A5005336389- Bacteriophages and microbial interactions
- Biochemical and Structural Characterization
- Genomics and Phylogenetic Studies
- Microbial infections and disease research
- Antimicrobial Peptides and Activities
- Clostridium difficile and Clostridium perfringens research
- Bacterial Genetics and Biotechnology
- Gut microbiota and health
- Pharmacological Effects of Natural Compounds
- Probiotics and Fermented Foods
- Statistical Methods and Inference
- Flavonoids in Medical Research
Jilin University
2015-2017
Enterococcus faecalis is becoming an increasingly important opportunistic pathogen worldwide, especially because it can cause life-threatening nosocomial infections. Treating E. infections has become difficult of the prevalence multidrug-resistant strains. Because bacteriophages show specificity for their bacterial hosts, there been a growth in interest using phage therapies to combat rising incidence In this study, we isolated new lytic phage, EF-P29, which showed high efficiency and broad...
ABSTRACT Pneumonia is one of the most prevalent Staphylococcus aureus -mediated diseases, and treatment this infection becoming challenging due to emergence multidrug-resistant S. , especially methicillin-resistant (MRSA) strains. It has been reported that LysGH15, lysin derived from phage GH15, displays high efficiency a broad lytic spectrum against MRSA apigenin can markedly diminish alpha-hemolysin . In study, combination therapy LysGH15 was evaluated in vitro mouse pneumonia model. No...
Abstract The lysin LysGH15, derived from the staphylococcal phage GH15, exhibits a wide lytic spectrum and highly efficient activity against methicillin-resistant Staphylococcus aureus (MRSA). Here, we found that LysGH15 did not induce resistance in MRSA or methicillin-sensitive S. (MSSA) strains after repeated treatment. Although triggered generation of LysGH15-specific antibodies mice, these block vitro (nor binding capacity LysGH15). More importantly, when antibody titre was highest mice...
Phage-derived lysins can hydrolyse bacterial cell walls and show great potential for combating Gram-positive pathogens. In this study, the of LysEF-P10, a new lysin derived from isolated Enterococcus faecalis phage EF-P10, as an alternative treatment multidrug-resistant E. infections, was studied. LysEF-P10 shares only 61% amino acid identity with its closest homologues. Four proteins were expressed: cysteine, histidine-dependent amidohydrolase/peptidase (CHAP) domain (LysEF-P10C), putative...
Holins are phage-encoded hydrophobic membrane proteins that spontaneously and non-specifically accumulate form lesions in the cytoplasmic membrane. The ORF72 gene (also designated HolGH15) derived from genome of Staphylococcus aureus phage GH15 was predicted to encode a protein. An analysis indicated protein encoded by HolGH15 potentially consisted two transmembrane helices. This exhibited structural characteristics class II holins belonged phage_holin_1 superfamily. Expression Escherichia...