A5101624746- Corneal surgery and disorders
- Advanced biosensing and bioanalysis techniques
- Molecular Biology Techniques and Applications
- Plant-Microbe Interactions and Immunity
- Gene expression and cancer classification
- CRISPR and Genetic Engineering
- Plant Reproductive Biology
- Intraocular Surgery and Lenses
- RNA Interference and Gene Delivery
- Ophthalmology and Visual Impairment Studies
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Pathogenic Bacteria Studies
- Plant Stress Responses and Tolerance
- Polysaccharides and Plant Cell Walls
- Extracellular vesicles in disease
- RNA Research and Splicing
- Nanoplatforms for cancer theranostics
- Plant Parasitism and Resistance
- Glaucoma and retinal disorders
- Bioactive Compounds and Antitumor Agents
- Cytokine Signaling Pathways and Interactions
- Esophageal and GI Pathology
- Ocular Infections and Treatments
- Effects of Radiation Exposure
Chinese Academy of Engineering
2025
Nanjing University
2024
Sichuan University
2018-2024
West China Hospital of Sichuan University
2024
State Key Laboratory of Biotherapy
2020-2024
Citrus Research Institute
2020-2023
Chinese Academy of Agricultural Sciences
2020-2023
Southwest University
2020-2023
Chengdu Third People's Hospital
2020-2021
Southwest Jiaotong University
2020-2021
Abstract Radiotherapy (RT) resistance in head and neck squamous cell carcinoma (HNSCC) significantly hampers local control patient prognosis. This study investigated the efficacy molecular mechanisms of high-energy X-ray-based ultra-high dose rate radiotherapy (UHDR-RT) overcoming RT resistance. The established RT-resistant HNSCC lines animal models were subjected to UHDR-RT or conventional (Conv-RT) via a high-power rhodotron accelerator. Cellular assays assessed malignant phenotype,...
Background To evaluate the changes in corneal endothelium cell density (ECD) and correlated factors after Implantable Collamer Lens (ICL) V4c implantation. Methods In this retrospective, consecutive study, 48 eyes of 25 patients with myopia who underwent ICL implantation were enrolled. Patients followed up for at least 4 years, during which manifest refraction, uncorrected distance visual acuity, corrected acuity (CDVA), intraocular pressure, ECD, anterior chamber depth, volume (ACV), angle...
Immune checkpoint blockade (ICB) is an attractive option in cancer therapy, but its efficacy still less than expected due to the transient and incomplete blocking low responsiveness. Herein, unprecedented programmable unlocking nano-matryoshka-CRISPR system (PUN) targeting programmed cell death ligand 1 (PD-L1) protein tyrosine phosphatase N2 (PTPN2) fabricated for permanent complete highly responsive immunotherapy. While PUN inert at normal physiological conditions, enzyme-abundant tumor...
Abstract Synergistic immune checkpoints blockade (ICB) has the potential to surmount heterogeneity of tumors that are resistant conventional therapeutics and result in better treatment outcomes than monotherapy targetting a single checkpoint. However, current stategies combining multi‐target ICB mainly focused on blockades cell surface which incomplete, temporary, inefficient. Here, multistage sensitive nanocomplex (MUSE) loaded with programmed death‐ligand 1/CD47 multiple targeting...
As the bacterial etiologic agent causing citrus canker (CBC), Xanthomonas citri subsp. (Xcc) seriously impacts plantation and fruit production globally. In an earlier study, we demonstrated that CsBZIP40 can positively impact CBC resistance in sweet orange (Citrus sinensis). However, mechanistic basis for protective benefits conferred by is yet to be delineated. Here, show regulates reactive oxygen species (ROS) homeostasis transgenic overexpressing CsBZIP40. directly binds TGA-box of...
Abstract Purpose To investigate the changes in anterior lens density with a Pentacam after Implantable Collamer Lens (ICL) V4c implantation patients myopia. Methods This prospective case series examined 62 eyes of 32 (mean age, 28.5 ± 5.73 years) myopia or myopic astigmatism ICL implantation. Uncorrected distance vision acuity, corrected acuity (CDVA), manifest refraction, intraocular pressure, chamber depth (ACD), volume (ACV), angle (ACA), endothelial cell and images were obtained pre‐...
