A5000096233- Barrier Structure and Function Studies
- Aldose Reductase and Taurine
- Ion Transport and Channel Regulation
- Chronic Kidney Disease and Diabetes
- Renal Diseases and Glomerulopathies
- Neuroinflammation and Neurodegeneration Mechanisms
- Drug Transport and Resistance Mechanisms
- Metabolism and Genetic Disorders
- Electrolyte and hormonal disorders
- Pancreatic function and diabetes
- Potassium and Related Disorders
- Muscle metabolism and nutrition
- Pharmacological Effects and Toxicity Studies
- Prion Diseases and Protein Misfolding
- Neurological Disease Mechanisms and Treatments
- Aortic aneurysm repair treatments
- Acute Ischemic Stroke Management
- Blood Pressure and Hypertension Studies
- Dialysis and Renal Disease Management
- Renal function and acid-base balance
- Neuroscience and Neuropharmacology Research
- Kidney Stones and Urolithiasis Treatments
- Sodium Intake and Health
- Nicotinic Acetylcholine Receptors Study
- Epilepsy research and treatment
Osaka Rosai Hospital
2014-2025
Fukuoka University
2015-2024
Kitano Hospital
2016
Asahikawa University
2014-2015
Ibaraki Prefectural Central Hospital
2013
Fukuoka University Hospital
2004-2011
Saitama Medical University
2010
Social Insurance Saitama Chuo Hospital
2010
Center for Rheumatology
2007
Aichi Medical University
2006
Many hypertonic bacteria, plants, marine animals, and the mammalian renal medulla are protected from deleterious effects of high intracellular concentrations electrolytes by accumulating nonperturbing osmolyte betaine. When kidney-derived Madin-Darby canine kidney (MDCK) cells cultured in medium, they accumulate betaine to 1,000 times its medium concentration. This results induction hypertonicity rates transport into cells. We have isolated a cDNA (BGT-1) encoding transporter screening an...
Kidney medullary cells in situ, as well kidney-derived Madin-Darby canine kidney (MDCK) accumulate nonperturbing, small organic solutes (osmolytes), including myo-inositol, when bathed hypertonic media. Accumulation of osmolytes balances the osmolality extracellular fluid without raising intracellular salts that would perturb cellular functions. In media, increased myo-inositol accumulation is result activity a Na+/myo-inositol cotransporter. We have isolated cDNA encoding cotransporter from...
Abstract Background Increased matrix metalloproteinase (MMP)-9 in the plasma and brain is associated with blood-brain barrier (BBB) disruption through proteolytic activity neuroinflammatory diseases. MMP-9 present microvasculature its vicinity, where microvascular endothelial cells (BMECs), pericytes astrocytes constitute BBB. Little known about cellular source role of at Here, we examined ability to release migrate response inflammatory mediators comparison BMECs astrocytes, using primary...
The blood–brain barrier (BBB) is formed by brain endothelial cells. Many immortalized cell lines have been established; these used as in vitro BBB models. aim of the present study was to assess paracellular properties mouse bEND.3, bEND.5 cells, and 4 (MBEC4), those primary cells pMBECs. bEND.3 showed low permeability sodium fluorescein obvious staining tight junction proteins (claudin-5, occludin ZO-1) similar pMBECs; MBEC4 were low. In addition, expressed highest level claudin-5 among all...
Background. Tacrolimus is a substrate of P-glycoprotein (PGP) encoded by the multidrug resistant (MDR)1 gene (ABCB1). PGP, efflux pump, restricts distribution tacrolimus in brain. In this study, we investigate correlation ABCB1 polymorphism with tacrolimus-induced neurotoxicity patients after liver transplantation. Methods. The genotype 6 neurotoxic events and 11 without was analyzed polymerase chain reaction (PCR), 8 mutations were detected. addition to laboratory findings patient...
To assess whether an association between sodium-sensitive hypertension and metabolic syndrome exists; whether, in patients with syndrome, the nocturnal fall of blood pressure decreases salt restriction affects circadian rhythm.Japanese essential hypertension, who were treated without any antihypertensive agent, maintained on a high-sodium diet low-sodium for 1 week each. On last day each diet, 24-h pressures measured. A diagnosis was made according to International Diabetes Foundation...
Betaine is one of the major compatible osmolytes accumulated by kidney derived Madin-Darby canine cells cultured in hypertonic medium. Na(+)- and Cl(-)-dependent uptake from To gain insight into mechanism which hypertonicity evokes an increase Vmax betaine transporter cells, we measured relative abundance mRNA for shifted to a medium found parallel increases cotransporter activity. The levels preceded activity slightly. Transcription gene rose rapidly same extent as medium, indicating that...
Madin-Darby canine kidney cells behave like the renal medulla and accumulate small organic solutes (osmolytes) in a hypertonic environment. The accumulation of osmolytes is primarily dependent on changes gene expression enzymes that synthesize (sorbitol) or transporters uptake them (myo-inositol, betaine, taurine). mechanism by which hypertonicity increases transcription these genes, however, remains unclear. Recently, it has been reported yeast mitogen-activated protein (MAP) kinase its...
Myo-inositol is a major compatible osmolyte accumulated in the hypertonic renal medulla and Madin-Darby canine kidney (MDCK) cells cultured media. taken up by MDCK on Na(+)-coupled transporter whose activity increases sixfold 24 h after are switched to medium. To investigate mechanism of regulation cotransporter hypertonicity, we used cDNA encoding Na(+)-myo-inositol that recently cloned measure abundance mRNA for its rate transcription changes osmolality. When were from isotonic medium,...
Several chemicals inhibit the accumulation of abnormal prion proteins in vitro. We administered one, antimalarial agent quinacrine, to three patients with sporadic Creutzfeldt-Jakob disease (CJD) and one iatrogenic CJD. Quinacrine at 300 mg/day was given enterally for 3 months. Within 2 weeks administration, arousal level patient akinetic mutism improved. The other patients, insensible before treatment, had integrative responses such as eye contact or voluntary movement response verbal...
Central nervous system (CNS) complications of human immunodeficiency virus (HIV) infection remain a serious health risk in HIV/acquired syndrome despite significant advances highly active antiretroviral therapy (HAART). Specific drugs used for HAART are substrates the efflux transport systems, such as multidrug resistance-associated proteins (MRPs), which present on brain microvascular endothelial cells (BMEC) and astrocytes, that is, main cell types form blood-brain barrier (BBB). Thus,...
The blood–brain barrier (BBB) restricts the entry of circulating drugs and xenobiotics into brain, thus its permeability to substances is a critical factor that determines their central effects. infant brain vulnerable neurotoxic partly due immature BBB. employment in vitro BBB models evaluate compounds provides higher throughput than vivo animal experiments. However, existing have not been able simulate intrinsic neonatal To establish model mimics age-related properties, adult were...