A5024416676- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Cancer-related gene regulation
- Prostate Cancer Diagnosis and Treatment
- Eosinophilic Disorders and Syndromes
- Vascular Tumors and Angiosarcomas
- Tuberous Sclerosis Complex Research
- AI in cancer detection
- Renal cell carcinoma treatment
- PI3K/AKT/mTOR signaling in cancer
- PARP inhibition in cancer therapy
- Cancer-related molecular mechanisms research
- DNA Repair Mechanisms
- Molecular Biology Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Hepatocellular Carcinoma Treatment and Prognosis
Johns Hopkins University
2023-2025
Johns Hopkins Medicine
2023-2025
University of Baltimore
2023
5003 Background: Docetaxel (Doce) is effective for metastatic (M1) PC but its effect varied. Combining Doce and hormone therapy can improve overall survival (OS) not appropriate all. We previously reported the mRNA Decipher test predicts benefit from Doce. then used transcriptome-wide data to interrogate differential associations with outcome biologically-relevant pathways. Methods: PTEN inactivation using a described signature (Liu et al JCI, 2021; active score <= 0.3, inactive: >...
Fatty acid synthase (FASN) catalyzes the synthesis of long-chain saturated fatty acids and is overexpressed during prostatic tumorigenesis, where it therapeutic target in several ongoing trials. However, mechanism FASN upregulation prostate cancer remains unclear. Here, we examine gene CpG methylation pattern by InfiniumEPIC profiling whole-genome bisulfite sequencing across multiple racially diverse primary metastatic cohorts, comparing with protein expression as measured digitally...
<div>Abstract<p>Fatty acid synthase (FASN) catalyzes the synthesis of long-chain saturated fatty acids and is overexpressed during prostatic tumorigenesis, where it therapeutic target in several ongoing trials. However, mechanism FASN upregulation prostate cancer remains unclear. Here, we examine <i>FASN</i> gene CpG methylation pattern by InfiniumEPIC profiling whole-genome bisulfite sequencing across multiple racially diverse primary metastatic cohorts, comparing...
<p>FASN gene expression is significantly associated with ERG status in NCI primary tumor cohort.</p>
<p>FASN protein expression in tumor glands is inversely correlated with <i>FASN</i> gene methylation tissue primary prostate tumors. <b>A,</b> Representative plots of beta values (range: 0–1) by probe on InfiniumEPIC for three tumors among the lowest (blue, H-score range from 3–4) and highest (red, 289–292) mean FASN JHU cohort. Mean are negatively levels immunostaining cohort (<b>B</b>) RPPA TCGA (<b>C</b>). Each point B C represents an...
<p>Representative whole genome bisulfite sequencing in the WCDT cohort for TMPRSS2 and KLK3.</p>
<p>Correlation between FASN gene expression and methylation in the (A) JHU cohort, (B) TCGA (C) NCI cohort.</p>
<p>FASN gene expression is upregulated and FASN hypomethylated in primary prostate tumors from the TCGA tumor cohort.</p>
<p>FASN gene expression is upregulated and FASN hypomethylated in primary prostate tumors from the NCI tumor cohort.</p>
<p>Correlation between FASN gene expression and methylation in the (A) JHU cohort, (B) TCGA (C) NCI cohort.</p>
<div>Abstract<p>Fatty acid synthase (FASN) catalyzes the synthesis of long-chain saturated fatty acids and is overexpressed during prostatic tumorigenesis, where it therapeutic target in several ongoing trials. However, mechanism FASN upregulation prostate cancer remains unclear. Here, we examine <i>FASN</i> gene CpG methylation pattern by InfiniumEPIC profiling whole-genome bisulfite sequencing across multiple racially diverse primary metastatic cohorts, comparing...
<p>FASN protein expression, and to a lesser extent <i>FASN</i> gene methylation, is associated with ERG status in primary tumor cohort. <b>A,</b> FASN expression by immunostaining higher ERG+ compared ERG− cases the JHU cohort; each point represents an individual tumor. <b>B,</b> also increased from PCBN <b>C,</b><i>FASN</i> probe methylation varies significantly for several probes. <b>D,</b><i>FASN</i>...
<p>FASN protein expression in tumor glands is inversely correlated with <i>FASN</i> gene methylation tissue metastatic CRPC cohort. <b>A,</b> Representative plots of CpG level by WGBS corresponding FASN mRNA (TPM) for three representative tumors low (blue) and high (red) the SU2C WCDT Mean negatively samples; each point represents an individual <b>B</b>.</p>
<p>FASN gene expression is upregulated and FASN hypomethylated in primary prostate tumors from the TCGA tumor cohort.</p>
<p>Digital quantification of FASN immunostaining in prostate tumor cells</p>
<p>FASN gene expression is significantly associated with ERG status in NCI primary tumor cohort.</p>
<p>FASN protein expression is increased in prostate tumors from germline <i>HOXB13</i> G84E carriers compared with matched WT controls. <b>A,</b> FASN by immunostaining higher than <b>B,</b> ERG status not associated among carriers, though seen ERG-positive ERG-negative controls (****, <i>P</i> < 0.0001).</p>