A5091133884- Cancer therapeutics and mechanisms
- Cancer Immunotherapy and Biomarkers
- Estrogen and related hormone effects
- HER2/EGFR in Cancer Research
- Advanced Breast Cancer Therapies
- Synthesis and biological activity
- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- Occupational and environmental lung diseases
- Monoclonal and Polyclonal Antibodies Research
- Cardiac Imaging and Diagnostics
- Metabolism, Diabetes, and Cancer
- Coronary Interventions and Diagnostics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pleural and Pulmonary Diseases
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Hematopoietic Stem Cell Transplantation
- Aluminum toxicity and tolerance in plants and animals
- CAR-T cell therapy research
- Acute Myocardial Infarction Research
- Tissue Engineering and Regenerative Medicine
- Protease and Inhibitor Mechanisms
- Lung Cancer Treatments and Mutations
- Protein Kinase Regulation and GTPase Signaling
Dana-Farber Cancer Institute
2024
Dana-Farber Brigham Cancer Center
2024
Brigham and Women's Hospital
2024
Duke University
2016-2020
Oxford University Press (United Kingdom)
2020
The University of Texas MD Anderson Cancer Center
2017-2018
Louis Stokes Cleveland VA Medical Center
2012-2017
Massachusetts General Hospital
2016
Clinical Research Institute
2016
University Hospitals Cleveland Medical Center
2012
Insulin receptor substrate (IRS) proteins are important intracellular molecules that mediate insulin tyrosine kinase signaling. A decreased content of IRS has been found in insulin-resistant states animals, humans, and cultured cells under various conditions. However, the molecular mechanism controls cellular levels is unknown. We report chronic treatment induces degradation IRS-1, but not IRS-2, protein cells. The insulin-induced IRS-1 can be prevented by pretreatment with lactacystin, a...
Stimulation of cell signaling cascades by oxidants may be important in the pathogenesis pulmonary and pleural diseases. Here, we demonstrate rat mesothelial cells that apoptotic concentrations crocidolite asbestos H 2 O induce phosphorylation activation extracellular signal-regulated protein kinases (ERK). Activation c- jun-NH -terminal (JNK)/stress-activated was also observed response to . In contrast, caused more protracted ERK without JNK activation. Both - asbestos-induced abolished...
Women with chronic coronary disease are generally older than men and have more comorbidities but less atherosclerosis. We explored sex differences in revascularization, guideline-directed medical therapy, outcomes among patients ischemia on stress testing, without invasive management.
BACKGROUND: The appropriate use criteria for revascularization of stable ischemic heart disease have not been evaluated using randomized data. Using data from the ISCHEMIA trial (International Study Comparative Health Effectiveness with Medical and Invasive Approaches; July 2012 to January 2018, 37 countries), health status benefits an invasive strategy over a conservative one were examined within scenarios. METHODS: Among 1833 participants mapped 36 scenarios, symptom was assessed Seattle...
Abstract Estrogen receptor–positive (ER+) breast cancer is not considered immunogenic and, to date, has been proven resistant immunotherapy. Endocrine therapy remains the cornerstone of treatment for ER+ cancers. However, constitutively activating mutations in estrogen receptor alpha (ESR1) gene can emerge during treatment, rendering tumors endocrine therapy. Although these represent a pathway resistance, they also potential source neoepitopes that be targeted by In this study, we...
We examined the mechanisms of interaction crocidolite asbestos fibers with epidermal growth factor (EGF) receptor (EGFR) and role EGFR-extracellular signal-regulated kinase (ERK) signaling pathway in early-response protooncogene (c- fos/c- jun) expression apoptosis induced by rat pleural mesothelial (RPM) cells. Asbestos fibers, but not nonfibrous analog riebeckite, abolished binding EGF to EGFR. This was due a direct ligand, inasmuch as studies using absence membranes showed that did adsorb...
