To explore the exact interaction between Notch and transforming growth factor (TGF)-β signaling in liver fibrosis.We established a rat model of fibrosis induced by concanavalin A. Peripheral blood mononuclear cells (PBMCs) were isolated from modeled rats, cultured with γ-secretase inhibitor DAPT TGF-β for 24 h. The mRNA levels detected quantitative real-time polymerase chain reaction. Expression proteins was analyzed western blotting.Compared to control activated PBMCs rats. Administration...

Growing evidence indicates that gut microbiota is involved in the regulation of host's sex hormone levels, such as through interfering with metabolism intestine. However, if or its metabolites directly influence biosynthesis gonad remains largely unknown. Our previous study showed colistin, a narrow-spectrum antibiotic, can significantly downregulate serum testosterone levels and thus enhance antitumor efficiency anti-PD-L1 male mice; however, underlying mechanism for uninvestigated. In...

Halofuginone (HF) is an active component of extracts derived from the plant alkaloid febrifugine and has shown therapeutic promise in animal models fibrotic disease. Our main objectives were to clarify suppressive effect HF on concanavalin A (ConA)-induced liver fibrosis. ConA injection into tail vein caused a great increase serum aspartate aminotransferase (AST) alanine (ALT) levels, while orally administration significantly decreased levels transaminases. In addition, hyaluronic acid (HA),...

Neural precursor cell-expressed, developmentally-downregulated 9 (NEDD9) is a multi-domain skeleton protein that serves an important role in the cell signaling process via modulating invasion, metastasis, proliferation and apoptosis of tumor cells. The present study identified expression levels NEDD9 colorectal cancer were elevated. Therefore, effect downregulating terms invasion migration cells was investigated JNK pathway these processes also investigated. data revealed downregulation...

: We prepared the sirolimus liposome by rapid expansion of supercritical solution (RESS) technology fluid, and studied effects temperature, pressure, equilibrium time on average particle size envelop rate liposome. The conditions minimal were 328K temperature (35MPa), 35MPa pressure (333K), 50 minutes (343K) time, respectively. maximal 333K (30MPa), (343K), (323K)

Background: MicroRNAs (miRNAs) are non-coding small RNAs that have been shown to play a key role in the development of many tumors. However, its specific mechanism action non-small cell lung cancer (NSCLC) is not very clear. Purpose: This study was identify effect miRNA-449a on NSCLC invasion and migration. Methods: We used quantitative real-time PCR experiments demonstrate down-regulated tissues lines. also Transwell assay detect migration, Western Blot protein expression. The dual...

Anti-inflammation strategy is one of the proposed therapeutic approaches to hepatic fibrosis. T helper (Th) 17 cells, which play a detrimental role in experimental murine models inflammatory diseases, have been demonstrated participate pathogenesis liver damage. The inhibitory effect halofuginone (HF), an active component extracts derived from plant alkaloid febrifugine, on collagen synthesis has shown animal fibrotic disease. aim this study was clarify vivo HF Th17 cells concanavalin...

: We investigated the neuroprotective effects of Rg2 in anoxic cultured hippocampal neurons newborn rats. The cells were divided into a control group, nimodipine group (5 μmol/L), (0.025 mmol/L), and (0.05 mmol/L) group. apoptosis rate was measured by flow cytometry with staining PI. intracellular calcium ion [Ca2+]i observed fluorospectrophotometer determined fluorescent probe Fluo-2/AM. contents MDA NO activities SOD supernatants biochemical methods. results demonstrated that reduced...

Spinocerebellar ataxia type 6 is a late onset autosomal dominantly inherited ataxic disorder, and previous patho‐anatomical studies have only reported neurodegeneration in SCA6 as being confined to the cerebellar cortex, dentate nucleus inferior olive. However, characteristics of symptoms many poorly understood “extracerebellar” reveal three regions corresponding precerebellar nuclei may undergo differing evolution degenerative process, more widespread brainstem degeneration SCA6. We carried...

Background/Aims: Epithelial-mesenchymal transition (EMT) is recognized as a crucial mechanism in breast cancer progression and metastasis. Paired-related homeobox 2 (Prrx2) has been identified new EMT inducer cancer, but the underlying mechanisms are still poorly understood. Methods: The expression of Prrx2 was assessed by immunohistochemistry tissues to evaluate clinicopathological significance Prrx2, well correlation between EMT. Short hairpin RNA knockdown used examine cellular effects...

Purpose: To explore the effect of miR-449a inhibits migration and invasion by targeting Notch1 regulating epithelial–mesenchymal transition (EMT) in hepatocellular carcinoma (HCC), further study on molecular mechanism. Patients methods: The expression HCC cells tissues was detected qRT-PCR. cell line HCCLM3 SMMC-7721 were transfected with miR-449a. lines transwell assay wound healing assay. Notch pathway EMT related protein Western Blotting. specific binding site mir-449a notch1 gene...

<p indent="0mm">Osteosarcoma (OS) is a primary bone cancer mostly found in adolescents and elderly individuals. The treatment of OS still largely dependent on traditional chemotherapy. However, the high incidence drug resistance remains one greatest impediments to limiting improvements treatment. Recent findings have indicated that transcription factor FOXM1 plays an important role various cancer-related events, especially resistance. possible methotrexate (MTX) be explored. Here, we find...

:To investigate the possible association between V249I and T280M polymorphisms carotid atherosclerosis (CAS) in a Han population northern China we studied 328 patients with increased artery intima-media thickness (C-IMT) 292 healthy controls normal C-IMT. polymorphic genotypes of CX3CR1 were determined by polymerase chain reaction restriction fragment length polymorphism. Results showed that M280 allele frequency CAS group was significantly lower than controls. Logistic regression analysis...

The purpose of this study was to explore the significance neuregulin-1/ErbB signaling pathway and its effect on Sox10 expression in course differentiation mouse bone marrow mesenchymal stem cells into Schwann-like vitro.The experiment conducted with three groups-control, TAK 165, HRG-off. In control group, we used classical induction method adding β-ME, RA, FSK, b-FGF, PDGF, neuregulin (HRG); were collected 7th day. Using same basic protocol as specific ErbB2 inhibitor mubritinib (TAK 165)...

Abstract Objective There are several reports that suggest a significant role played by microRNAs (miRNAs) in cell invasion, metastasis, differentiation, and apoptosis lung cancers. miR-375 is one such miRNA has been detected variety of tumors, but its specific activity non-small cancer (NSCLC) remains unclear. Methods In this study, we regulated the expression miR-375, to evaluate influence on 14-3-3ζ/ERK/MYC pathway NSCLC. Results The results our experiments 14-3-3ζ highly expressed NSCLC,...

To explore the expression change of genes related with matrix reconstitution during injury and murine bone marrow following treatment 5-fluorouracil (5-FU).C57BL/6 mice received intraperitoneal injection 5-FU (200 mg/kg), peripheral blood cell counts were monitored at 3, 6, 9, 15, 21, 27 days after treatment. Bone cells harvested these times for total RNA extraction using TRIzol. Reverse transcriptions in combination real-time PCR performed detecting reconstitution, including ECM-1, MMP-2,...

Objective: To investigate the role of Notch signaling pathway in differentiation rat bone marrow mesenchymal stem cells (MSCs) into Schwann (SCs). Methods: BMSCs were isolated from high glucose medium and cultured fetal bovine serum (FBS) α-MEM medium. The resulting treated with β-mercaptoethanol (β-ME), all-trans retinoic acid (RA), platelet-derived factor (PDGF-AA), basic fibroblast growth (bFGF), Forskolin, Heregulin .After induction differentiation,cells divided three groups: control...