A5079672398- Viral gastroenteritis research and epidemiology
- Infant Nutrition and Health
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Respiratory viral infections research
- Clinical Nutrition and Gastroenterology
- GDF15 and Related Biomarkers
- Nutrition and Health in Aging
- Congenital Anomalies and Fetal Surgery
- Macrophage Migration Inhibitory Factor
- Pneumocystis jirovecii pneumonia detection and treatment
- Organ Transplantation Techniques and Outcomes
- Pharmaceutical studies and practices
- Animal Behavior and Welfare Studies
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Neonatal Respiratory Health Research
- Pregnancy and Medication Impact
- Neonatal Health and Biochemistry
- Transplantation: Methods and Outcomes
- Escherichia coli research studies
- Metabolism and Genetic Disorders
- Probiotics and Fermented Foods
University of Copenhagen
2020-2025
Fecal filtrate transfer (FFT) is emerging as a safer alternative to traditional fecal microbiota transplantation (FMT) – particularly in the context of necrotizing enterocolitis (NEC), severe gastrointestinal condition affecting preterm infants. Using piglet model, FFT has demonstrated superiority over FMT safety and NEC prevention. Since virtually devoid bacteria, prokaryotic viruses (bacteriophages) are assumed mediate beneficial effects. However, this assumption remains unproven. To...
Growth differentiation factor 15 (GDF15) is a stress-induced cytokine. Although the exact physiological function of GDF15 not yet fully comprehended, significant elevation circulating levels during gestation suggests potential role for this hormone in pregnancy. This corroborated by genetic association studies which and receptor, GDNF family receptor alpha like (GFRAL) have been linked to morning sickness hyperemesis gravidarum (HG) humans. Here, we studied biology pregnancy mice, rats,...
Preterm infants have a high risk of intestinal inflammation which can progress to necrotizing enterocolitis (NEC). The gut microbial colonization commencing at birth is essential for proper maturation, but this process often disrupted in preterm infants, leading dysbiosis and increased developing NEC. Bacteriophages (phages), viruses that specifically infect bacteria, are an important constituent the microbiome protects epithelium against invading bacteria. This study aimed isolate...
Necrotizing enterocolitis (NEC) remains a frequent catastrophic disease in preterm infants, and fecal filtrate transfer (FFT) has emerged as promising prophylactic therapy. This study explored the role of virome viability for protective effect FFT. Using ultraviolet (UV) irradiation, we established viral inactivation protocol administered FFT, UV-inactivated FFT (iFFT) or sterile saline orally to piglets at risk experimental NEC. The gut pathology barrier properties were assessed, while...
Abstract Fecal virome transfer (FVT) shows promise in reducing necrotizing enterocolitis (NEC), likely due to donor bacteriophages preventing the gut dysbiosis preceding disease. However, concurrent of eukaryotic viruses may carry a risk infection for recipient. To increase safety, we investigated chemostat propagation as method eliminate from feces while maintaining diverse and reproducible bacteriophage community. Donor was collected healthy suckling piglets inoculated into fermenter...
Abstract Fecal virome transfer (FVT) shows promise in reducing necrotizing enterocolitis (NEC), likely due to donor bacteriophages preventing the gut dysbiosis preceding disease. However, concurrent of eukaryotic viruses may carry a risk infection for recipient. To increase safety, we investigated chemostat propagation as method eliminate from feces while maintaining diverse and reproducible bacteriophage community. Donor was collected healthy suckling piglets inoculated into fermenter...
Studies into the viral fraction of complex microbial communities, like in mammalian gut, have recently garnered much interest. Yet there is still no standardized protocol for extracting viruses from such samples, and protocols that exist employ procedures skew community sample one way or another. The first step extraction pipeline often consists basic filtering macromolecules bacteria, yet even this affects a strain-specific manner. In study, we investigate based on ultrafiltration how...
Abstract Background Necrotizing enterocolitis (NEC) is a serious condition, primarily affecting premature infants, in which portion of the gut undergoes inflammation and necrosis. Symptoms NEC are unspecific, together with rapid progression, disease remains significant concern. The preterm pig develops spontaneously, making it suitable model for exploring novel treatments. During piglet necropsy, NEC-lesions closely resemble pathologies found during surgery or autopsy infants. As such,...
