identify RNA-associated gene and transcript expression profiles during osteogenesis bone marrow cells (BMSCs). Using transcriptome sequencing for differentially expressed ncRNAs mRNAs between days 0 21 of BMSCs, we found that microRNA (miRNA) miR-503-5p significantly...

Abstract Impaired autophagy is reported to promote osteoarthritis (OA). However, the mechanism by which in regulating meniscus degeneration and OA remains unclear. Here, unconvered aberrant energetic metabolism pattern cells with uncovered first, results lower adenosine triphosphate (ATP) production. And these phenomena are induced impaired OA. It further revealed that suppression of m 6 A demethylase fat mass obesity‐associated protein (FTO) inhibits causing ATP production reducing...

Chondrocyte senescence play an essential role in osteoarthritis (OA) progression. Recent studies have shown that snoRNA-derived RNA fragments (sdRNAs) are novel regulators of post-transcriptional gene expression. However, the expression profiles and their regulation chondrocyte OA progression is unknown. Here, we determined sdRNAs profile explored sdRNA-D43 its mechanism. We used qPCR arrays to determine chondrocytes areas undamaged damaged three knee samples. SdRNA-D43 was using...

In growing bones of mice, multiple cell types contribute to the osteoblast lineage, including growth plate chondrocytes, perichondrial cells and CXCL12-abundant reticular (CAR) marrow stromal cells. Here we use single-cell RNA sequencing lineage tracing show that all these precursors, even postnatally, derive from Sox9-expressing progenitors. We also characterize a distinct group chondrocytes located between perichondrium columns lineage.

Abstract Micro(mi)RNAs are small, non‐coding RNA molecules known to play a significant role in osteoarthritis (OA) initiation and development, similar matrix metalloproteinases (MMPs), they participate cartilage degeneration cleave multiple extracellular matrices. The aim of this study was determine whether the expression MMP‐19 interleukin (IL)‐1β‐induced human chondrocytes is directly regulated by miR‐193b‐3p. Expression levels miR‐193b‐3p normal cartilage, interleukin‐1 β (IL‐1β)‐induced...

There is increasing evidence regarding the pivotal roles of microRNAs (miRNAs) and histone deacetylases (HDACs) in development osteoarthritis (OA). This study aimed to determine whether miR-193b-5p regulates HDAC7 expression directly affect cartilage degeneration. Expression levels miR-193b-5p, HDAC7, matrix metalloproteinase 3 (MMP3), MMP13 were determined normal OA primary human chondrocytes (PHCs) stimulated with interleukin-1β (IL-1β). PHCs transfected a mimic or inhibitor verify...

Mitochondrial dysfunction is related to the pathogenesis of osteoarthritis (OA); however, there are no effective drugs treat OA for maintaining mitochondrial homeostasis. Studies have shown that mitochonic acid-5 (MA-5) has a protective effect against damage and plays role in mitophagy. However, it not clear whether MA-5 beneficial on inflammatory articular cartilage. Here, human cartilage was obtained from patients undergoing total joint replacement. Interleukin-1β (IL-1β) used stimulate...

Abstract Knee osteoarthritis (KOA) is a common joint disorder characterized by progressive cartilage degeneration. MiR‐455‐3p has been identified as promising therapeutic candidate to maintain homeostasis. However, clinical application of free miR‐455‐3p limited rapid degradation, poor targeting efficiency, and off‐target effects. To overcome these limitations, an injectable nanocomposite hydrogel‐based miRNA delivery system consisting affinity nanocarrier (CANC) encapsulated within...

MicroRNAs (miRNAs) play a pivotal role in cartilage development and homeostasis osteoarthritis (OA). While the fundamental roles of miRNAs degeneration have been extensively studied, their effects on chondrogenic differentiation induced by human adipose-derived stem cells (hADSCs) underlying mechanisms remain largely elusive. Here, we investigated hADSC chondrocyte homeostasis. Using microarray analysis, screened expressed differentiated hADSCs identified miR-490-5p as most significantly...

Osteoarthritis (OA) is a degenerative disease causing the progressive destruction of articular cartilage; however, aetiology has not yet been elucidated. Circular RNAs (circRNAs) are reportedly involved in cartilage degeneration and OA development. In present study, we identified that circNFIX regulates chondrogenesis homeostasis.Microarray analysis was performed to explore circRNA expression during chondrogenic differentiation human adipose-drived stem cells (hADSCs). CircNFIX determined...

Abstract Osteoarthritis (OA) is an age-related cartilage-degenerating joint disease. Mitochondrial dysfunction has been reported to promote the development of OA. Poly (ADP-ribose) polymerase family member 12 (PARP12) a key regulator mitochondrial function, protein translation, and inflammation. However, role PARP12 in OA-based cartilage degradation underlying mechanisms are relatively unknown. Here, we first demonstrated that inhibits mitophagy promotes OA progression human monosodium...

tRNA-derived fragments (tRFs) are new noncoding RNAs, and recent studies have shown that tRNAs tRFs important functions in cell metabolism via posttranscriptional regulation of gene expression. However, whether regulate cellular the anterior cruciate ligament (ACL) remains elusive. The aim this study was to investigate role action mechanism ACL metabolism. A tRF array used determine expression profiles different human cells, quantitative real-time polymerase chain reaction fluorescence situ...