A5101873668- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Foot and Ankle Surgery
- Metabolism, Diabetes, and Cancer
- Orthopedic Surgery and Rehabilitation
- Curcumin's Biomedical Applications
- Cancer, Hypoxia, and Metabolism
- PI3K/AKT/mTOR signaling in cancer
- Fibroblast Growth Factor Research
- RNA Interference and Gene Delivery
- Metabolism and Genetic Disorders
- Shoulder Injury and Treatment
- Cancer Mechanisms and Therapy
- Nanoplatforms for cancer theranostics
- Plant-based Medicinal Research
- Bacteriophages and microbial interactions
- Biomarkers in Disease Mechanisms
- RNA Research and Splicing
- Kruppel-like factors research
- Inflammasome and immune disorders
- Lung Cancer Research Studies
Zhejiang Chinese Medical University
2022-2025
Binzhou University
2017-2025
Binzhou Medical University
2017-2025
Chongqing University
2025
Hubei University of Medicine
2021-2025
First Affiliated Hospital of Zhengzhou University
2016-2025
Zhoushan Hospital
2025
Huzhou Central Hospital
2024
Zhengzhou Central Hospital
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2004-2024
Microglia are innate immune cells in the central nervous system (CNS), that supplies neurons with key factors for executing autophagosomal/lysosomal functions. Macroautophagy/autophagy is a cellular catabolic process maintains cell balance response to stress-related stimulation. Abnormal autophagy occurs many pathologies, such as cancer, and autoimmune neurodegenerative diseases. Hence, clarification of mechanisms regulation utmost importance. Recently, researchers presented microRNAs...
We and others have previously shown that the STAT3 signaling pathway is activated in some esophageal squamous cell carcinoma (ESCC) cells required for survival growth of these primary ESCC-derived xenografts. It has also been natural polyphenol curcumin an effective anti-tumor agent.Luciferase assay immunoblotting were performed to examine whether suppressed signaling. CCK-8 xenografts utilized analyzing ESCC culture mice. Soft agar was carried out determine colony formation ability presence...
To identify bioactive equivalent combinatorial components (BECCs) in herbal medicines. The exact composition of effective medicines is often elusive due to the lack adequate screening methodology. Herein, we propose a hypothesis that BECCs accounting for whole efficacy original could be discovered from complex mixture constituents.We developed equivalence oriented feedback method and applied it discover an preparation Cardiotonic Pill (CP). operations include chemical profiling CP, followed...
Age-related impairment of macroautophagy/autophagy and loss cardiac tissue homeostasis contribute significantly to cardiovascular diseases later in life. MTOR (mechanistic target rapamycin kinase) signaling is the most well-known regulator autophagy, cellular homeostasis, longevity. The consists two structurally functionally distinct multiprotein complexes, MTORC1 MTORC2. While well characterized but role MTORC2 aging autophagy remains poorly understood. Here we identified TGFB-INHB/activin...
BACKGROUND:microRNA (miR)-181a has been reported to be downregulated in Parkinson's disease (PD), but the regulatory mechanism of miR-181a on neuron apoptosis and autophagy is still poorly understood. We aimed investigate neuroprotective effects PD vitro. MATERIAL AND METHODS:Human SK-N-SH neuroblastoma cells were incubated with different concentrations 1-methyl-4-phenylpyridinium ion (MPP+) induce model. The expression was then analyzed. After transfection mimic or scramble following MPP+...
Silicosis is a leading cause of occupational disease-related morbidity and mortality worldwide, but the molecular basis underlying its development remains unclear. An accumulating body evidence supports gasdermin D (GSDMD)-mediated pyroptosis as key component in various pulmonary diseases. However, there little experimental connecting silicosis GSDMD-driven pyroptosis. In this work, we investigated role GSDMD-mediated silicosis. Single-cell RNA sequencing healthy human murine lung tissues...
Long non-coding RNAs (lncRNAs) are aberrantly expressed and have important functions in pathological processes. The present study investigated the lncRNA profiles effects of curcumin (Cur) on radiosensitivity nasopharyngeal carcinoma (NPC) cells. mRNA each cell group were described by microarray analysis. Numerous differentially genes observed microarrays three groups. Cur significantly reversed IR-induced expression signatures, shown clustering Moreover, 116 these Cur-reversed lncRNAs...
