A5115591056- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Advanced biosensing and bioanalysis techniques
- COVID-19 Clinical Research Studies
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Catalytic C–H Functionalization Methods
- Viral gastroenteritis research and epidemiology
- Cyclopropane Reaction Mechanisms
- Advanced Nanomaterials in Catalysis
- Catalytic Cross-Coupling Reactions
- Synthesis and Catalytic Reactions
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
- Protein Degradation and Inhibitors
- RNA and protein synthesis mechanisms
- CAR-T cell therapy research
- Sirtuins and Resveratrol in Medicine
- Polysaccharides and Plant Cell Walls
- HIV-related health complications and treatments
- Catalytic Alkyne Reactions
- Environmental Toxicology and Ecotoxicology
- Transgenic Plants and Applications
National Institutes for Food and Drug Control
2023-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
East China University of Science and Technology
2025
Quality Research
2025
First Institute of Oceanography
2024
Ministry of Natural Resources
2024
Guangdong Pharmaceutical University
2021-2024
Henan University
2024
China Banking Regulatory Commission
2024
Henan Agricultural University
2024
The recent outbreak of betacoronavirus Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which is responsible for the Disease 2019 (COVID-19) global pandemic, has created great challenges in viral diagnosis. existing methods nucleic acid detection are high sensitivity and specificity, but need complex sample manipulation expensive machinery slow down disease detection. Thus, there an urgent demand to develop a rapid, inexpensive, sensitive diagnostic test aid point-of-care...
The mRNA vaccine technologies have progressed rapidly in recent years. COVID-19 pandemic has accelerated the application of vaccines, with research and development clinical trials underway for many vaccines. Application quality by design (QbD) framework to establishing standardized control protocols vaccines are essential continued high-quality
An efficient and air-tolerant Ni-catalyzed denitrogenative cross-electrophile coupling of benzotriazinones with benzyl chlorides has been developed via liquid-assisted grinding by using Mn powders as reductant DMF assisting liquid in the presence anhydrous calcium chloride. Scope limitations protocol to access diarylmethanes have demonstrated more than 20 examples, showing acceptable tolerance functional group steric hindrance. Although electron-withdrawing substituents on benzotriazinone or...
Mucosal immunity is crucial for preventing the infection and transmission of respiratory viruses. Nasal antibody inversely correlated with a lower risk However, current reference standard nasal assessment serum-based, mainly consisting monomeric IgG IgA. The applicability serum-derived standards assessing antibodies, mostly dimeric or polymeric secretory IgA (sIgA), remains unvalidated. Herein, we first proved that sera-derived was not applicable antibodies. Using non-homologous as...
Cadmium (Cd) pollution has become a global issue due to industrial and agricultural developments. However, the molecular mechanism of Cd-induced detrimental effects relevant signal transduction/metabolic networks are largely unknown in marine fishes. Here, greenfin horse-faced filefish (Thamnaconus septentrionalis) were exposed 5.0 mg/L Cd up 7 days. We applied both biochemical methods multi-omics techniques investigate how gills respond exposure. Our findings revealed that exposure caused...
An indazolone-directed chemoselective synthesis of 8 H -indazolo [1,2- a ]cinnolines has been realized via cascade Cp*Ir( iii )- and Cp*Rh( )-catalyzed C–H activation/cyclization reaction 1-arylindazolones with sulfoxonium ylides.
Following the coronavirus disease pandemic, respiratory mucosal vaccines that elicit both and systemic immune responses have garnered increasing attention. However, human physiological characteristics pose significant challenges in evaluation of immunity, which directly impedes development application vaccines.
HIV-1 transmembrane protein gp41 plays a crucial role by forming stable six-helix bundle during HIV entry. Due to highly conserved sequence of gp41, the development an effective and safe small-molecule compound targeting is good choice. Currently, natural polyanionic ingredients with anti-HIV activities have aroused concern. Here, we first discovered that glycosylated dihydrochalcone, trilobatin, exhibited broad anti-HIV-1 activity low cytotoxicity in vitro. Site-directed mutagenesis...
