A5101661553- Tuberculosis Research and Epidemiology
- Single-cell and spatial transcriptomics
- Nanoplatforms for cancer theranostics
- SARS-CoV-2 detection and testing
- Molecular Biology Techniques and Applications
- Cancer Research and Treatments
- Infectious Encephalopathies and Encephalitis
- Ocular Diseases and Behçet’s Syndrome
- Mycobacterium research and diagnosis
- Biochemical and Molecular Research
- Immune cells in cancer
- Salmonella and Campylobacter epidemiology
- COVID-19 Clinical Research Studies
- Advanced Biosensing Techniques and Applications
- Tryptophan and brain disorders
- Pneumocystis jirovecii pneumonia detection and treatment
- Intensive Care Unit Cognitive Disorders
- Bacteriophages and microbial interactions
- HIV/AIDS drug development and treatment
- Cancer therapeutics and mechanisms
- Global Maternal and Child Health
- Child and Adolescent Health
- Long-Term Effects of COVID-19
- Pancreatic and Hepatic Oncology Research
- Listeria monocytogenes in Food Safety
Bruker (United States)
2025
Bruker (Canada)
2025
Bruker (Switzerland)
2024
University of Southern California
2022-2024
Nanostring Technologies (United States)
2022-2023
Institute for Systems Biology
2021-2022
McMaster University
2022
Impact
2022
InSysBio (Russia)
2021
Merck & Co., Inc., Rahway, NJ, USA (United States)
2018
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), is the leading cause of death worldwide infectious disease. Treatment Mtb infection requires a six-month course multiple antibiotics, an extremely challenging regimen necessitated Mtb's ability to form drug-tolerant persister cells. formation dependent on trehalose catalytic shift, stress-responsive metabolic remodeling mechanism in which disaccharide liberated from cell surface glycolipids and repurposed as internal carbon...
Abstract Doravirine is a nonnucleoside reverse transcriptase inhibitor in clinical development for the treatment of human immunodeficiency virus‐1 infection combination with other antiretroviral therapies. The cytochrome P450 (CYP)3A‐dependent metabolism doravirine makes it susceptible to interactions modulators this pathway, including antituberculosis rifampin. Rifabutin, an alternative antibiotic used treat tuberculosis, may have lower‐magnitude effect on CYP3A. aim trial was determine...
Analyses of patient-derived cell lines have greatly enhanced discovery molecular biomarkers and therapeutic targets. However, characterization cellular morphological properties is limited. We studied morphologies human pancreatic adenocarcinoma (PDAC) their associations with drug sensitivity, gene expression, functional properties. By integrating live spatial mRNA imaging, we identified KRAS inhibitor-induced changes specific for drug-resistant cells that correlated expression changes. then...
Abstract Cancer research has long sought to understand the complex tumor microenvironment and molecular underpinnings of progression. Traditional methods often fail capture spatial organization cell-cell interactions within tumors. The CosMx® Spatial Molecular Imager (SMI) with its CosMx Whole Transcriptome (WTX) panel offers a transformative solution, providing spatially resolved, single-cell transcriptomics nanometer-scale precision. This platform enables mapping RNA molecules their native...
Single-cell RNA-seq revolutionized single-cell biology, by providing a complete whole transcriptome view of individual cells. Regrettably, this was accomplished only for individual, tissue-dissociated High-plex spatial biology has begun to recover the x, y, and z-coordinates single-cells, but typically at expense far less than coverage. To solve problem, Bruker Spatial Biology commercial-grade panel (CosMx® Molecular Imager Whole Transcriptome Panel (WTx)), using 37,872 imaging barcodes,...
Abstract Patient response to immunotherapy has been revolutionary but remains limited due the inability convert excluded or cold tumors into ones which would be permissive therapeutic intervention. To treat patients that evade immune therapy, comprehensive understanding of their tumor microenvironment (TME) is needed. date, most profiling efforts have lacked ability capture high-plex ‘omics data while retaining spatial architecture TME. We developed CosMx™ Spatial Molecular Imager (SMI) for...
Abstract Tuberculosis (TB) is a leading cause of death among infectious diseases worldwide due to latent TB infection, which the critical step for successful pathogenic cycle. In this stage, Mycobacterium tuberculosis resides inside host in dormant and antibiotic-tolerant state. Latent infection can lead multisystemic because M. invades virtually all organs, including ocular tissues. Ocular (OTB) occurs when bacilli within tissues reactivate, originally seeded by hematogenous spread from...
ABSTRACT Tuberculosis (TB) is a leading cause of death among infectious diseases worldwide due to latent TB infection, which the critical step for successful pathogenic cycle. In this stage , Mycobacterium tuberculosis resides inside host in dormant and antibiotic-tolerant state. Latent infection can also lead multisystemic because M. invades virtually all organs, including ocular tissues. Ocular (OTB) occurs when bacilli within tissues reactivate, originally seeded by hematogenous spread...
Abstract The immunological picture of how different patients recover from COVID-19, and those recovery trajectories are influenced by infection severity, remain unclear. We investigated 140 COVID-19 diagnosis to convalescence using clinical data, viral load assessments, multi-omic analyses blood plasma circulating immune cells. Immune-phenotype dynamics resolved four trajectories. One trajectory signals a return pre-infection healthy baseline, while the other three characterized differing...
Background: Safe, effective, inexpensive treatment for COVID-19 is an urgent unmet medical need. Zinc and resveratrol have been reported to antiviral activity may increase zinc at the site of replication by increasing intracellular concentrations.Methods: A 1:1 randomized, placebo-controlled trial 150 mg plus 4 grams daily 5 days versus placebos in SARS-CoV-2 positive outpatients was carried out 9/21/2020 – 1/22/2021 Seattle, Washington. Patients were enrolled within four testing if they had...
Background: Safe, effective, and inexpensive treatment for COVID-19 is an urgent unmet medical need. Zinc resveratrol have been reported to antiviral activity, may increase zinc activity at the site of replication by increasing intracellular concentrations. Methods: A 1:1 randomized, placebo-controlled trial 150 mg plus 4 g daily 5 days versus placebos in outpatients with SARS-CoV-2 was carried out from 9/21/2020–1/22/2021 Seattle, Washington. Viral shedding followed patient self-collected...
<h3>Background</h3> Patient response to immunotherapy has been revolutionary but remains limited due the inability convert excluded or cold tumors into ones which would be permissive therapeutic intervention. To treat patients that evade immune therapy, comprehensive understanding of their tumor microenvironment (TME) is needed. date, most profiling efforts have lacked ability capture high-plex 'omics data while retaining spatial architecture TME. We developed CosMx™ Spatial Molecular Imager...