A5008993921- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Molecular Sensors and Ion Detection
- Gut microbiota and health
- Cellular Mechanics and Interactions
- Optical Coherence Tomography Applications
- Probiotics and Fermented Foods
- Legume Nitrogen Fixing Symbiosis
- RNA Research and Splicing
- Nanoplatforms for cancer theranostics
- Robot Manipulation and Learning
- Antimicrobial Peptides and Activities
- Polysaccharides and Plant Cell Walls
- Microfluidic and Bio-sensing Technologies
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Near-Field Optical Microscopy
- Light effects on plants
- Molecular Biology Techniques and Applications
- Digestive system and related health
- Barrier Structure and Function Studies
- Robotic Path Planning Algorithms
- Radical Photochemical Reactions
- Clinical Nutrition and Gastroenterology
- Microbial Inactivation Methods
Chinese University of Hong Kong, Shenzhen
2024
Kunming University of Science and Technology
2021-2024
Yunnan Yingmao Biotechnology (China)
2023-2024
Shenzhen Bay Laboratory
2023-2024
Karamay Central Hospital of Xinjiang
2023
Suzhou Institute of Biomedical Engineering and Technology
2015-2021
Chinese Academy of Sciences
2018-2021
Center for Excellence in Molecular Cell Science
2020-2021
Tsinghua University
2015
Lanzhou University
2009-2014
Abstract Structured illumination microscopy (SIM) has become a widely used tool for insight into biomedical challenges due to its rapid, long-term, and super-resolution (SR) imaging. However, artifacts that often appear in SIM images have long brought question fidelity, might cause misinterpretation of biological structures. We present HiFi-SIM, high-fidelity reconstruction algorithm, by engineering the effective point spread function (PSF) an ideal form. HiFi-SIM can effectively reduce...
As a kind of potential probiotic, Akkermansia muciniphila abundance in human body is directly causally related to obesity, diabetes, inflammation and abnormal metabolism. In this study, A. dynamic cultures using five different media were implemented an vitro bionic intestinal reactor for the first time instead traditional static culture brain heart infusion broth (BHI) or BHI + porcine mucin (BPM).The biomass under BPM reached 1.92 g/L, which improved 44.36% compared with value BPM. The (HM)...
Telekinesis, as commonly portrayed in science fiction literature and cinema, is a super power wherein users control manipulate objects absent physical interaction. In real world, enhancing human-robot interaction needs the synthesis of human intuitive processes with robotic arm. This paper introduces teleoperation system achieving essence telekinetic operations, combining profound capabilities augmented reality (AR) arm operations. Utilizing AR, proposed methodology offers operators visual...
The question of how cells sense substrate mechanical cues has gained increasing attention among biologists. By introducing contour-based data analysis to single-cell force spectroscopy, we identified a loading-rate threshold for the integrin α2β1–DGEA bond beyond which dramatic increase in lifetime was observed. On basis (elasticity or topography), effective spring constant substrates k is mapped loading rate r under actomyosin pulling speed v, which, turn, affects integrin–ligand bond....
Abstract Mental rotation, one of the cores spatial cognitive abilities, is closely associated with processing and general intelligence. Although brain underpinnings mental rotation have been reported, cellular molecular mechanisms remain unexplored. Here, we used magnetic resonance imaging, a whole-brain distribution atlas 19 neurotransmitter receptors, transcriptomic data from Allen Human Brain Atlas, performances 356 healthy individuals to identify genetic/molecular foundation rotation. We...
Capillary imbibition plays an essential role in the flow behavior of unconventional reservoirs. The severe heterogeneity pore structures formations can lead to different processes and dynamics compared conventional This study investigates process heterogeneous networks by first examining between pores using ideal capillary model with interacting microchannel micromodels. results reveal that water preferentially imbibes into small microchannels rather than large ones, velocity decreases width...
Opal shale, as a naturally occurring and noncrystalline silica material with porous structure, has the potential to be drug delivery carrier. In this study, we obtained opal shale nanoparticles (OS NPs) through techniques of ultrasonic emulsion differential centrifugation. The OS NPs exhibited markedly lower cytotoxicity than crystalline mesoporous nanoparticles. highly structure strong adsorbability endowed ability loading sustained release doxorubicin (DOX). DOX-loaded improved tumor...
Mechanical stimuli play crucial roles in bone remodeling and resorption. Osteopontin (OPN), a marker for osteoblasts, is important cell communication matrix mineralization, known to function during mechanotransduction. Hypergravity convenient approach forge mechanical on cells. It has positive effects certain markers of osteoblast maturation, making it possible strategy tissue engineering. We investigated the hypergravity OPN expression signaling osteoblasts. treatment at 20 g 24 hours...
Direct <italic>N</italic>-nitrosation of <italic>N</italic>,<italic>N</italic>-dialkylanilines under mild, non-acidic and non-oxidative conditions.
Phenotype analysis of yeast cell requires high-throughput imaging and automatic abundant image data. At first, each needs to be segmented labeled in the bright-field images. However, ambiguous boundary images leads failure traditional segmentation algorithms. We propose a algorithm based on morphological characteristics cells. Seed points are first identified along contour then connected by an edge tracing approach. In this way, “ill-detected” noise removed so that edges cells can...
Stem cell therapy has shown great clinical potential in oncology, injury, inflammation, and cardiovascular disease. However, due to the technical limitations of vivo visualization transplanted stem cells, therapeutic mechanisms biosafety cells are poorly defined, which limits speed translation. The commonly used methods for tracing currently include optical imaging, magnetic resonance imaging (MRI), nuclear medicine imaging. involves radioactive materials, MRI low resolution at cellular...
Low temperature significantly influences chloroplast development and chlorophyll (Chl) biosynthesis, so effect of coldness on Chl content fluorescence characteristics was investigated in C. bungeana (Chorispora Fisch. & C.A. Mey). The levels transcript protein an enzymatic step during biosynthesis response to chilling (4°C) freezing (-4°C) were also examined this work. Significant reduction total observed, but the much less at 4°C than that -4°C. Moreover, maximal quantum efficiency...
Structured illumination microscopy (SIM) is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly used fluorescent labeling methods. can be obtained by either laser interference or projection of fringe patterns. Here, we proposed projector composed compact multi-wavelength LEDs module digital micromirror device (DMD) which directly attached commercial inverted microscopes update it into SIM system. The effects the...
The pH-responsive profile of the traditional pH probes (the HH-type probes) is governed by Henderson–Hasselbalch equation. Despite their widespread use, with further enhanced sensitivity, i.e., Hill-type probes, are sought after. As a result positive cooperativity toward protonation, they exhibit smaller acid–base transition width than that probe, which 2 unit. result, have an improved capability to differentiate minor biorelevant changes. We previously devised class small-molecule (PHX) pKa...
Expansion microscopy has enabled super resolution imaging of biological samples. The accurate measurement expansion factor and distortion typically requires locating the same region interest in sample before after expansion, which is often time-consuming to achieve. Here we introduce a convenient method for relocation by utilizing isolated porcine glomeruli as landmarks during expansion. Following heat denaturation proteinase K digestion protocols, exhibit 3.5 4 (only 7%-16% less expanded...