A5115589618- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Antimicrobial Resistance in Staphylococcus
- Viral Infections and Immunology Research
- Salmonella and Campylobacter epidemiology
- Clostridium difficile and Clostridium perfringens research
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Streptococcal Infections and Treatments
- T-cell and B-cell Immunology
- Animal Disease Management and Epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Tuberculosis Research and Epidemiology
- Immune Response and Inflammation
- Mycobacterium research and diagnosis
- Cell Image Analysis Techniques
- Advanced machining processes and optimization
- Advanced Surface Polishing Techniques
- Toxin Mechanisms and Immunotoxins
- Listeria monocytogenes in Food Safety
- Vitamin C and Antioxidants Research
- Microbial infections and disease research
- RNA modifications and cancer
- Plant Virus Research Studies
Second Affiliated Hospital of Dalian Medical University
2025
Dalian Medical University
2025
Zhejiang Chinese Medical University
2024
State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems
2024
Lanzhou University
2024
Centre National de la Recherche Scientifique
2024
Guangxi Medical University
2024
Hubei University
2024
Henan Academy of Agricultural Sciences
2024
Anhui Agricultural University
2024
IOT refers to the third scientific and economic tide after computer, Internet in global information industry, having attracted highly attention of governments, enterprises, academia, brought a huge new market for communication industry. At present, main operators equipment suppliers begin provide M2M business solution. Here mainly introduces concept IOT, analyses structure IOT: perception layer, network application layer. Sets forth key technologies RFID, communication, etc. length, it...
Current super-resolution microscopy (SRM) methods suffer from an intrinsic complexity that might curtail their routine use in cell biology. We describe here random illumination (RIM) for live-cell imaging at super-resolutions matching of 3D structured microscopy, a robust fashion. Based on speckled and statistical image reconstruction, easy to implement user-friendly, RIM is unaffected by optical aberrations the excitation side, linear brightness, compatible with multicolor over extended...
We explored the use of a real-time cell analysis (RTCA) system for assessment Clostridium difficile toxins in human stool specimens by monitoring dynamic responses HS27 cells to tcdB toxins. The C. toxin caused cytotoxic effects on cells, which resulted dose-dependent and time-dependent decrease impedance. RTCA assay possessed an analytical sensitivity 0.2 ng/ml B with no cross-reactions other enterotoxins, nontoxigenic difficile, or Clostridum species. Clinical validation was performed 300...
Since the first isolation from human, astroviruses have been detected in many species. Wide host range and occasional cross-transmission of astrovirus pose a risk for zoonotic infection. Here, novel were identified goslings with recent epidemic gout disease China. A virus, designated as GD, was efficiently isolated diseased gosling using LMH cells. Genome GD amplified 5' 3' RACE 7183nt full length. Sequence analysis revealed genome <60.8% homology others deposited Genbank. Moreover, could be...
Porcine deltacoronavirus (PDCoV) is a pathogen belonging to the genus Deltacoronavirus that in 2014 caused outbreaks of piglet diarrhea United States. To identify suitable therapeutic targets, more comprehensive understanding viral entry pathway required, particularly role proteases. Here, we identified proteases activate spike (S) glycoprotein initiate cell and also pinpointed host-cellular pathways PDCoV uses for entry. Our results revealed cathepsin L (CTSL) B (CTSB) lysosomes...
Swine acute diarrhea syndrome coronavirus (SADS-CoV), a newly discovered enteric coronavirus, is the aetiological agent that causes severe clinical and intestinal pathological damage in piglets. To understand effect of SADS-CoV on host cells, we characterized apoptotic pathways elucidated mechanisms underlying process cell death after infection. SADS-CoV-infected cells showed evidence apoptosis vitro vivo. The use pan-caspase inhibitor resulted inhibition SADS-CoV-induced reduction...
Background. Methicillin-resistant Staphylococcus aureus (MRSA) has become a major health threat in the United States. Staphylococcal enterotoxin B (SEB) is potent superantigen that contributes to its virulence. High mortality and frequent failure of therapy despite available antibiotics have stimulated research efforts develop adjunctive therapies. Methods. Treatment benefits SEB-specific monoclonal antibody (mAb) 20B1 were investigated mice sepsis, superficial skin, deep-tissue infection...
