Aims: Opening of the mitochondrial permeability transition pore (mPTP) is a critical event during ischemia/reperfusion (I/R) injury. Recently, we showed that Panax quinquefolium saponin (PQS) alleviates apoptosis cardiomyocytes by suppressing excessive endoplasmic reticulum stress (ERS) I/R Here, hypothesized this anti-apoptotic effect might be mediated through inhibition mPTP and apoptotic pathway. Methods: Ninety-six healthy male Sprague-Dawley rats were randomly divided into sham, I/R,...

Traditional Chinese medicine is one of the complementary and alternative therapies to improve prognosis coronary heart disease (CHD). Taohong Siwu Decoction (THSWD), a classical traditional medication that promotes blood circulation, clinically beneficial in CHD. However, underlying mechanism THSWD still unclear. To comprehensively understand material foundation 'blood', it significantly important study differential metabolites involved treatment CHD with medicinal herb promoting circulation...

Cardiac rehabilitation (CR) is a multicomponent intervention to reduce adverse outcomes from coronary artery disease, but its mechanisms are not fully understood. The aims of this study were examine the impact CR on survival and cardiovascular risk factors, determine potential mediators between attendance reduced mortality.

Coronary heart disease (CHD) remains the leading cause of morbidity and mortality worldwide. Traditional Chinese medicine (TCM) is one effective complementary alternative therapies used to improve prognosis CHD patients. Xuefu Zhuyu Decoction, a classical traditional medication for regulating Qi promoting blood circulation, has clinical benefit in CHD; however, underlying mechanism not clear. Recently, it was found that metabolites involved amino acid metabolism tricarboxylic cycle were...

Zedoarondiol, a sesquiterpene lactone compound, showed an anti-proliferative activity on vascular smooth muscle cells in our previous study. However, whether it has beneficial effect endothelial injury induced by oxidized low-density lipoprotein (ox-LDL) remains unclear. This study was designed to investigate the protective of zedoarondiol ox-LDL-induced and explored its underlying mechanism.The human umbilical vein (HUVECs) were evaluated Cell Counting Kit-8 (CCK-8) assay released lactic...

<title>Abstract</title> Background Propionic acidemia (PA) is a rare autosomal recessive metabolic disorder caused by defects in propionyl-CoA carboxylase (PCC), mitochondrial enzyme composed of six alpha (PCCA) and beta (PCCB) subunits. Mutations <italic>PCCA/PCCB</italic> genes disrupt PCC function, leading to toxic metabolite accumulation clinical manifestations. Current research limited inadequate patient-derived cellular models ethical constraints sample acquisition. Method Using...

Impaired autophagic flux contributes to cardiomyocyte death in ischemia/reperfusion (I/R) injury. Restoring the impaired by using agents may be a promising strategy that alleviates myocardial I/R The present study aimed evaluate effect of exogenous calreticulin (CRT) postconditioning on induced hypoxia/reoxygenation (H/R) injury H9c2 cells.Rat model was prepared. CRT postconditionging fulfilled an intraperitoneal injection (0.5 mg/kg body weight) 5 min before reperfusion. Hemodynamics, serum...

With respect to peak carbon and neutrality, China’s economic structure is developing towards low carbonization, circulation, cleanliness. There an urgent need expand the scope of cleaner production audits, improve efficiency, promote through regional coordination. The 14th Five-Year National Cleaner Production Implementation Plan proposed selecting 100 parks or industrial clusters conduct overall audit innovation pilot. To coordinated development areas, this study constructed a set index...

Postconditioning prevents cardiomyocytes from ischemia/reperfusion-induced apoptosis. However, little is known about the molecular mechanisms that mediate cardioprotective effect of postconditioning. Here, we hypothesized postconditioning targeted p53 upregulated modulator apoptosis (PUMA) to protect against endoplasmic reticulum stress-mediated Our results demonstrated could inhibit GRP78 (78-kDa glucose-regulated protein) expression, caspase-12 activation, and cardiomyocyte by regulating...

Endoplasmic reticulum (ER) stress-related apoptosis is involved in the pathophysiology of many cardiovascular diseases, and Panax quinquefolium saponin (PQS) able to inhibit excessive ER cardiomyocytes following hypoxia/reoxygenation myocardial infarction. However, pathway by which PQS inhibits not well understood. To further investigate protective effect against apoptosis, primary cultured were stimulated with thapsigargin (TG), widely used model cellular stress, it could induce apoptotic...

Background/Aims: Vascular smooth muscle cells (VSMCs) proliferation contributes significantly to atherosclerosis and in-stent restenosis. Platelet-derived growth factor-BB (PDGF-BB) plays a vital role in VSMCs proliferation. Zedoarondiol, sesquiterpene lactone compound, has an anti-inflammatory activity. However, the of zedoarondiol PDGF-BB-mediated remains unclear. In this study, we investigated effects on PDGF-BB-induced explored possible mechanisms. Methods: The inhibitory were evaluated...

Despite advances in the drug treatment strategy for stable coronary heart disease (CHD), mortality of CHD continues to rise. New or adjuvant treatments would be desirable CHD. Xuefu Zhuyu granules are derived from formula traditional Chinese medicine. To determine whether might have effects on CHD, we conducted a controlled clinical trial. Patients with were enrolled and randomly assigned receive placebo 12 weeks addition their standard medications The primary endpoints comprise Canadian...

Depressive symptoms have been found to be highly prevalent among patients with coronary heart disease (CHD) and seriously affect the patients' quality of life. However, most psychotropic drugs warnings about potential side effects. Accordingly, safer effective alternatives are urgently demanded. Angina pectoris CHD is considered as "chest stuffiness heartache syndrome" in traditional Chinese medicine, major syndrome type named Qi stagnation blood stasis. Qi-regulating circulation-promoting...

To investigate whether myofibrillogenesis regulator 1 (MR-1) attenuates renal ischemia/reperfusion (I/R) injury via inhibiting phosphorylated Akt (p-Akt) mitochondrial translocation-mediated opening of the permeability transition pore (mPTP), we injected adenovirus containing MR-1 gene or its siRNAs to left kidney subcapsular areas Sprague-Dawley rats, which subsequently underwent experimental I/R injury. Renal functions and severity tubular were evaluated by serum creatinine blood urea...

Psychological stress can provoke and aggravate myocardial ischemia, this even trigger acute coronary syndromes or sudden cardiac death. Therefore, for the first time, study aimed to investigate method establishing a rat model of ischemia with psychological its evaluation.

Posttraumatic stress disorder (PTSD) is an independent risk factor for cardiovascular diseases. This study investigated the molecular mechanisms underlying myocardial injury induced by simulated PTSD.

Recent work reveals that actin acetylation modification has been linked to different normal and disease processes the effects associated with metabolic environmental stressors. Herein, we highlight of calreticulin on cell injury induced by microwave radiation in human microvascular endothelial (HMEC). HMEC was high-power power density (10, 30, 60, 100 mW/cm

Autophagy has been found to be involved in myocardial ischemia/reperfusion injury. However, the underlying mechanism and significance of autophagy reperfusion injury remain unclear. Herein, we evaluated effects exogenous calreticulin (CRT) on hypoxia/reoxygenation (H/R)-treated human microvascular endothelial cells (MECs).Human MECs were pretreated with CRT (25 pg/mL) for 30 min, followed by exposure an incubator filled a gas mixture 90% N2, 5% O2, CO2 8-h hypoxia. The then placed back...