A5036055279- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- RNA modifications and cancer
- Cancer therapeutics and mechanisms
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Genomics and Phylogenetic Studies
- Protein Structure and Dynamics
- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- Toxin Mechanisms and Immunotoxins
- Analytical Chemistry and Chromatography
- Monoclonal and Polyclonal Antibodies Research
- Pancreatic function and diabetes
- RNA Interference and Gene Delivery
- Chromosomal and Genetic Variations
Brown University
2004-2024
John Brown University
1975-2016
Harvard University
2004
Institute of Microbial Technology
2003
University of California, Los Angeles
2002
Southern Illinois University School of Medicine
2002
Stanford University
1997
MRC Laboratory of Molecular Biology
1967-1989
Medical Research Council
1967-1989
University of Southern California
1985
Protein-induced DNA bending is an important element in the structure of many protein-DNA complexes, including those involved replication, transcription, and recombination. To understand these structures, path followed by each complex must be established. We have generated empirical relation between degree altered electrophoretic mobility polyacrylamide gels that allows estimation protein-induced bends. This technique has been used to analyze 17 different complexes formed six proteins four...
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Lambda integrase is archetypic of site-specific recombinases that catalyze intermolecular DNA rearrangements without energetic input. cleavage, strand exchange, and religation steps are linked by a covalent phosphotyrosine intermediate in which Tyr 342 attached to the 3-phosphate cut site. The 1.9 angstrom crystal structure catalytic domain reveals protein fold conserved organisms ranging from archaebacteria yeast suggests model for interaction with target DNA. attacking nucleophile located...
High levels of covalent integrase-DNA complexes accumulate when suicide substrates containing a medial nick within the overlap region are nicked by lambda integrase protein. The tyrosine residue at position 342 is shown to form bond with DNA sites strand exchange. A mutant in which this changed phenylalanine devoid both topoisomerase and recombinase activity but still binds core- arm-type binding an affinity comparable wild-type integrase. Tyrosine-342 located 40-amino acid that conserved...
The multiprotein-DNA complexes that participate in bacteriophage lambda site-specific recombination were used to study the combined effect of protein-induced bending and protein-mediated looping DNA. protein integrase (Int) is a monomer with two autonomous DNA binding domains different sequence specificity. Stimulation Int cleavage at low affinity core-type sites required interactions high arm-type depended on simultaneous sequence-specific IHF (integration host factor). bivalent positioned...
establishment of lysogeny involves integration the circular X genome into bacterial chromosome by a sitespecific recombination event involving phage attachment site (designated attP or POP') and attB BOB').DNA sequence analyses ' A. Siege1 R. J. Deans, unpublished data.
INTRODUCTION Cells lysogenic for λ carry a quiescent form of the viral chromosome called prophage. The prophage differs from DNA particles in two important ways: (1) ends and particle are at different points nucleotide sequence, (2) covalently joined to host DNA. Campbell (1962) proposed that is converted by joining followed insertion resulting circular molecule into Insertion occurs reciprocal recombination specific sites (attachment or att sites) each (Fig. 1). This proposal has received...
The tyrosine tRNAs specified by the E. coli amber suppressor gene su III + and wild type non‐suppressing allele − were selectively labelled with 32 P in infected transducing phage ψ80 carrying either of these genes. determination nucleotide sequence tRNA is described. This has anticodon CUA. differs from a single where modified guanylic acid residue. cells contain two (I II). Species I identical to while species II nucleotides variable loop region.
The simple relation between the substrates and products of site-specific recombination raises questions about control directionality often observed in this class DNA transactions. For bacteriophage lambda, viral integration excision proceed by discrete pathways, with intrinsic property recombining only one direction can be constructed. These pathways display an asymmetric reliance on a complex array protein binding sites, they respond differently to changes concentrations relevant proteins....
The excisive recombination reaction of bacteriophage lambda involves a specific and efficient juxtaposition two distant higher order protein-DNA complexes on the chromosome Escherichia coli . These complexes, which mediate synapsis strand exchange, consist DNA sequences, att L R, bivalent binding protein Int, sequence-specific bending proteins, IHF, Xis, Fis. protein-protein interactions within, between, these were studied by various biochemical techniques patterns synergism among pairs...
Purified int protein from bacteriophage lambda binds to specific sites in DNA that are not part of the functional attachment (non-att DNA) as well att DNA. Analysis non-att protected nucleases by has permitted definition two distinctly different consensus recognition sequences, one which, arm-type sequence, is characterized this report. Both types sequence occur attP; five copies located distant crossover region P1, P2, and P' arm regions. The second type occurs at region. Modification with...
Reciprocal recombination between specific DNA sequences on the λ and E. coli chromosomes (attP attB, respectively) gives rise to left (attL) right (attR) prophage attachment (att) sites at junctures of an integrated viral genome (for reviews, see Gottesman Weisberg 1971; Nash 1977). All four these att are genetically distinguishable from one another; however, crossover occurs within (or boundaries of) a 15-bp “core region” that is common all them (Landy Ross The does not involve any...