A5100687826- Osteoarthritis Treatment and Mechanisms
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Aquaculture Nutrition and Growth
- Extracellular vesicles in disease
- Reproductive biology and impacts on aquatic species
- Fish Biology and Ecology Studies
- Circular RNAs in diseases
- Neurobiology and Insect Physiology Research
- Inflammatory mediators and NSAID effects
- RNA modifications and cancer
- Total Knee Arthroplasty Outcomes
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Fish Ecology and Management Studies
- interferon and immune responses
- Developmental Biology and Gene Regulation
- Orthopedic Infections and Treatments
- Diet and metabolism studies
- Bone Metabolism and Diseases
- Aquaculture disease management and microbiota
- Orthopaedic implants and arthroplasty
- Animal Genetics and Reproduction
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bone and Joint Diseases
- Chromosomal and Genetic Variations
Third Affiliated Hospital of Southern Medical University
2020-2025
Key Laboratory of Guangdong Province
2023-2025
Irvine Valley College
2024
Guangdong Province Stomatological Hospital
2023
Guangzhou University
2023
VA Palo Alto Health Care System
2022
Stanford University
2022
Georgia State University
2022
Henan University of Science and Technology
2014-2021
Southern Medical University
2021
WNT5A is known to be involved in the pathogenesis of osteoarthritis. This study investigated molecular mechanism exosomal miR-92a-3p and chondrogenesis cartilage degeneration.Exosomal expression was assessed vitro a human mesenchymal stem cell (MSC) model normal OA primary chondrocytes (PHCs). MSCs PHCs were treated with exosomes derived from MSC-miR-92a-3p (MSC-miR-92a-3p-Exos) or its antisense inhibitor (MSC-anti-miR-92a-3p-Exos), respectively. Small interfering RNAs (siRNAs) luciferase...
Histone deacetylase 3 (HDAC3) plays a pivotal role in the repression of cartilage-specific gene expression human chondrocytes. The aim this study was to determine whether microRNA-193b-3p (miR-193b-3p) regulates HDAC3 during chondrogenesis and chondrocyte metabolism. Methods: miR-193b-3p assessed mesenchymal stem cell (hMSC) model chondrogenesis, interleukin-1β (IL-1β)-treated primary chondrocytes (PHCs), non-degraded degraded cartilage. hMSCs PHCs were transfected with or its antisense...
Abstract The aim of the current study was to compare expression microRNAs (miRNAs) in exosomes derived from human bone mesenchymal stem cells (hBMSCs) with and without chondrogenic induction. Exosomes hBMSCs were isolated identified. Microarray analysis performed miRNA between induction, quantitative real‐time polymerase chain reaction (qRT‐PCR) used verify differentially expressed miRNAs. transfected mimic extract miRNA‐overexpressed exosomes. results showed that most exhibited a cup‐shaped...
Abstract Micro RNA s play critical roles in the pathogenesis of osteoarthritis, most common chronic degenerative joint disease. Exosomes derived from miR‐95‐5p‐overexpressing primary chondrocytes ( AC ‐miR‐95‐5p) may be effective treating osteoarthritis. Increased expression HDAC 2/8 occurs tissues and chondrocyte‐secreted exosomes patients with osteoarthritis mediates cartilage‐specific gene chondrocytes. We have been suggested that ‐miR‐95‐5p ‐miR‐95‐5p‐Exos) would enhance chondrogenesis...
Abstract MicroRNAs (miRNAs, miR) play a key role in the pathogenesis of osteoarthritis (OA). Few studies have examined regulatory P21-activated kinases (PAKs), family serine/threonine kinases, OA. The aim this study was to determine whether miR-455-3p can regulate cartilage degeneration OA by targeting PAK2. MiR-455-3p knockout mice showed significant knee cartilage. expression increased and PAK2 decreased late stage human adipose-derived stem cell (hADSC) chondrogenesis chondrocytes...
Increasing evidence suggest the biological roles of N6-methyladenosine (m6A) and long noncoding RNAs (lncRNAs) in bone disease, especially osteoarthritis (OA). However, interaction m6A lncRNA is still unclear. Here, we found that a m6A-related LINC00680 upregulated OA tissue IL-1β-induced isolated primary chondrocytes. Functionally, chondrocytes, silencing recovered proliferation repressed extracellular matrix (ECM) degradation. Mechanistically, methyltransferase METTL3 combined tithe site...
Long non-coding RNAs (lncRNAs) play pivotal roles in diseases such as osteoarthritis (OA). However, knowledge of the biological lncRNAs is limited OA. We aimed to explore function and molecular mechanism HOTTIP chondrogenesis cartilage degradation. used human mesenchymal stem cell (hMSC) model chondrogenesis, parallel with, tissue biopsies from normal OA detect HOTTIP, CCL3, miR-455-3p expression vitro. Biological interactions between were determined by RNA silencing overexpression evaluated...
The neurogenin (Ngn) transcription factors control early neurogenesis and neurite outgrowth in mammalian cortex. In contrast to their proneural activity, function growth is poorly understood. Drosophila has a single predicted Ngn homolog, Tap, of unknown function. Here we show that Tap not protein but required for proper axonal guidance neurons the mushroom body, neuropile associative learning memory. Genetic expression analyses suggest inhibits excessive by fine regulation levels Wnt...
