A5066295137- Infant Nutrition and Health
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Neonatal and Maternal Infections
- Neonatal Respiratory Health Research
- Clinical Nutrition and Gastroenterology
- Neonatal and fetal brain pathology
- Bacteriophages and microbial interactions
- Diabetes and associated disorders
- Sepsis Diagnosis and Treatment
- Infant Development and Preterm Care
- Metabolism and Genetic Disorders
- Preterm Birth and Chorioamnionitis
- Neonatal Health and Biochemistry
- Pancreatic function and diabetes
- Immune Response and Inflammation
- Theological Perspectives and Practices
- Animal health and immunology
- Congenital Anomalies and Fetal Surgery
- Transplantation: Methods and Outcomes
- Streptococcal Infections and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Diet and metabolism studies
University of Copenhagen
2016-2025
Rigshospitalet
2024
Takeda (Japan)
2024
Aalborg University
2021
Abstract Necrotizing enterocolitis (NEC) is a life-threatening gastrointestinal disorder afflicting preterm infants, which currently unpreventable. Fecal microbiota transplantation (FMT) promising preventive therapy, but the transfer of pathogenic microbes or toxic compounds raise concern. Removal bacteria from donor feces by micropore filtering may reduce this risk bacterial infection, while residual bacteriophages could maintain NEC-preventive effects. We aimed to assess preclinical...
Abstract Background Fecal microbiota transplantation (FMT) and fecal virome (FVT, sterile filtrated donor feces) have been effective in treating recurrent Clostridioides difficile infections, possibly through bacteriophage-mediated modulation of the gut microbiome. However, challenges like variability, costly screening, coupled with concerns over pathogen transfer (incl. eukaryotic viruses) FMT or FVT hinder their wider clinical application less acute diseases. Methods To overcome these...
Optimal nutrition is important after preterm birth to facilitate normal brain development. Human milk rich in sialic acid and infants may benefit from supplementing formula with sialyllactose support neurodevelopment. Using pigs as models, we hypothesized that supplementation improves development birth. Pigs (of either sex) were delivered by cesarean section at 90% gestation fed a diet supplemented an oligosaccharide-enriched whey (n = 20) or lactose for 19 days. Cognitive performance was...
Probiotics are intended to improve gastrointestinal health when consumed. However, the probiotics marketed today only colonize densely populated gut a limited extent. Bacteriophages comprise majority of viruses in human virome and there strong indications that they play important roles shaping microbiome. Here, we investigate use fecal transplantation (FVT, sterile filtrated feces) as mean alter microbiome composition lead way for persistent colonization two types probiotics:...
Fecal filtrate transfer (FFT) is emerging as a safer alternative to traditional fecal microbiota transplantation (FMT) – particularly in the context of necrotizing enterocolitis (NEC), severe gastrointestinal condition affecting preterm infants. Using piglet model, FFT has demonstrated superiority over FMT safety and NEC prevention. Since virtually devoid bacteria, prokaryotic viruses (bacteriophages) are assumed mediate beneficial effects. However, this assumption remains unproven. To...
Abstract Metabolic syndrome encompasses amongst other conditions like obesity and type-2 diabetes is associated with gut microbiome (GM) dysbiosis. Fecal microbiota transplantation (FMT) has been explored to treat metabolic by restoring the GM; however, concerns on accidentally transferring pathogenic microbes remain. As a safer alternative, fecal virome (FVT, sterile-filtrated feces) advantage over FMT in that mainly bacteriophages are transferred. FVT from lean male donors have shown...
Preterm infants have increased risk of neonatal sepsis, potentially inducing brain injury, and they may benefit from early initiation enteral milk feeding. Using preterm pigs as models, we hypothesized that provision bovine colostrum to parentally nourished newborns protects against sepsis neuroinflammation during bloodstream infection. newborn were administered 10 CFU/kg intra-arterial Staphylococcus epidermidis (SE, an opportunistic pathogen often causing in infants), followed by...
Abstract Background Fortification of donor human milk (DHM) is required for optimal growth very preterm infants, but there are concerns more gut dysfunction and necrotizing enterocolitis (NEC) when using formula‐based fortifiers (FFs), especially soon after birth. Intact bovine colostrum (BC) rich in nutrients bioactive factors, protects against NEC pigs. We hypothesized that fortification DHM with BC superior to FFs prevent infections provided shortly Methods Two FF products, Enfamil (ENF;...
Preterm infants are susceptible to bloodstream infection by coagulase-negative staphylococci (CONS) that can lead sepsis. Glucose-rich parenteral nutrition is commonly used support the infants’ growth and energy expenditure but may exceed endogenous regulation during infection, causing dysregulated immune response clinical deterioration. Using a preterm piglet model of neonatal CONS sepsis induced Staphylococcus epidermidis (S. epidermidis) we demonstrate delicate interplay between immunity...