Abstract Porcine epidemic diarrhea virus (PEDV) replicates in the cytoplasm of infected cells, but its nucleocapsid (N) protein localizes specifically to nucleolus. The mechanism nuclear translocation, and whether N associates with particular nucleolar components, is unknown. In this study, we confirm that a phosphoprotein nucleophosmin (NPM1) interacts co-localizes vitro binding studies indicated aa 148–294 118–188 NPM1 were required for binding. Interestingly, importation into nucleolus...
In this study, the pathogenicity of porcine deltacoronavirus (PDCoV) strain NH (passage 10, P10) was evaluated. We found that PDCoV is enteropathogenic in 5-day-old pigs. Pathogenicity experiments provided a challenge model for studying protection efficiency passive immunity. order to investigate protective efficacy immunity newborn piglets, pregnant sows were vaccinated with either PDCoV-inactivated vaccine at Houhai acupoint (n = 5) or DMEM as negative control 2) using prime/boost strategy...
To counteract the host antiviral defenses, most viruses, including coronaviruses, have evolved with diverse strategies to dampen IFN-mediated response, by interfering or evading specific regulators at multiple steps of this response. In study, a novel antagonistic strategy was revealed showing that PEDV infection could circumvent innate response targeted degradation endogenous FBXW7 in target cells, process verified be positive modulator for immune system.
This study profiled global single cell-spatial-bulk transcriptome landscapes of hepatocellular carcinoma (HCC) ecosystem from six HCC cases and a non-carcinoma liver control donor. We discovered that intratumoral heterogeneity mainly derived cells diversity pervaded the genome-transcriptome-proteome-metabolome network. are core driving force taming tumor-associated macrophages (TAMs) with pro-tumorigenic phenotypes for favor its dominant growth. Remarkably, M1-types TAMs had been...
We describe here the use of an immunomagnetic separation enrichment process coupled with a modified real-time cellular analysis (RTCA) system (RTCA version 2) for detection C. difficile toxin (CDT) in stool. The limit CDT by RTCA 2 was 0.12 ng/ml. Among consecutively collected 401 diarrheal stool specimens, 53 (13.2%) were toxin-producing strains quantitative toxigenic culture (qTC); bacterial loads ranged from 3.00 × 10(1) to 3.69 10(6) CFU/ml. method detected 51 samples, resulting...
Transmissible gastroenteritis virus (TGEV) is an infective coronavirus (CoV) that causes diarrhea-related morbidity and mortality in piglets. For the first time, a natural recombination strain of TGEV Anhui Hefei (AHHF) between Purdue Miller clusters was isolated from small intestine content piglets China. A phylogenetic tree based on complete genome sequence placed AHHF clusters. The results computational analysis showed recombinant these Two breakpoints located open reading frame 1a...
Background. Staphylococcal enterotoxin B (SEB), a potential biological warfare agent, is potent superantigen that contributes to the virulence of methicillin-resistant Staphylococcus aureus (MRSA), which major health threat in United States. Efforts develop toxin-neutralizing antibodies as adjunctive therapies are justified, given high mortality and frequent failure therapy despite available antibiotics. Methods. Murine SEB-specific mAb 20B1 was humanized, treatment benefits Hu-1.6/1.1...
Staphylococcal enterotoxin B (SEB) is a superantigen that cross-links the major histocompatibility complex class II and specific V-β chains of T-cell receptor, thus forming ternary complex. Developing neutralizing mAb to disrupt abrogate resulting toxicity therapeutic challenge because SEB effective at very low concentrations. We show combining two SEB-specific mAbs enhances their efficacy, even though one by itself has no effect on neutralization. Crystallography was employed for...
TGEV is the causative agent of porcine diarrhea, a viral disease that economically affects pig industry in many countries. However, molecular mechanisms underlying replication remain incompletely understood.
ABSTRACT Staphylococcal enterotoxin B (SEB) is a potent toxin that produced by Staphylococcus aureus strains and classified as category select agent. We have previously shown monoclonal antibody (MAb) 20B1, murine anti-SEB IgG1, successfully treats SEB-induced lethal shock (SEBILS) bacteremia caused SEB-producing S. . In this study, we generated two isotype switch variants of the original IgG1 MAb an IgG2a IgG2b, both bearing same variable region sequence, compared their neutralizing...