Energy metabolism and immune response are tightly connected, but it is poorly understood how this interplay regulated in early life to dictate host defense strategy, infection risks severity. This particularly relevant for preterm, low birthweight or otherwise immunocompromised infants, who have poor metabolic control increased of sepsis. Here, we utilized data from the COPSAC2010 cohort with 700 mother-child pairs showed that plasma levels TCA cycle metabolites were associated reduced...
Preterm infants are at risk of multiple morbidities including necrotizing enterocolitis (NEC). Suspected NEC patients receive intravenous antibiotics (AB) to prevent sepsis, although enteral AB is arguably more effective reducing but rarely used due the resistance. Fecal microbiota transplantation (FMT) has shown protective effects against in animal experiments, interaction between and FMT not been investigated neonates. We hypothesized that administration followed by rectal would...
Abstract Background : Fecal filtrate transfer (FFT, i.e. “sterile filtered” fecal matter) is gaining increasing attention as a safer alternative to traditional microbiota transplantation (FMT) for treating gastrointestinal (GI) complications. Indeed, in the case of necrotizing enterocolitis (NEC), life-threatening GI emergency occurring preterm infants, FFT superior FMT terms both safety and efficacy when investigated piglets. Since virtually devoid bacteria, prokaryotic viruses...
Necrotizing enterocolitis (NEC) is a serious condition in premature infants, which portion of the intestine undergoes inflammation and necrosis. The preterm pig develops NEC spontaneously, making it suitable model for exploring novel treatments. We aimed to revise intestinal scoring system more accurately describe diversity lesions piglet model. included 333 piglets from four experiments, each delivered via cesarean section. were fed either gently processed (GP) or harshly (HP) milk formula...
Diarrhea is a major cause of reduced growth and mortality in piglets during the suckling weaning periods poses threat to global pig industry. gut dysbiosis may part be prevented via improved early postnatal microbial colonization gut. To secure better colonization, we hypothesized that transplantation colonic or gastric content from healthy donors newborn recipients would prevent diarrhea post-weaning period. Our objective was examine impact transplanting on health parameters paraclinical...
Abstract Necrotizing enterocolitis (NEC) remains a frequent catastrophic disease in preterm infants, but fecal filtrate transfer (FFT) has been identified as promising prophylactic therapy preclinical studies. This study examined the importance of FFT virome viability on gut colonization and NEC occurrence. We established an ultraviolet irradiation-based viral inactivation protocol demonstrated total loss infectivity mock community. Using this protocol, we inactivated aliquot...
Fecal virome transfer (FVT) shows promise in reducing necrotizing enterocolitis (NEC), likely due to donor bacteriophages preventing the gut dysbiosis preceding disease. However, concurrent of eukaryotic viruses may carry a risk infection for recipient. To increase safety, we investigated chemostat propagation as method eliminate from feces while maintaining diverse and reproducible bacteriophage community. Donor was collected healthy suckling piglets inoculated into fermenter containing...
Abstract GDF15 (growth differentiation factor 15) is a stress-induced cytokine. Although the exact physiological function of not yet fully comprehended, significant elevation circulating levels during gestation suggests potential role for this hormone in pregnancy. This corroborated by genetic association studies which and receptor, GDNF Family Receptor Alpha Like (GFRAL) have been linked to morning sickness hyperemesis gravidarum (HG) humans. Here, we studied biology pregnancy mice, rats,...
Abstract Background Diarrhea is a major cause of reduced growth and mortality in piglets during the suckling weaning periods poses threat to global pig industry. gut dysbiosis may part be prevented via improved early postnatal microbial colonization gut. To secure better colonization, we hypothesized that transplantation colonic or gastric content from healthy donors newborn recipients would prevent diarrhea until post-weaning stage. Our objective was examine impact transplanting on health...
Abstract Studies into the viral fraction of complex microbial communities like in mammalian gut have recently garnered much interest. Yet there is still no standardized protocol for extracting viruses from such samples, and protocols that exist employ procedures skew community sample one way or another. The first step extraction pipeline often consists basic filtering macromolecules bacteria, yet even this affects a strain-specific manner. In study we investigate based on ultrafiltration...