Microcystins, the lethal cyanotoxins from Microcystis aeruginosa, can inhibit activity of protein phosphatase and promote liver tumors. Herein, a dual-modal split-type immunosensor was constructed to detect microcystin-LR (MC-LR), based on photocurrent change CdS/ZnO hollow nanorod arrays (HNRs) blue shift surface plasmon resonance peak Au nanobipyramids@Ag. By using mesoporous silica nanospheres as carrier immobilize secondary antibody DNA primer, hybridization chain reaction adopted...

Abstract Neutralization of bacterial toxins has become a compelling approach to treating infections as it may pose less selective pressure for the development resistance. Currently, majority toxin neutralization platforms act by targeting molecular structure toxin, which requires identification and customized design different diseases. Therefore, their been challenged enormous number complexity toxins. Herein, biomimetic nanosponges are formulated coating membranes human red blood cells...

Abstract Polymeric nanoparticles coated with membrane of intact red blood cells have emerged as biomimetic toxin nanosponges (RBC‐NS) that absorb and neutralize bacterial virulence factors associated numerous infections. Despite its promise, a clear correlation between in vitro neutralization complex toxins vivo therapeutic efficacy remains elusive. In this study, the whole secreted proteins (wSP) methicillin‐resistant Staphylococcus aureus (MRSA) are collected to induce lethality mice. The...

Colloidal gels consisting of oppositely charged nanoparticles are increasingly utilized for drug delivery and tissue engineering. Meanwhile, cell membrane-coated becoming a compelling biomimetic system innovative therapeutics. Here, we demonstrate the successful use as building blocks to formulate colloidal gel that gelates entirely based on material self-assembly without chemical cross-linking. Specifically, prepare red blood nanosponges mix them with an appropriate amount cationic...

Toxoplasma gondii is a unicellular protozoan parasite of the phylum Apicomplexa. The repeatedly goes through cycle invasion, division and induction host cell rupture, which an obligatory process for proliferation inside warm-blooded animals. It known that biology controlled by variety mechanisms ranging from genomic to epigenetic transcriptional regulation. In this study, we investigated global protein posttranslational lysine crotonylation 2-hydroxyisobutyrylation two T. strains, RH ME49,...

Mosquitoes transmit pathogens that cause infectious diseases of global importance. Techniques to easily introduce genes into mosquitoes, however, limit investigations the interaction between microbes and their arthropod vectors. We now show a cationic liposome significantly enhances delivery expression plasmid DNA in Aedes aegypti Anopheles gambiae mosquitoes. then introduced for Ae. thioester-containing proteins (AeTEPs), which are involved control flaviviral infection, mosquitoes using...

Malaria, a mosquito-borne disease caused by Plasmodium species, causes substantial morbidity and mortality throughout the world. sporozoites mature in oocysts formed mosquito gut wall then invade salivary glands, where they remain until transmitted to vertebrate host during bite. The circumsporozoite protein (CSP) binds glands plays role invasion of this organ sporozoites. We identified an Anopheles gland protein, named CSP-binding (CSPBP), that interacts with CSP. Downregulation CSPBP...

Abstract Aims The purpose of this study was to investigate the effects Bacillus subtilis supplementation on health weaned piglets and whether can reduce damage induced by ETEC K88. Methods Results experiment designed with a 2 × factorial arrangement, comprising control (CON) group, (PRO) Escherichia coli K88 (ETEC) + (PRO ETEC) group. Regardless presence ETEC, addition PRO increased piglets' final body weight (FW), average daily gain (ADG), feed intake (ADFI). Additionally, primarily...

Vaccination remains the most effective strategy for preventing infectious diseases. Subunit vaccines, which consist of antigenic components derived from pathogens, offer significant advantages in terms biosafety, ease preparation, and scalability. However, subunit vaccines often exhibit lower immunogenicity than whole-pathogen do. To address this limitation, coupling antigens with nanoparticles has emerged as a promising enhancing immune responses by mimicking pathogen structures improving...

Abstract Introduction: Fibroblast growth factor receptor (FGFR) alterations, including point mutations and gene fusions, play a pivotal role in various cancers, particularly urothelial carcinoma. Erdafitinib, an FDA-approved therapy for locally advanced or metastatic carcinoma with FGFR2/3 currently relies on tissue-based diagnostics, which are invasive may not always be feasible. Non-invasive liquid biopsy approaches, such as qPCR assays using urine cell pellets (UCP), offer significant...

Food safety issues caused by pesticide residue have exerted far-reaching impacts on human daily life, yet the available detection methods normally focus surface rather than penetration to internal area of foods. Herein, we demonstrated gold nanoparticle (AuNP)-immersed paper imprinting mass spectrometry imaging (MSI) for monitoring migration behaviors in various fruits and vegetables (i.e., apple, cucumber, pepper, plum, carrot, strawberry). By manually stamping food tissues onto...

Phenotype D-- is associated with severe hemolytic transfusion reactions and disease of the fetus newborn. It typically caused by defective RHCE genes. In this study, we identified a phenotype proband verified Rh phenotypes other 6 family members. However, inconsistent results between phenotypic analysis Sanger sequencing revealed intact exons no mutations in proband, but protein was not expressed. Subsequent whole-genome Oxford Nanopore Technologies an inversion ambiguous breakpoints intron...

Streptococcus suis is an important swine pathogen that can cause a variety of diseases. serotype 9 (SS9) prevalent serotype, but limited information available. Here, we studied and compared 30 SS9 isolates, including 24 isolates from China between year 2004 2013, 5 Vietnam reference isolate Denmark. A multilocus sequence typing (MLST) analysis was performed to exploit the genetic relationships those isolates. The phylogenetic tree based on MLST data divides into two clades (I II), revealing...

To investigate the presence and location of erm(T) in clinical Streptococcus suis isolates explore transmission ability fitness cost erm(T)-carrying mobile genetic elements among S. isolates, MICs were determined by broth microdilution. The was detected PCR. environment explored whole-genome sequencing (WGS) analysis. Intraspecies interspecies examined electrotransformation. associated with carriage an erm(T)-harboring plasmid or integrative conjugative element (ICE) competition experiments....

Cyclic di-guanosine monophosphate (c-di-GMP) is a unique bacterial second messenger but hijacked by host cells during infection as pathogen-associated molecular pattern (PAMP) to trigger STING-dependent immune responses. Here, we show that upon infection, VopY, an effector of Vibrio parahaemolyticus, injected into type III secretion system 2 (T3SS2), its pathogenic strains and indispensable for enterotoxicity. VopY EAL-domain-containing phosphodiesterase capable hydrolyzing c-di-GMP....