A5035803842- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Hematopoietic Stem Cell Transplantation
- Acute Lymphoblastic Leukemia research
- Glioma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Neuroblastoma Research and Treatments
- RNA Interference and Gene Delivery
- Childhood Cancer Survivors' Quality of Life
- Cancer therapeutics and mechanisms
- Cytomegalovirus and herpesvirus research
- Urban and spatial planning
- Financial Crisis of the 21st Century
- Immunodeficiency and Autoimmune Disorders
- Rural development and sustainability
- Herpesvirus Infections and Treatments
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Chronic Myeloid Leukemia Treatments
- Gastrointestinal disorders and treatments
- Chromatin Remodeling and Cancer
- Cardiac and Coronary Surgery Techniques
- Neurological Complications and Syndromes
- Ocular Oncology and Treatments
- Adipose Tissue and Metabolism
Osaka City General Hospital
2016-2025
Hirosaki University
2011-2023
Aichi Medical University
2013-2022
Ome Municipal General Hospital
2020
Doshisha Women's College of Liberal Arts
2018
Aichi Medical University Hospital
2016
National Sagamihara Hospital
2010-2013
Iwate University
2000-2011
Seiwa Hospital
2010
St. Jude Children's Research Hospital
2006-2010
Infusions of natural killer (NK) cells are an emerging tool for cancer immunotherapy. The development clinically applicable methods to produce large numbers fully functional NK is a critical step maximize the potential this approach. We determined capacity leukemia cell line K562 modified express membrane-bound form interleukin (IL)-15 and 41BB ligand (K562-mb15-41BBL) generate human with enhanced cytotoxicity. Seven-day coculture irradiated K562-mb15-41BBL induced median 21.6-fold expansion...
The present study was aimed to test the role of endothelin-1 (ET-1) as a possible autocrine/paracrine growth factor for cardiac fibroblasts, and examine its interaction with natriuretic hormones. Expression preproET-1 (ppET-1) mRNA by cultured fibroblasts from neonatal rats demonstrated Northern blot analysis using cDNA rat ppET-1 probe. Angiotensin II (ANG II) ET-1 transiently (30 min) increased steady-state levels in fibroblasts. Both ANG significantly stimulated [3H] thymidine...
BACKGROUND We have recently shown that angiotensin II-induced hypertrophy of cultured rat cardiomyocytes was partially blocked by an endothelin (ET) receptor antagonist (BQ123) selective for the ETA subtype, suggesting possible involvement endogenous ET-1 in mechanism cardiac vitro. In present study, we studied vivo blockade effects BQ123 on provoked left ventricular overload with aortic banding adult rats. METHODS AND RESULTS Forty rats were divided into four groups: (1) sham-operated...
To develop new therapies for children with solid tumors, we tested the cytotoxicity of natural killer (NK) cells expanded by coculture K562-mb15-41BBL cells. We sought to identify most sensitive tumor subtypes, clarify molecular interactions regulating cytotoxicity, and determine NK antitumor potential in vivo.We vitro against cell lines representative Ewing sarcoma (EWS; n = 5), rhabdomyosarcoma (n 4), neuroblastoma 3), osteosarcoma correlated results expression inhibitory activating...
A girl with congenital agammaglobulinemia and minor facial anomalies lacked B cells in peripheral blood: karyotypic analysis of white blood showed balanced translocation, t(9;20)(q33.2;q12). In the current study, we isolated a novel gene, leucine-rich repeat–containing 8 (LRRC8), at translocation site on chromosome 9. It has four transmembrane helixes one eight sequentially located repeats (LRRs) constitutes new protein family. is expressed T as well B-lineage cells. Translocation truncates...
The replicative potential of human CD56(+) CD3(-) natural killer (NK) cells is unknown. We found that by exposing NK to the leukaemic cell line K562 genetically modified express 4-1BB ligand and interleukin 15 (K562-mb15-41BBL), they expanded for up 30 population doublings, achieving numbers ranged from 1.6 x 10(5) 1.2 10(11)% (median, 5.9 10(6)%; n = 7) those originally seeded. However, eventually became unresponsive stimulation died. Their demise could be suppressed enforcing expression...
Retinoids are vitamin A derivatives and include trans-retinoic acid, isotretinoin, tamibarotene, bexarotene, all of which currently available for clinical use. The development retinoid therapy neuroblastoma has a history spanning more than four decades. most promising agent is can contribute to improving event-free survival in patients with high-risk by approximately 10% when administered over six months as maintenance therapy. Although isotretinoin regarded an essential component the...
Abstract The mechanisms responsible for myocardial injury and cell death in myocarditis are still unclear. We examined whether occurs via apoptosis myosin-induced autoimmune rats the Fas/Fas ligand (FasL) system plays a role this apoptosis. On days 14, 17, 21, 35 after immunization with porcine heart myosin, some cardiomyocytes infiltrating lymphocytes were found to be apoptotic on situ terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay, but none was...
Twenty-nine breast milk mononuclear cell samples were analyzed for human herpesvirus 7 (HHV-7) DNA, 6 (HHV-6) and cytomegalovirus (HCMV) DNA by polymerase chain reaction (PCR). In addition, peripheral blood from 13 puerperants HHV-7 PCR, seropositivity of was also in breast-fed bottle-fed children. detected 3 29 samples. HCMV samples, but HHV-6 not detected. 11 cells. Though the rate children slightly higher than that at 18 24 months old, difference statistically significant. From these...
In a previous study, we isolated novel gene, LRRC8 (leucine-rich repeat-containing 8), in girl with congenital agammaglobulinemia. We have now identified four unknown LRRC8-like genes, named TA-LRRP, AD158, LRRC5, and FLJ23420. Their predicted structures are very similar to each other, highly conserved between humans the mouse. All five genes encode proteins consisting of 16 extracellular leucine-rich repeats (LRRs), all which transmembrane regions except for These belong family, designated...
T-cell acute lymphocytic leukemia (T-ALL) with SPI1 fusion, a subtype first identified in Japan, has very poor prognosis. A 7-year-old boy was admitted to our hospital fever, cervical lymphadenopathy, eyelid edema, and purpura. White blood cell count markedly increased (551,000/µl). Flow cytometric analysis revealed cyCD3, CD1a, CD8, HLA-DR positive T-ALL, fusion gene screening TCF7::SPI1 fusion. The patient treated according the JPLSG ALL-T11 protocol. He responded poorly prednisolone, but...
Studies have suggested that bevacizumab has shown activity against various pediatric solid tumors. We, therefore, conducted a Phase I study of plus irinotecan in Japanese children with recurrent, progressive or refractory tumors.The starting dose was 10 mg/kg over 60-90 min and 125 mg/m(2) 90 intravenously on Days 1, 15 29. The 340 for patients receiving enzyme-inducing antiepileptic drugs. Treatment repeated every 6 weeks up to three courses the absence disease progression unacceptable...