A5070799904- Nanoplatforms for cancer theranostics
- Advanced Nanomaterials in Catalysis
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Machine Learning in Materials Science
- Monoclonal and Polyclonal Antibodies Research
- Nanocluster Synthesis and Applications
- Advanced Photocatalysis Techniques
- Electrochemical sensors and biosensors
- Coronary Interventions and Diagnostics
- Boron Compounds in Chemistry
- Metal-Organic Frameworks: Synthesis and Applications
- Photoacoustic and Ultrasonic Imaging
- CAR-T cell therapy research
- Angiogenesis and VEGF in Cancer
- Gas Sensing Nanomaterials and Sensors
- Electrospun Nanofibers in Biomedical Applications
- Copper-based nanomaterials and applications
- Immunotherapy and Immune Responses
- Nanofabrication and Lithography Techniques
- Graphene and Nanomaterials Applications
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
Nankai University
2021-2025
State Key Laboratory of Medicinal Chemical Biology
2021-2024
Ministry of Education
2022
Yancheng Teachers University
2019-2021
Designing dual-targeted nanomedicines to enhance tumor delivery efficacy is a complex challenge, largely due the barrier posed by blood vessels during systemic delivery. Effective transport across endothelial cells is, therefore, critical topic of study. Herein, we present synthetic biology-based approach engineer ferritin nanocages (Dt-FTn) for understanding receptor-mediated cells. By leveraging genetically engineered logic-gated strategy, coassembled various Dt-FTn in E. coli with tunable...
Nanozymes play a pivotal role in mitigating excessive oxidative stress, however, determining their specific enzyme-mimicking activities for intracellular free radical scavenging is challenging due to endo-lysosomal entrapment. In this study, we employ genetic engineering strategy generate ionizable ferritin nanocages (iFTn), enabling escape from endo-lysosomes and entry into the cytoplasm. Specifically, repeated Histidine-Histidine-Glutamic acid (9H2E) sequences are genetically incorporated...
Biomimetic design of nanomaterials with enzyme-like characteristics has emerged as a promising method for the generation novel therapeutics. However, synthesis while maintaining high degree control over both geometry and valency poses prominent challenge. Herein, authors introduce nanomaterial-based synthetic biology strategy accurate quantitative tailoring high-ordered nanostructures that uses "bottom-up" hierarchical incorporation protein building blocks. The assembled nano-oligomers...
Abstract Despite the promise in whole‐tumor cell vaccines, a key challenge is to overcome lack of costimulatory signals. Here, agonistic‐antibody‐boosted tumor nanovaccines are reported by genetically engineered antibody‐anchored membrane (AAM) technology, capable effectively activating pathways. Specifically, AAM can be stably constructed following genetic engineering membranes with anti‐CD40 single chain variable fragment (scFv), an agonistic antibody induce The versatilely designed and...
Abstract Well‐defined nanostructures are crucial for precisely understanding nano‐bio interactions. However, nanoparticles (NPs) fabricated through conventional synthesis approaches often lack poor controllability and reproducibility. Herein, a synthetic biology‐based strategy is introduced to fabricate uniformly reproducible protein‐based NPs, achieving precise control over heterogeneous components of the NPs. Specifically, ferritin assembly toolbox system developed that enables...
Abstract The emergence of nanomedicine has provided a promising strategy to greatly enhance the therapeutic efficacy O 2 ‐dependent photodynamic therapy (PDT). However, plasma‐derived protein corona and/or discontinuous supply substantially limit their tumor delivery efficiency and outcomes. Herein, cloaking‐based cascade nanozymes are developed using genetically engineered human ferritin heavy chain nanocages (FTn) as unique pre‐coated steady suppliers. Specifically, FTn is coated onto...
Abstract T cell engagers, which bind tumor‐associated antigens and specific molecules, represent a promising class of immunotherapies for enhancing targeted immune responses. Here, “plug‐and‐display” platform is introduced engineering nanoengagers by anchoring antibody fragments into lipid‐based nanoparticles. This approach utilizes genetically engineered lipoprotein fused with single‐chain variable (scFv) nanobodies, spontaneously integrate lipid bilayer the nanoparticles, achieving high...
The development of mitochondria-targeting nanozymes holds significant promise for treating myocardial ischemia-reperfusion (IR) injury but faces biological barriers. To overcome these obstacles, we herein utilized genetically engineered ferritin nanocages (i.e., imFTn) to develop consisting three subunit assembly modules: an IR-injured cardiomyocyte-targeting module, a lysosome-escaping and module. Using imFTn as nanozyme platform, developed capable efficiently catalyzing the l-Arg substrate...
<title>Abstract</title> Nanozymes play a pivotal role in mitigating excessive oxidative stress, however, determining their specific enzyme-mimicking activities for intracellular free radical scavenging is challenging due to endo-lysosomal entrapment. In this study, we employed genetic engineering strategy generate ionizable ferritin nanocages (iFTn), enabling escape from endo-lysosomes and entry into the cytoplasm. Specifically, repeated Histidine-Histidine-Glutamic acid (9H2E) sequences...
Abstract The central dogma of nanoparticle delivery to tumours through enhanced leakiness vasculatures has become a topic debate in recent years. To address this problem, we created single-vessel quantitative analysis method by taking advantage protein-based nanoprobes and image segmentation-based machine learning technology (Nano-ISML). Using Nano-ISML, quantified > 50,000 individual blood vessels from 32 tumour models, which revealed highly heterogenous vascular permeability...