Despite promising progress of cancer gene therapy made, these therapeutics were still limited by the diversity sizes and types. CRISPR/dCas9 mediated activation tumor endogenous has shown great potential to surmount hinders genetic varieties during process therapy. However, blood interference along with complicated extra/intracellular microenvironment substantially compromise performance CRISPR/dCas9-based
The authors have developed a novel multiplex detection system that quantitatively measures the expression level of 11 messenger RNAs (mRNAs) directly from cell lysates or tissue homogenates without RNA purification. incorporates branched DNA (bDNA) technology Bayer and bead array platform Luminex. In this study, 21-nt synthetic small interfering (siRNA; specifically designed to knockdown interleukin-8 [IL-8] expression) was delivered into HeLa cells. Using bDNA assay, gene levels were...
Abstract Dysregulation of microRNAs (miRs) is the hallmark triple‐negative breast cancer (TNBC), which closely involved with its growth, metastasis, and recurrence. Dysregulated miRs are promising targets for TNBC therapy, however, targeted accurate regulation multiple disordered in tumors still a great challenge. Here, multi‐targeting on‐demand non‐coding RNA nanoplatform (MTOR) reported to precisely regulate miRs, leading dramatical suppression With assistance long blood circulation,...
Formalin-fixed, paraffin-embedded (FFPE) tissues represent an important source of archival materials for gene expression profiling. We report here the development a modified branch DNA assay that allows direct quantification messenger RNA (mRNA) transcripts in homogenates from FFPE tissue sections without need isolation and reverse transcription into cDNA. Formalin fixation essentially has no effect on assay, degradation only marginally reduces signal by 2- to 3-fold. Under same conditions,...
To observe the changes in corneal densitometry (CD) with a Pentacam after implantable collamer lens (ICL) V4c implantation myopia patients, and to investigate potential influencing factors.We reviewed 65 eyes of 33 patients (mean age, 29.08±5.54 years) or myopic astigmatism who underwent ICL implantation. Clinic parameters images topography CD were obtained pre- postoperatively.After an average 52±2.2 months follow-up, efficacy safety indexes assessed at last follow-up 1.03±0.18 1.21±0.21,...
Purpose To investigate the effects of ocular residual astigmatism (ORA) and target-induced (TIA) on efficacy toric implantable collamer lens (TICL) with central hole for myopic correction. Methods Retrospective case series. One hundred eighteen eyes implanted a TICL (V4c) from 118 patients were included. Subjective refraction corneal topography examined preoperatively, at 1 12 months postoperatively. The divided into low-ORA ( ≤ 0.5 D) high-ORA (>0.5 groups based vector analysis,...
Abstract Aims To compare the vault performance between implantable collamer lens (ICL) V4c and Toric ICL (TICL) after implantation to investigate affecting factors. Methods Sixty‐eight eyes from 34 patients with myopia or astigmatism who underwent of TICL in one eye (group A) identically sized B) contralateral were included. Mean follow‐up time 7.58 ± 1.63 months (range: 6–10 months). Vault was compared two groups correlations age, preoperative ocular biometric measurements analysed....
The present study was designed to serve as a comprehensive analysis of Citrus sinensis (C. sinensis) pectin acetylesterases (CsPAEs), and assess the roles these PAEs involved in development citrus bacterial canker (CBC) caused by Xanthomonas citri subsp. (Xcc) infection. A total six CsPAEs were identified genome C. sinensis, with genes being unevenly distributed across chromosomes 3, 6, 9, unassembled scaffolds. subset found be responses Xcc In particular, CsPAE2 associated such infections,...
Purpose: To evaluate the characteristics of peripheral anterior chamber measurements by Pentacam after posterior implantable collamer lenses (ICL) and toric ICL (TICL) with central hole (V4c) implantation. Methods: Prospective, non-randomized consecutive case series. Forty-six patients undergoing implantation in one eye (Group A) identically sized TICL contralateral B) Refractive Surgery Center Eye ENT Hospital Fudan University were prospectively included. According to ICL/TICL size, these...
As a member of the signal transducer and activator transcription (STAT) family, STAT3 plays critical role in several biological pathways such as cell proliferation, migration, survival, differentiation. Due to abnormal continuous activation tumors, inhibition has emerged an attractive approach for treatment various cancer cells. Herein, we report series novel inhibitors based on benzo[b]thiophene 1,1-dioxide scaffold evaluated their anticancer potency. Among them, compound 8b exhibited best...