A5100386508- Autophagy in Disease and Therapy
- Sirtuins and Resveratrol in Medicine
- Toxin Mechanisms and Immunotoxins
- Viral Infections and Immunology Research
- Calcium signaling and nucleotide metabolism
- Genomics, phytochemicals, and oxidative stress
- Ubiquitin and proteasome pathways
- Ferroptosis and cancer prognosis
- Cell death mechanisms and regulation
- RNA modifications and cancer
- Extracellular vesicles in disease
- Herpesvirus Infections and Treatments
- Receptor Mechanisms and Signaling
- Nutrition and Health in Aging
- Ion Channels and Receptors
- Rheumatoid Arthritis Research and Therapies
- Helicobacter pylori-related gastroenterology studies
- Radioactivity and Radon Measurements
- Apelin-related biomedical research
- Advanced Glycation End Products research
- Phytochemistry and Biological Activities
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Animal Disease Management and Epidemiology
- ATP Synthase and ATPases Research
Center of Hubei Cooperative Innovation for Emissions Trading System
2024
Shanghai University of Medicine and Health Sciences
2024
Anhui Medical University
2014-2024
Huazhong Agricultural University
2022-2024
Sichuan Cancer Hospital
2024
University of Electronic Science and Technology of China
2024
Jingzhou Central Hospital
2024
Yangtze University
2024
Shanghai Sixth People's Hospital
2019-2024
Shanghai Jiao Tong University
2019-2024
Abstract The present study was designed to explore the biological role of resveratrol (RES) in rheumatoid arthritis (RA) and underlying mechanism. adjuvant‐induced arthritic rats were administered RES on 12th day after model establishment, then assessment, oxidative stress measurement, histological examination, immunohistochemical staining performed. primary rat fibroblast‐like synoviocytes (FLS) isolated treated with vitro cell proliferation apoptosis assay examined. Chromatin...
Resveratrol (Res) is a polyphenolic compound that has variety of biological functions and activities. This study aimed to explore the mechanisms antioxidant proapoptotic effects Res in H2 O2 -treated rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs) by Nrf2-Keap1 signaling pathway. We found 5 µM promoted cell proliferation increased intracellular reactive oxygen species (ROS) malondialdehyde (MDA) content RA-FLSs. However, could reverse these µMH2O2-treated RA-FLSs (1) promoting...
Abstract Abnormal antioxidative capabilities were observed in the pathogenesis of steroid-induced osteoporosis (SIOP). Ferroptosis is a recently discovered type cell death that characterized by overproduction ROS response to GPX4 and system X c− downregulation, which mediated an Fe 2+ fenton reaction. However, investigations focusing on relationship between ferroptosis bone disease remain limited. In present study, high-dose dexamethasone was used establish mouse SIOP model, extracellular...
There is growing concern that chemotherapy drugs can damage Leydig cells and inhibit the production of testosterone. Increasing evidence shows melatonin benefits reproductive process. This study mainly explores protective effect possible molecular mechanism regarding cisplatin-induced oxidative stress in testicular tissue cells. We found there were only Sertoli testes gastrointestinal tumor patients with azoospermia caused by platinum chemotherapeutic drugs. Melatonin (Mel) receptor...
Rheumatoid arthritis (RA) is a chronic inflammatory disease with unknown etiology. The present study investigated the anti‑inflammatory effect of resveratrol on rats adjuvant (AA) abnormal immunological function via reduction cyclooxygenase‑2 (COX‑2) and prostaglandin E2 (PGE2). AA model were established by injection complete Freund's alterations in secondary paw swelling polyarthritic scores observed. Pathological examination joint tissues was observed hematoxylin eosin staining....
Abstract Resveratrol is a polyphenol derivatives which exhibits pro-apoptotic effect in variety of human cancers by triggering mitochondria apoptosis pathway and autophagy. However, there are scarcely reports on its apoptosis-promoting abnormal proliferation fibroblast-like synoviocytes (FLSs). In this study, we investigated the underlying mechanism apoptosis-inducing effects resveratrol FLSs adjuvant-arthritis (AA) rats. Since using for 12 days resulted significant decreasing swelling...
Glioblastoma is one of the most common and lethal intracranial malignant, still lack ideal treatments. Kaempferol a major nutrient found in various edible plants, which has exhibited potential for treatment glioblastoma. However, specific anti-glioma mechanism kaempferol yet to be studied. Herein, we aim explore mechanisms underlying activity kaempferol. Our results demonstrated that suppresses glioma cell proliferation vitro inhibits tumor growth vivo . Moreover, raises ROS decreases...
Adrenergic receptors are highly homologous while at the same time display a wide diversity of ligand and G-protein binding, understanding this is key for designing selective or biased drugs them. Here, we determine two crystal structures α2A adrenergic receptor (α2AAR) in complex with partial agonist an antagonist. Key non-conserved residues from ligand-binding pocket (Phe7.39 Tyr6.55) to coupling region (Ile34.51 Lys34.56) discovered play role interplay between agonism signaling α2AAR,...