Chondrocyte senescence play an essential role in osteoarthritis (OA) progression. Recent studies have shown that snoRNA-derived RNA fragments (sdRNAs) are novel regulators of post-transcriptional gene expression. However, the expression profiles and their regulation chondrocyte OA progression is unknown. Here, we determined sdRNAs profile explored sdRNA-D43 its mechanism. We used qPCR arrays to determine chondrocytes areas undamaged damaged three knee samples. SdRNA-D43 was using...
This study explores the role of circPAFAH1B2 in osteoarthritis (OA) by investigating its influence on nuclear-mitochondrial communication, a largely unexplored area OA progression. By uncovering how regulates mitochondrial function, aims to identify novel therapeutic targets for prevention and treatment. aimed regulatory communication within chondrocytes cartilage homeostasis. expression was determined via quantitative real-time polymerase chain reaction (qRT-PCR) situ hybridization. RNA...
Abstract The effect of residual varus on survival rate and function in patients with knee osteoarthritis (OA) was considered an important issue for successful primary total arthroplasty (TKA). In this study, we compared the midterm clinical functional outcomes different varus. A retrospective review 175 (219 knees) OA > 3° hip-knee-ankle (HKA) who underwent TKA after exclusions loss to follow-up from 237 (281 knees). mean period 5.2 ( ± 1.1) years. Patients were divided into four groups...
Knee osteoarthritis (KOA) is a highly prevalent disabling joint disease in aged people. Progressive cartilage degradation the hallmark of KOA, but its deeper mechanism remains unclear. Substantial evidence indicates importance synovium for homeostasis. The present study aimed to determine whether regulates metabolism through chondrogenesis-related microRNAs (miRNAs) KOA microenvironment. Clinical sample testing and vitro cell experiments screened out miR-455 miR-210 as effective miRNAs....
Chronic venous insufficiency (CVI) is the consequence of valve reflux and/or flow obstruction and resulting hypertension in lower extremities. The aim this prospective supplement registry study was to evaluate efficacy compression stockings or Pycnogenol
Synovitis, among the most common signs of early-stage osteoarthritis (OA), is mainly mediated by fibroblast-like synoviocytes (FLSs). Cartilage destruction creates chondrocyte lysates (CLs) that activate synovial inflammation. A comprehensive understanding chondrocyte-FLS communication might offer novel, specific therapeutic targets for treating synovitis and OA. Hence, we sought to uncover role CLs in OA-FLSs synovitis. Isolated were cocultured with FLSs test whether they could stimulate...
MicroRNAs (miRNAs) play a pivotal role in cartilage development and homeostasis osteoarthritis (OA). While the fundamental roles of miRNAs degeneration have been extensively studied, their effects on chondrogenic differentiation induced by human adipose-derived stem cells (hADSCs) underlying mechanisms remain largely elusive. Here, we investigated hADSC chondrocyte homeostasis. Using microarray analysis, screened expressed differentiated hADSCs identified miR-490-5p as most significantly...
Meniscus plays an important role in joint homeostasis. Tear or degeneration of meniscus might facilitate the process knee osteoarthritis (OA). Hence, to investigate transcriptome change during degeneration, we reveal alterations messenger RNA (mRNA), microRNA (miRNA), long noncoding (lncRNA), and circular (circRNA) OA by whole-transcriptome sequence. A total 375 mRNAs, 15 miRNAs, 56 lncRNAs, 90 circRNAs were significantly altered degenerative treated with interleukin-1β (IL-1β). More...
Genetic screens are powerful methods for the discovery of gene-phenotype associations. However, a systems biology approach to genetics must leverage massive amount "omics" data enhance power and speed functional gene in vivo. Thus far, few computational function prediction have been rigorously tested their performance on genome-wide scale In this work, we demonstrate that integrating prioritization with large-scale genetic screening is tool discovery. To discover genes involved neural...
Osteoarthritis (OA) is a degenerative disease causing the progressive destruction of articular cartilage; however, aetiology has not yet been elucidated. Circular RNAs (circRNAs) are reportedly involved in cartilage degeneration and OA development. In present study, we identified that circNFIX regulates chondrogenesis homeostasis.Microarray analysis was performed to explore circRNA expression during chondrogenic differentiation human adipose-drived stem cells (hADSCs). CircNFIX determined...
Abstract The Zig-zag eel ( Mastacembelus armatus ) is an economically important species in southern China. Its natural resources have declined year by due to overfishing. Understanding its genetic diversity and population structure very for resource conservations. Here, we first successfully developed 28 polymorphic microsatellite markers zig-zag eels ten of them were used examine the differentiation 7 populations collected from major river systems south In total, 224 alleles found with 10...
Osteoarthritis (OA), which involves total joint damage and dysfunction, is a leading cause of disability worldwide. However, its exact pathogenesis remains unclear. Here, we identified TCF12 as an important regulator the progression OA. qRT-PCR, immunoblotting immunohistochemistry (IHC) were used to detect expression level TCF12. The interaction with downstream factor CXCR4 was assessed by Western blotting, immunofluorescence, qRT-PCR luciferase assays. A mouse model generated examine...