A5101471442- Dental materials and restorations
- Redox biology and oxidative stress
- Dental Research and COVID-19
- Carbohydrate Chemistry and Synthesis
- Endodontics and Root Canal Treatments
- Asymmetric Synthesis and Catalysis
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Sulfur Compounds in Biology
- Chemical Synthesis and Analysis
- Cyclopropane Reaction Mechanisms
- Dental Erosion and Treatment
- Synthetic Organic Chemistry Methods
- Viral Infections and Immunology Research
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Catalytic C–H Functionalization Methods
- Advanced biosensing and bioanalysis techniques
- Trace Elements in Health
- Microbial Applications in Construction Materials
- Glutathione Transferases and Polymorphisms
- Marine Sponges and Natural Products
- Dental Implant Techniques and Outcomes
- Ginger and Zingiberaceae research
- Synthesis of Organic Compounds
Scripps Research Institute
2021-2023
Hokkaido University
2004-2023
Scripps (United States)
2023
Kyoto Pharmaceutical University
2015-2020
Kyoto University
1997-2011
Health Sciences University of Hokkaido
2005
Tokyo University of Agriculture and Technology
2003-2004
Josai University
2003
Yamagata University
2002
Reprogramming known medicines for a novel target with activity and selectivity over the canonical is challenging. By studying binding interactions between RNA folds small-molecule mining resultant dataset across human RNAs, we identified that Dovitinib, receptor tyrosine kinase (RTK) inhibitor, binds precursor to microRNA-21 (pre-miR-21). Dovitinib was rationally reprogrammed pre-miR-21 by using it as an recognition element in chimeric compound also recruits RNase L induce RNA's catalytic...
Ribonuclease targeting chimeras (RiboTACs) induce degradation of an RNA target by facilitating interaction between and a ribonuclease (RNase). We describe the screening DNA-encoded library (DEL) to identify binders monomeric RNase L provide compound that induced dimerization L, activating its activity. This was incorporated into design next-generation RiboTAC targeted microRNA-21 (miR-21) precursor alleviated miR-21-associated cellular phenotype in triple-negative breast cancer cells. The...
This study examined the surface staining mechanism of a photopolymerized composite by coffee, oolong tea, and red wine. Dental was subjected to an experimental 24-hour cycle: 17-hour immersion in artificial saliva solution containing 0.3% mucin followed 7-hour or After one, two, four weeks, digital images were analyzed grayscale mode with imaging analyzer. Specimens polished but not immersed used as baseline measurement for color change. Additionally, effects mechanical brushing...
The purpose of this study was to evaluate the long‐term durability in vivo bond strengths and morphological changes interfaces between dentin two adhesive systems. Class V preparations were prepared on facial surfaces 14 intact teeth monkeys restored with a combination Unifil Bond/Z250 or Single Bond/Z250. One year later, 10 additional same materials killed after 24 h. All subjected microtensile strength ( µ TBS) testing. debonded sides morphologically observed using Fe‐scanning electron...
A solid-phase DNA-encoded library (DEL) was studied for binding the RNA repeat expansion r(CUG)exp, causative agent of most common form adult-onset muscular dystrophy, myotonic dystrophy type 1 (DM1). variety uncharged and novel binders were identified to selectively bind r(CUG)exp by using a two-color flow cytometry screen. The cellular activity one binder augmented attaching it with module that directly cleaves r(CUG)exp. In DM1 patient-derived muscle cells, compound specifically bound...
Mitochondria play a central role in the initiation of apoptosis, which is regulated by various factors such as ATP synthesis, reactive oxygen species, redox status, and outer membrane permeabilization. Disruption chicken thioredoxin 2 (Trx2), mitochondrial redox-regulating protein, results apoptosis DT40 cells. To investigate mechanism this we prepared transfectants expressing control (DT40-TRX2-/-), human (TRX2) (DT40-hTRX2), or redox-inactive TRX2 (DT40-hTRX2CS) conditional Trx2-deficient...
Abstract Aim To evaluate the dental pulp response to a novel mineral trioxide aggregate containing phosphorylated pullulan (MTAPPL) in rats after direct capping. Methods Ninety‐six cavities were prepared maxillary first molars of 56 male Wistar rats. The pulps intentionally exposed and randomly divided into four groups according application capping materials: MTAPPL; (PPL); conventional MTA (Nex‐Cem MTA, NCMTA; positive control); Super‐Bond (SB; negative control). All restored with SB...
A catalytic domino reaction producing substituted 2-alkylidenecyclohexanone from 3-oxymethyl-2-siloxy-1,3-butadienes, which can be prepared Baylis–Hillman adducts, and α,β-unsaturated ketones is described. The process involves two mechanistically distinct reactions, (4+2) cycloaddition elimination. Both of these reactions are catalyzed by Tf2NH.
Protein-targeted small molecule medicines often bind RNAs and affect RNA-mediated pathways in cells. Historically, engagement modulation of RNA have not been considered medicine development; however, should be both a potential on- off-target. Kinase inhibitors emecrged as common binders with dovitinib, classic receptor tyrosine kinase (RTK) inhibitor, inhibiting RTKs the biogenesis oncogenic microRNA-21 through direct engagement. In this study, we use knowledge molecular recognition protein...
The micro-shear bond strengths (MSBSs) of five single-step self-etch adhesives (Adper Prompt L-Pop [APL], AQ Bond plus [AQP], OBF-2 [OB2], Reactmer [RB], and Xeno III [XIII]) were compared with that a two-step adhesive, Clearfil SE [SE]. applied on dentin surfaces, according to manufacturers' instructions, for bonding resin composite dentin. After 24 hours, test was carried out the data analyzed by oneway ANOVA Bonferroni (p<0.05). mean MSBSs in MPa APL: 22.8, AQP: 37.4, OB2: 34.7, RB: 28.3,...
To morphologically evaluate the interface between a conventional glass-ionomer cement (GIC) and dentin one day after placement, as well changes at year of aging/functioning in monkey teeth.On buccal surfaces seven intact teeth each two monkeys, shallow class V cavities were prepared, which then filled with Fuji IX GP (GC) to provide 1-year vivo data. A later, more similarly prepared restored for 1-day group. The following day, extracted restoration interfaces observed using transmission...
Novel peptide mimetics of GDP-fucose were designed and synthesized targeting inhibitors the fucosyltransferases that transfer l-fucose from to oligosaccharides, on basis background nikkomycin Z, a mimetic UDP-N-acetylglucosamine, shows potent inhibitory activity toward an N-acetylglucosamine enzyme. The synthetic routes take advantage enzymatic aldol reaction catalyzed by l-threonine aldolase prepare guanine carrying β-hydroxy-α-l-amino acid, key intermediate.
Novel mimics of cytidine 5'-monophosphate-sialic acid (CMP-sialic acid) were designed and synthesized for targeting inhibitors sialyltransferases on the basis phenomenon that tautomerization 5-fluorouracil from lactam to lactim form produces a structure similar cytosine, C-terminal's peptide bond can be bioisoster phosphate group.Since γ-N 1 -(5-fluorouracilyl)-β-hydroxy-α-L-amino acid, key synthetic intermediate, was easily prepared using enzyme-catalyzed aldol reaction, synthesis...