Abstract Background Untargeted metabolomics can be used to expand our understanding of the pathogenesis sarcopenia. However, metabolic signatures sarcopenia patients have not been thoroughly investigated. Herein, we explored metabolites associated with by untargeted gas chromatography (GC)/liquid (LC)–mass spectrometry (MS) and identified possible diagnostic markers. Methods Forty-eight elderly subjects were age sex matched 48 without We first GC/LC–MS analyze plasma these participants then...
Apelin and Elabela are endogenous peptide ligands for receptor (APJ), a widely expressed G protein-coupled receptor. They constitute spatiotemporal dual ligand system to control APJ signal transduction function. We investigated the effects of Apelin-13, pGlu 1 -apelin-13, Apelin-17, Apelin-36, Elabela-21 Elabela-32 peptides on transduction. Whether different biased mediated pathways was studied. observed changes protein dependent β-arrestin signaling after activated by six ligands....
Background Local brain tissue can suffer from ischaemia/reperfusion (I/R) injury, which lead to vascular endothelial damage. The peptide δ opioid receptor (δOR) agonist [D-ala2, D-leu5]-Enkephalin (DADLE) reduce apoptosis caused by acute I/R injury in microvascular cells (BMECs). Objective This study aims explore the mechanism DADLE enhances level of mitophagy BMECs upregulating expression transient potential vanilloid subtype 4 (TRPV4). Methods were extracted and made undergo oxygen-glucose...
The dysfunction of articular chondrocytes is a crucial step in rheumatoid arthritis (RA) pathogenesis while its molecular mechanisms are not fully known. This study was aimed to investigate the expression aquaporin 4 (AQP4) adjuvant-induced (AIA) rats and involvement AIA development.Thirty were divided into normal group (n = 15). Rat induced by intradermal injection complete Freund's adjuvant evaluated secondary paw swelling histological assessments on knee joint damage. Localization protein...
Apoptosis and autophagy mutually regulate various cellular physiological pathological processes. The crosstalk between apoptosis is multifaceted complicated. Elucidating the molecular mechanism of their will advance therapeutic applications for treating cancer other diseases. NOXA, a BH3-only member BCL-2 family, was reported to induce promote autophagy. Here, we report that regulates by targeting NOXA degradation. Inhibiting increases protein levels extending half-life. accumulation...
Resveratrol, a bioactive compound predominantly found in grapes and red wine, provides wide range of properties that are beneficial for health, including anticancer anti‑inflammatory activities. Previously published studies have addressed the potential therapeutic effects resveratrol on rheumatoid arthritis (RA); however, subcellular mechanism remains to be fully elucidated. In present study, adjuvant (AA) Sprague‑Dawley rats were investigated, mechanisms resveratrol‑induced apoptosis...
Bisphenol A (BPA) is a chemical used in numerous consumer products that able to interfere with the mammalian endocrine system. The aim of present study was investigate effects BPA on male mouse reproductive cells following prenatal postnatal exposure. In addition, influence detected expression levels β‑catenin and dickkopf WNT signaling pathway inhibitor 1 (DKK‑1) during differentiation spermatogenic testes. DKK‑1 are two important proteins Wnt/β‑catenin pathway. On gestational day 1,...
Our previous study demonstrated that hyperbaric oxygen (HBO) improves heart function predominantly through reducing stress, modulating energy metabolism and inhibiting cell apoptosis. The present aimed to investigate the protective effects of HBO on mitochondrial autophagy using rats with a ligated left anterior descending artery. cardioprotective were mainly evaluated ELISA, fluorescent probes, transmission electron microscopy reverse transcription‑quantitative PCR (RT‑qPCR). pretreatment...
Src homology-2 domain containing protein tyrosine phosphatase-2 (SHP2) is a highly attractive therapeutic target for treating Kirsten rat sarcoma viral oncogene (KRAS) mutant cancers. In this work, series of guanidine-based SHP2 allosteric inhibitors were discovered via virtual screening and rational structural optimization. Notably, lead compound 23 with potent inhibitory activity (IC50 = 17.7 nM) effectively inhibited the proliferation, migration, invasion MIA PaCa-2 pancreatic cancer...
Abstract Background The pathogenesis of connective tissue disease‐associated interstitial lung disease (CTD‐ILD) is unclear. This study aims to identify differentially expressed proteins (DEPs) in CTD‐ILD determine the potential role these DEPs that may play and offer therapeutic targets. Methods Bronchoalveolar lavage fluid (BALF) samples were collected from four patients with without CTD‐ILD. Label‐free mass spectrometry‐based relative quantification was used DEPs. Bioinformatics...