We developed in situ dual-fluorescence detection techniques for measuring apoptosis and proliferation simultaneously single dishes of cells. The deoxyribonucleic acid (DNA)-specific labeling method, terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate nick-end (TUNEL), first was used conjunction with a 4',6-diamidino-2-phenylindole (DAPI) counterstain to detect measure morphologic characteristics apoptotic rat pleural mesothelial (RPM) cells isolated from Fischer...
In recent years, it has become apparent that minerals can trigger alterations in gene expression by initiating signaling events upstream of transactivation. These cascades may be initiated at the cell surface after interaction with plasma membrane either through receptorlike mechanisms or integrins. Alternatively, pathways stimulated active oxygen species generated both during phagocytosis and redox reactions on mineral surface. At least two linked to activation transcription factors, i.e.,...
Background: Clinical trials using intracoronary (IC) delivery of cells have addressed efficacy but the optimal technique is unknown. Our study aimed to determine whether transvenous intramyocardial (TVIM) approach was advantageous for cellular retention in AMI. Methods: Domestic pigs (n = 4) underwent catheterization with coronary angiography and ventriculography prior infarction pre‐ post‐cells. Pigs 90‐minute balloon occlusion left anterior descending artery (LAD). After one week they were...
Cathepsin G (CG) is a myeloid azurophil granule protease that highly expressed by acute leukemia (AML) blasts and stem cells. We previously identified CG1 (FLLPTGAEA), human leukocyte antigen-A2-restricted nonameric peptide derived from CG, as an immunogenic target in AML. In this report, we aimed to assess the level of CG expression lymphoid (ALL) its potential immunotherapeutic ALL. Using RT-PCR western blots, mRNA protein, respectively, B-ALL patient samples cell lines. also examined...
Objectives To evaluate potential gaps in preventive medical therapy and healthy lifestyle practices among symptomatic patients with suspected coronary artery disease (CAD) seeing primary care physicians cardiologists how vary by sociodemographic characteristics baseline cardiovascular risk. Design Cross-sectional study assessing gaps. Participants 10 003 outpatients evaluated physicians, or other specialists for CAD. Setting PROspective Multicenter Imaging Study Evaluation of Chest Painfrom...
<h3>Background</h3> T cells are generally present in low numbers estrogen receptor-positive (ER+) breast cancer (BC), potentially due to limited antigens or impaired antigen presentation. However, the defined mechanisms remain unclear. <h3>Methods</h3> This study investigates interplay between receptor signaling (ERS), presentation machinery (APM), and cell (TC) using diverse datasets, including transcriptome of 54 BC lines CCLE, hundreds patients from TCGA, METABRIC, ACOSOG Z1301B trial (an...
<div>Abstract<p>Estrogen receptor–positive (ER<sup>+</sup>) breast cancer is not considered immunogenic and, to date, has been proven resistant immunotherapy. Endocrine therapy remains the cornerstone of treatment for ER<sup>+</sup> cancers. However, constitutively activating mutations in estrogen receptor alpha (<i>ESR1</i>) gene can emerge during treatment, rendering tumors endocrine therapy. Although these represent a pathway resistance,...
<p>Peptide-specific expansion of CD8<sup>+</sup> T cells. PBMCs from healthy donors were isolated and expanded as described in the Materials Methods. Tetramer staining was performed pre- post-expansion to quantify frequencies peptide-specific <b>A,</b> Wildtype-1, E380Q. <b>B,</b> Wildtype-2, Y537S, D538G. Data represents three independent experiments, 3 separate donors, triplicate. Statistical significance determined via comparison pre-expansion...
<p>Peptide-specific CTLs effectively kill ER<sup>+</sup> breast tumor cells. MCF7 cells were pulsed with corresponding peptides and labeled calcein-AM. Cytotoxicity was determined by a standard calcein-AM release assay. peptides. CG1-pulsed non-pulsed used as negative controls. Statistical significance via one-way ANOVA. Data show percent cytotoxicity at the 10:1 effector:target ratio, represent average of five experiments (run in triplicate) from different healthy female...