A5003617597- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Streptococcal Infections and Treatments
- Milk Quality and Mastitis in Dairy Cows
- Probiotics and Fermented Foods
- RNA and protein synthesis mechanisms
- Antimicrobial Resistance in Staphylococcus
- Clostridium difficile and Clostridium perfringens research
- Mycobacterium research and diagnosis
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Advanced Drug Delivery Systems
- Yersinia bacterium, plague, ectoparasites research
- RNA Interference and Gene Delivery
- Bacterial Identification and Susceptibility Testing
- Infant Nutrition and Health
- Carbohydrate Chemistry and Synthesis
- Toxoplasma gondii Research Studies
- African Botany and Ecology Studies
- Salmonella and Campylobacter epidemiology
- Infections and bacterial resistance
- Antibiotic Resistance in Bacteria
- CRISPR and Genetic Engineering
Jilin University
2017-2025
Enterococcus faecalis is becoming an increasingly important opportunistic pathogen worldwide, especially because it can cause life-threatening nosocomial infections. Treating E. infections has become difficult of the prevalence multidrug-resistant strains. Because bacteriophages show specificity for their bacterial hosts, there been a growth in interest using phage therapies to combat rising incidence In this study, we isolated new lytic phage, EF-P29, which showed high efficiency and broad...
Klebsiella pneumoniae (K. pneumoniae) spp. are important nosocomial and community-acquired opportunistic pathogens, which cause various infections. We observed that K. strain K7 abruptly mutates to rough-type phage-resistant phenotype upon treatment with phage GH-K3. In the present study, mutant named K7RR showed much lower virulence than K7. Liquid chromatography-tandem mass spectrometry (LC-MS-MS) analysis indicated WcaJ two undefined glycosyltransferases (GTs)- GT-1, GT-2- were found be...
Klebsiella pneumoniae ( K. ) is an important nosocomial and community acquired opportunistic pathogen which causes various infections. The emergence of multi-drug resistant (MDR) carbapenem-resistant hypervirulent (CR-hvKP) has brought more severe challenge to the treatment infection. In this study, a novel bacteriophage that specifically infects was isolated named as vB_KpnM_P-KP2 (abbreviated P-KP2). biological characteristics P-KP2 bioinformatics its genome were analyzed, then therapeutic...
Phage-derived lysins can hydrolyse bacterial cell walls and show great potential for combating Gram-positive pathogens. In this study, the of LysEF-P10, a new lysin derived from isolated Enterococcus faecalis phage EF-P10, as an alternative treatment multidrug-resistant E. infections, was studied. LysEF-P10 shares only 61% amino acid identity with its closest homologues. Four proteins were expressed: cysteine, histidine-dependent amidohydrolase/peptidase (CHAP) domain (LysEF-P10C), putative...
Most staphylococcal species are major causes of health care- and community-associated infections. In particular, Staphylococcus aureus is a common dangerous pathogen, epidermidis ubiquitous skin commensal opportunistic pathogen. Treatment infections caused by staphylococci has become more difficult because the emergence multidrug-resistant strains as well biofilm formation. this study, we found that all tested S. , haemolyticus hominis were sensitive to phage lysin LysGH15 (MICs ranging from...
Staphylococcus aureus (S. aureus) is a common and dangerous pathogen that causes various infectious diseases. Skin damage, such as burn wounds, are at high risk of colonization infection, which increases morbidity mortality. The phage lysin LysGH15 exhibits highly efficient lytic activity against methicillin-resistant S. (MRSA) methicillin-susceptible (MSSA) strains. Apigenin (api) significantly decreases haemolysis rabbit erythrocytes caused by shows anti-inflammatory function. api were...
Bacteriophage can be used as an alternative or complementary therapy to antibiotics for treating multidrug-resistant bacterial infections. However, the rapid emergence of resistant host variants during phage treatment has limited its therapeutic applications. In this study, a potential phage-resistant mechanism Klebsiella pneumoniae was revealed. After GH-K3 treatment, smooth-type colony, named K7RB, obtained from K. K7 culture. Treatment with IO4- and/or proteinase K indicated that...
Staphylococcus aureus (S. aureus) is one of the most important zoonotic pathogens and can be transmitted to humans through meat diet routes, causing necrotising pneumonia. This study investigated therapeutic effect bacteriophage lysin LysGH15 on pneumonia in rabbit model caused by S. aureus. In vitro experiments, 50 μg/mL not only significantly reduced viable count (approximately 3.24 × 106 CFU/g) chicken stored at 4°C for 48 h but also effectively thawed 30°C, with reductions approximately...
