A5077082871- Acute Myeloid Leukemia Research
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Chronic Myeloid Leukemia Treatments
- Sarcoma Diagnosis and Treatment
- Digital Imaging for Blood Diseases
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immune Cell Function and Interaction
- Advanced Proteomics Techniques and Applications
- Acute Lymphoblastic Leukemia research
- Advanced Computing and Algorithms
- Molecular Biology Techniques and Applications
- Bone and Joint Diseases
The University of Texas MD Anderson Cancer Center
2023-2025
University of Houston
2024-2025
Scripps MD Anderson Cancer Center
2024
Abstract Interferon gamma (IFNγ) is a critical cytokine known for its diverse roles in immune regulation, inflammation, and tumor surveillance. However, while IFNγ levels were elevated sera of most newly diagnosed acute myeloid leukemia (AML) patients, complex interplay AML remains insufficiently understood. We aim to characterize these interactions through comprehensive bulk single-cell approaches bone marrow patients. identify monocytic as having unique microenvironment characterized by...
Monosomy 7 and 7q deletions (-7/del(7q)) are the most common adverse cytogenetic event in acute myeloid leukemia (AML), linked to high relapse rates. We analyzed 115 AML patients with -7/del(7q) who achieved remission after induction therapy characterize mutational landscape from diagnosis relapse. Median overall survival (OS) was 10.4 months, improved without TP53 mutation (13.04 vs. 8.6 months) or complex karyotype (12.4 months). mutations were frequent (67% of cases at diagnosis)...
Acute myeloid leukemia (AML) resides in an immune-rich microenvironment, yet, immune-based therapies have faltered eliciting durable responses. Bridging this paradox requires a comprehensive understanding of leukemic interactions within the bone marrow microenvironment. We optimized high-throughput tissue-microarray-based pipeline for high-plex spatial immunofluorescence and mass cytometry imaging on single slide, capturing immune, tumor, structural components. Using unbiased clustering K...
6547 Background: Chromosomal 7 deletions (del7/7q) are the most common adverse cytogenetic event in acute myeloid leukemia and associated with high rate of relapse. We analyzed gene mutation profiles del7/7q AML patients who relapsed after initial remission to identify molecular patterns clinical outcomes. Methods: Out 351 newly diagnosed del7/7q, 115 (32%) achieved complete first-line treatment between 2012-2023. Of patients, 77 (67%) during study follow-up. Kaplan-Meier Cox regression were...
Abstract Acute myeloid leukemia (AML) resides in an immune rich microenvironment, yet, immune-based therapies have faltered eliciting durable responses. Bridging this paradox requires a comprehensive understanding of leukemic interactions within the bone marrow microenvironment. We optimized high-throughput tissue-microarray based pipeline for high-plex spatial immunofluorescence and mass cytometry imaging on single slide, capturing immune, tumor, structural components. Using unbiased...
Abstract Acute myeloid leukemia (AML) is a heterogeneous disease of the bone marrow (medullary) but can also involve extramedullary tissues. While single cell dynamics AML in suspension are previously explored, comprehensive spatial transcriptomic assessment remain underexplored. Here, we used Visium transcriptomics to resolve medullary and environments. We reveal co-localization monocytes granulocyte-monocyte progenitors with leukemic populations marrow, sharing molecular signatures sites....
Abstract Acute Myeloid Leukemia (AML) is a heterogenous disease characterized by immature blasts at different states of differentiation resulting in marked intra-tumor heterogeneity (ITH). Compared to patients with diploid cytogenetics, deletions chromosome 7/7q (del7/7q), 5/5q (del5/5q) or double deletion (del5/5q & del 7/7q) confer worse outcomes AML. We conducted single cell RNA (scRNA) profiling bone marrow mononulear cells (BMMCs) from 20 newly diagnosed adult AML (n=7), del5/5q...