Background/Objectives: Enterovirus 71 (EV71) and coxsackieviruses A16 (CA16), A10 (CA10), A6 (CA6) are the primary pathogens that cause hand, foot, mouth disease (HFMD). Currently, many manufacturers developing bivalent, trivalent, tetravalent vaccines target these antigens. Cell-based neutralization assay (CBNA), gold standard for detecting neutralizing antibodies (NtAbs), which used as indicators of HFMD vaccine efficacy, has several limitations. We aimed to develop a novel NtAbs against...
We conducted a multicenter, partially randomized, platform trial to assess the effectiveness of booster dose an aerosolized or intramuscular adenovirus type 5 vectored COVID-19 vaccine (Ad5-nCoV) in Chinese adults (NCT05855408). Between May 23, 2023, and August 28, 4089 eligible participants were equally randomized receive either Ad5-nCoV via oral inhalation at 0.1 mL (IH Ad5-nCoV, n = 2039) injection 0.5 (IM 2050). Additionally, 2008 who declined but consented participate surveillance...
Background: The three commercial Enterovirus 71 (EV71) inactivated vaccines which have effectively controlled the EV71 pandemic currently rely on inherent variable in vivo potency methods for batch release. To align with 3R (Replacement, Reduction, Refinement) principles and enhance quality control, this study referred to WHO guidelines European Pharmacopoeia develop vitro relative (IVRP) methods. Methods: Working standards tracing phase 3 clinical were established. Manufacture-specific IVRP...
Introduction Vaccines capable of effectively inducing mucosal immunity, particularly specific IgA antibodies, represent an ideal strategy for preventing infections and the transmission pathogens such as SARS-CoV-2 influenza viruses that rapidly replicate in upper respiratory tract cause clinical symptoms. However, a lack standardized nasal antibody detection sampling methods has hindered cross-study comparability vaccine development. Methods This study uses model pathogen to standardize...
An efficient Ni-catalyzed, Mn-mediated denitrogenative cross-electrophile coupling of N-alkyl-1,2,3-benzotriazinones with alkyl tosylates and mesylates for access to o-alkyl secondary benzamides is reported. The method uses inexpensive non-anhydrous dimethyl acetamide (DMA) in combination tetrabutyl ammonium iodide (TBAI) as a co-catalyst convert sulfonates into iodides situ. Scope limitations the protocol have been demonstrated by >30 examples yields up 91%, showing large electronic...
Potency is a critical quality attribute for controlling consistency and relevant biological properties of vaccines. Owing to the high demand animals, lengthy operations variability in vivo methods, vitro alternatives human vaccine potency assays are extensively developed.Herein, methods previously licensed vaccines were sorted, followed by brief description background substituting with alternatives. Based on analysis current research substitution assays, barriers suggestions proposed.Owing...
Semen is a critical vector for human immunodeficiency virus (HIV) sexual transmission and harbors seminal amyloid fibrils that can markedly enhance HIV infection. Semen-derived enhancer of viral infection (SEVI) one the best-characterized fibrils. Due to their highly cationic properties, SEVI capture virions, increase attachment target cells, augment fusion. Some studies have reported myricetin antagonizes β-protein (Aβ) formation; also displays strong anti-HIV activity in vitro. Here, we...
Neutralizing antibody (NtAb) levels are key indicators in the development and evaluation of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) vaccines. Establishing a unified reliable WHO International Standard (IS) for NtAb is crucial calibration harmonization detection assays. National other secondary standards links transfer IS to working but often overlooked. The Chinese (NS) were developed by China September December 2020, respectively, application which prompted coordinated...
The implementation of combined antiretroviral therapy (cART) has rendered HIV-1 infection clinically manageable and efficiently improves the quality life for patients with AIDS. However, persistence a latent reservoir is major obstacle to achieving cure A "shock kill" strategy aims reactivate HIV then kill it by immune system or cART drugs. To date, none LRA candidates yet demonstrated effectiveness in promising functional cure. Interestingly, phosphorylation activation antiapoptotic Bcl-2...
Neutralizing antibodies (NtAbs) against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) are indicators of vaccine efficacy that enable immunity surveillance. However, the rapid mutation SARS-CoV-2 variants prevents timely establishment standards required for effective XBB evaluation. Therefore, we prepared four candidate (No. 11, No. 44, 22, and 33) using plasma, purified immunoglobulin, a broad-spectrum neutralizing monoclonal antibody. Collaborative calibration was conducted...