G protein-coupled receptor (GPR) 30 is a novel estrogen receptor. Recent studies suggest that activation of the GPR30 confers rapid cardioprotection in isolated rat heart. It unknown whether chronic beneficial or not for heart failure. In this study we investigated cardiac effect sustained inhibition GPR30. Female Sprague–Dawley rats were divided into 7 groups #2Q1: sham surgery (Sham), bilateral ovariectomy (OVX), OVX+estrogen (E2), OVX+isoproterenol (ISO), OVX+ISO+G-1, OVX+ISO+E2+G15,...
Ischemia/reperfusion (I/R) is a pathological process that causes severe damage. Propofol known to alleviate I/R-related injury; however, the exact function and underlying mechanisms are not fully understood.Using an oxygen glucose deprivation/re-oxygenation (OGD/R) method, in vitro I/R injury model was induced. The cell viability level of Fe2+, glutathione synthetase (GSH), malondialdehyde (MDA) were evaluated using kits. Luciferase reporter gene assay, chromatin immunoprecipitation, RNA...
CSCs (Cancer stem cells) with distinct metabolic features are considered to cause HCC (hepatocellular carcinoma) initiation, metastasis and therapeutic resistance. Here, we perform a gene CRISPR/Cas9 knockout library screen in tumorspheres derived from cells find that deletion of SCARB2 suppresses the cancer cell-like properties cells. Knockout Scarb2 hepatocytes attenuates initiation progression both MYC-driven DEN (diethylnitrosamine)-induced mouse models. Mechanistically, binding MYC...
MicroRNAs are known to be involved in carcinogenesis and tumor progression glioma. Recently, microRNA-372 (miR-372) has been proved play a substantial role several human cancers, but its functions glioma remain unclear. In this study, we confirmed that miR-372 was commonly upregulated cell lines tissues. Downregulation of markedly inhibited proliferation invasion induced G1/S arrest apoptosis. Consistently, the xenograft mouse model also unveiled suppressive effects knockdown on growth....
Chemical-induced kidney injury represents a substantial health risk, with ferroptosis, type of cell death caused by lipid peroxidation, playing role in numerous ailments. Fucoxanthin (Fx), natural carotenoid known for its antioxidant capabilities, has shown promise alleviating renal injury, but exact mechanisms are yet to be fully understood. Carbon tetrachloride (CCl4) is recognized as powerful nephrotoxic substance, and this study explores the therapeutic effects Fx on oxidative stress,...
To compare the difference in forefoot width between minimally invasive extra-articular osteotomy via small incision and traditional Chevron treatment of hallux valgus. A retrospective analysis was conducted on clinical data 45 patients with valgus April 2019 July 2022. Among them, 22 cases underwent (minimally group), 23 (traditional group). There no significant baseline two groups ( P>0.05), including gender, age, affected side, Mann classification valgus, disease duration, preoperative...
To investigate the effectiveness of spring ligament repair combined with subtalar arthroereisis (STA) and Kidner procedure for treating children's flexible flatfoot painful accessory navicular. A retrospective analysis was conducted on clinical data from 45 children (45 feet) aged 7-14 years navicular who met selection criteria were treated between February 2018 May 2022. Among them, 23 cases (23 STA (observation group), while 22 (22 received alone (control group). Comparison baseline two...
This study aimed to explore the molecular mechanism of rubioncolin C(RuC) in inhibiting gastric cancer(GC). AGS and MGC803 cell lines were selected as cellular models. After treating cells with RuC at different concentrations, effects on proliferation ability GC assessed using CCK-8 method, real-time analysis(RTCA), colony formation assays. Transmission electron microscopy was used observe subcellular structural changes. Immunofluorescence applied detect LC3 fluorescent foci. Acridine orange...
Cell differentiation is determined by a certain set of transcription factors such as MyoD in myogenesis. However, that play positive role phenotypic gene expression skeletal cells are largely unknown, except the recently identified CBFA1. Scleraxis helix-loop-helix-type factor whose transcripts expressed sclerotome and cells; however, nothing known about its function with regard to regulation cell function. To examine possible roles scleraxis, we overexpressed scleraxis osteoblastic...
Abstract Background Chromatin-associated histone H2AX is a key regulator of the cellular responses to DNA damage. However, non-nucleosomal functions are poorly characterized. We have recently shown that soluble can trigger apoptosis but mechanisms leading non-chromatin-associated unclear. Here, we tested whether stalling replication, common event in cancer cells and underlying mechanism various chemotherapeutic agents, increased H2AX. Results Transient overexpression was found lead...