Phaffia rhodozyma (PR) is a candidate microbial strain for the production of natural astaxanthin. Astaxanthin and other carotenoids have significant antioxidant effects, which can delay aging prevent tumors. However, so far, there no effective convenient method to comprehensively reflect quality PR, also gap in research on potential antiaging effect PR. In this study, 15 batches PR samples collected China were analyzed using high‐performance liquid chromatography (HPLC) fingerprinting. A...
Aerococcus viridans is an opportunistic pathogen that clinically associated with various human and animal diseases. In this study, the first identified A. phage, vB_AviM_AVP (abbreviated as AVP), was isolated studied. AVP belongs to family Myoviridae. harbors a double-stranded DNA genome length of 133,806 bp G + C content 34.51%. The sequence showed low similarity (<1% identity) those other phages, bacteria, or organisms in database. Among 165 predicted open reading frames (ORFs), there were...
Yersinia enterocolitica is generally considered an important food-borne pathogen worldwide, especially in the European Union. A lytic phage X1 (Viruses; dsDNA viruses, no RNA stage; Caudovirales; and Myoviridae) was isolated. Phage showed a broad host range could effectively lyse 27/51 Y. strains covering various serotypes that cause yersiniosis humans animals (such as serotype O3 O8). The genome of this sequenced analyzed. No toxin, antibiotic-resistance or lysogeny related modules were...
Abstract Background Ribosomal protein SA (RPSA) of human brain microvascular endothelial cells (HBMECs) can transfer from the cytosol to cell surface and act as a receptor for some pathogens, including Streptococcus suis serotype 2 (SS2), zoonotic pathogen causing meningitis in pigs humans. We previously reported that SS2 virulence factor enolase (ENO) binds RPSA on HBMECs induces apoptosis. However, mechanism activates translocation ENO-mediated HBMEC apoptosis is unclear. Results Here, we...
Salmonella enterica subsp. serovar Abortusequi is a frequently reported pathogen causing abortion in mares. In this study, the preventive and therapeutic effects of phage PIZ SAE-01E2 against S mouse model were investigated. Phage was stable at different temperatures (4 to 70°C) pH values (pH 4 10) could lyse majority serogroup O:4 O:9 strains tested (25/28). There no lysogeny-related, toxin, or antibiotic resistance-related gene genome SAE-01E2. All these characteristics indicate that has...
Aeromonas veronii (A. veronii) is a pathogenic that can infect human, animal and aquatic organisms, in which poses huge threat to the health of many organisms such as Cyprinus carpio. In this study, Lactobacillus casei (L. casei) strain CC16 was used antigen deliver carrier fused with cholera toxin B subunit (CTB) an adjuvant construct recombinant L. pPG-Aha1/Lc CC16(surface-displayed) pPG-Aha1-CTB/Lc expressing Aha1 protein A. veronii, respectively. And immune responses carpio by oral route...
Bovine mastitis is the most common and costly disease affecting dairy cattle throughout world. Enterococcus faecalis one of environmental origin mastitis-causing pathogens. The treatment bovine primarily based on antibiotics. Due to negative impact developing antibiotic resistance adverse effects soil water environments, trend toward use nonantibiotic treatments increasing. Phages may represent a promising alternative strategy. However, it unknown whether phages have therapeutic E....
Streptococcus suis (S. suis) is a swine pathogen that can cause sepsis, meningitis, endocarditis, and other infectious diseases; it also zoonotic has caused global surge in fatal human infections. The widespread prevalence of multidrug-resistant S. strains the decline novel antibiotic candidates have necessitated development alternative antimicrobial agents. In this study, AVPL, Aerococcus viridans (A. viridans) phage lysin, was found to exhibit efficient bactericidal activity broad lytic...
Salmonella enterica serovar Typhimurium (S. Typhimurium) is one of the common causes human colitis. In present study, two lytic phages vB_SenS-EnJE1 and vB_SenS-EnJE6 were isolated therapeutic effect combination faecal microbiota transplantation (FMT) on S. Typhimurium-induced mouse colitis was investigated. The characteristics genome analysis indicated that they are suitable for phage therapy. Results showed lysis 41/54 strains serotype O4, 46/54 serotypes O4 O9. Severe inflammatory...
Bovine mastitis, an inflammatory disease that occurs frequently in early lactation or the dry period, is primarily caused by bacterial infections. There growing evidence Aerococcus viridans (A. viridans) becoming important cause of bovine mastitis. The treatment mastitis based on antibiotics, which not only leads to a large economic burden but also development antibiotic resistance. On other hand, bacteriophages present promising alternative strategy. object this study was evaluate potential...
Salmonella enterica serovar Typhimurium ( S. typhimurium ) is one of the most important foodborne pathogens that causes colitis in humans.
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