Scope Processing of whey protein concentrate (WPC) for infant formulas may induce modifications with severe consequences preterm newborn development. The study investigates how conventional WPC and a gently processed skim milk‐derived (SPC) affect gut immune development after birth. Methods results Newborn, pigs used as model infants were fed formula containing WPC, SPC, extra heat‐treated SPC (HT‐SPC), or stored HT‐SPC (HTS‐SPC) 5 days. contained no aggregates more native lactoferrin,...
Preterm infants are highly susceptible to late-onset sepsis (LOS) and necrotizing enterocolitis (NEC), but disease pathogenesis specific diagnostic markers lacking. Circulating cell-free DNA (cfDNA) immune cell-derived proteins involved in multiple diseases adults have not been investigated preterm neonates. We explored the relation of circulating neutrophil-associated cfDNA LOS and/or NEC. Using a clinically relevant pig model spontaneous NEC development, we plasma, together with cytokines...
Background: Infants born preterm or small for gestational age (SGA, due to fetal growth restriction) both show an increased risk of neonatal infection. However, it remains unclear how the co-occurrence birth and SGA may affect immunity infection risk. We hypothesized that restricted (FGR) newborns possess impaired immune competence susceptibility systemic sepsis, relative corresponding normal weight (NBW) newborns. Methods: Using pigs as a model infants, gene expression in lipopolysaccharide...
Background. Preterm infants are born with an immature immune system, limited passive immunity and at risk of developing bacteremia sepsis in the postnatal period. We hypothesized that enteral feeding, or without added immunoglobulins, improves clinical response to systemic infection by coagulase negative staphylococci. Methods. Using preterm cesarean delivered pigs as models for infants, we infused live Staphylococcus epidermidis (SE, 5x109 colony forming units per kg) systemically 0-3 days...
Background: Neonatal infection and sepsis are common for preterm infants due to their immature immune system. Early diagnosis is important effective treatment but few early markers of systemic neuro-inflammatory responses in neonates known. We hypothesized that with Gram-positive Staphylococcus epidermidis (SE) induces acute changes proteins plasma cerebrospinal fluid (CSF), potentially affecting the brain neonates. Methods: Using pigs as model infants, CSF samples were collected up 24 h...
The development of necrotizing enterocolitis (NEC), a life-threatening inflammatory bowel disease affecting preterm infants, is connected with gut microbiota dysbiosis. Using piglets as model for infants we recently showed that fecal transplantation (FMT) from healthy suckling piglet donors to newborn decreased the NEC risk. However, in follow-up study using donor stool recruited another farm, this finding could not be replicated. This allowed us donor-recipient dynamics controlled system...
Preterm infants have a high risk of intestinal inflammation which can progress to necrotizing enterocolitis (NEC). The gut microbial colonization commencing at birth is essential for proper maturation, but this process often disrupted in preterm infants, leading dysbiosis and increased developing NEC. Bacteriophages (phages), viruses that specifically infect bacteria, are an important constituent the microbiome protects epithelium against invading bacteria. This study aimed isolate...
Premature infants are highly susceptible to infections that can lead sepsis with life-threatening organ dysfunctions. The clinical practice of high parenteral glucose supply in preterm exacerbate infection outcomes through excessive glycolysis-induced inflammatory response. This turn affect the health vital organs, including brain and kidneys. We hypothesized reduced infected newborns may help protect against pathology these two key organs. Cesarean-delivered pigs were nourished or low...
Preterm infants are susceptible to neonatal sepsis, a syndrome of pro-inflammatory activity, organ damage, and altered metabolism following infection. Given the unique metabolic challenges poor glucose regulatory capacity preterm infants, their intake during infection may have high impact on degree dysregulation damage. Using pig model we previously showed that drastic restriction in supply protects against sepsis via suppression glycolysis-induced inflammation, but results severe...
Necrotizing enterocolitis (NEC) remains a frequent catastrophic disease in preterm infants, and fecal filtrate transfer (FFT) has emerged as promising prophylactic therapy. This study explored the role of virome viability for protective effect FFT. Using ultraviolet (UV) irradiation, we established viral inactivation protocol administered FFT, UV-inactivated FFT (iFFT) or sterile saline orally to piglets at risk experimental NEC. The gut pathology barrier properties were assessed, while...
Energy metabolism and immune response are tightly connected, but it is poorly understood how this interplay regulated in early life to dictate host defense strategy, infection risks severity. This particularly relevant for preterm, low birthweight or otherwise immunocompromised infants, who have poor metabolic control increased of sepsis. Here, we utilized data from the COPSAC2010 cohort with 700 mother-child pairs showed that plasma levels TCA cycle metabolites were associated reduced...