Abstract Our preliminary work has revealed that vitamin D receptor (VDR) activation is protective against cisplatin induced acute kidney injury (AKI). Ferroptosis was recently reported to be involved in AKI. Here this study, we investigated the internal relation between ferroptosis and effect of VDR By using inhibitor ferrostatin-1 measurement ferroptotic cell death phenotype both vivo vitro AKI model, observed decreased blood urea nitrogen, creatinine, tissue by ferrostatin-1, hence...

The aim of this study was to assess the significance expression hypoxia-inducible factor-1alpha (HIF-1alpha) and associated proteins in pancreatic ductal adenocarcinoma (PDA) their impact on prognosis. Expression HIF-1alpha, vascular endothelial growth factor (VEGF), glucose transporter-1 (Glut-1), survivin, CD34 Ki-67 apoptotic cells demonstrated by immunohistochemistry or TUNEL 58 PDAs 20 normal tissue samples. Our results show positivity VEGF, Glut-1 survivin 70.7%, 77.6%, 67.2% 84.5%...

BACKGROUND Metastasis is the primary cause of prostate cancer (PCa) lethality and poses a huge clinical obstacle. Lipocalin 2 (LCN2), member lipocalin family, aberrantly expressed in some human cancers has been implicated progression tumors. However, role LCN2 metastatic capacity poorly understood. METHODS expression was examined by RT-qPCR and/or immunoblotting tissue specimens cell lines LNCaP, C4-2, 22RV1, PC3, DU-145, PC3MM2. protein level serum samples determined ELISA....

The immune environment in primary tumor has a profound impact on immunotherapy. However, the clinical relevance of hepatocellular carcinoma (HCC) is largely unknown. Here, profile and its response HCC were investigated. gene expression profiles 569 HCCs from three cohorts (The Cancer Genome Atlas, TCGA, n = 257; Gene Expression Omnibus, GEO, 170; International Consortium, ICGC, 142) used current study. Five subtypes (C1-C5) responsible for global genes identified at stage I/II. It was found...

The dreadful prognosis of hepatocellular carcinoma (HCC) is primarily due to the low early diagnosis rate, rapid progression, and high recurrence rate. Valuable prognostic biomarkers are urgently needed for HCC. In this study, microarray data were downloaded from GSE14520, GSE22058, International Cancer Genome Consortium (ICGC), Atlas (TCGA). Differentially expressed genes (DEGs) identified among ICGC databases. Weighted gene co-expression network analysis (WGCNA) was used establish modules...

Background: Nowadays, clinical treatment outcomes of patients with hepatocellular carcinoma (HCC) has been improved. However, due to the complexity molecular mechanisms, recurrence rate and mortality in HCC inpatients are still at a high level. Therefore, there is an urgent need screening biomarkers show therapeutic effects improve prognosis. Methods: In this study, we aim establish gene signature that can predict prognosis by downloading analyzing RNA sequencing data information from 3...

Increasing evidences have documented that microRNAs (miRNAs) act as oncogenes or tumor suppressors in a variety types of cancer. The discovery associated miRNAs serum patients gives rise to extensive investigation circulating many human cancers which support the use plasma/serum noninvasive means cancer detection. However, aberrant expression and bladder are less reported. We used Taqman probe stem-loop real-time PCR accurately measure levels miR-99a cell lines, 100 pairs tissues, adjacent...

Health literacy (HL) has become an important public health issue and is receiving growing attention. However, the HL levels of military college students in China have never been analyzed. This study aimed to investigate related associate factors Chongqing, China. Data was obtained with "Chinese Citizen Literacy Questionnaire (2012 edition)" from 3183 aged 16-28 years at Chongqing December 2015. A total score ≥80 points determined adequate HL, level defined as proportion who had out number...

Abstract BACKGROUND Slug is a transcription factor of the Snail/Slug zinc‐finger family and implicated in metastasis tumors, but its role cell proliferation prostate cancers unclear. METHODS Expression level other genes was examined by Western blot, RT‐PCR, QPCR analyses. The forced expression mediated retroviruses adenoviruses. downregulated shRNA. Cell growth measured MTT assay quick assay. RESULTS Here, we demonstrated that elevated mouse human cancer lines LNCaP, PC‐3, 22RV1. Forced...

High-mobility group A1 (HMGA1) has been suggested to play a significant role in tumor progression, but little is known about the accurate significance of HMGA1 non-small cell lung cancer (NSCLC) patients. The aim this study was identify NSCLC. expression status observed initially NSCLC by Gene Expression Omnibus (GEO). messenger RNA (mRNA) and protein examined adjacent normal tissues through real-time PCR immunohistochemistry. Meanwhile, relationship levels with clinical features prognosis...

Abstract The mechanisms underlying the development and progression of colon cancer are not fully understood. Herein, Src kinase associated phosphoprotein 1 (SKAP1), an immune cell adaptor, is identified as a novel cancer‐related gene. SKAP1 expression significantly increased in cells. High levels independently predictive poor survival patients with cancer. Notably, cells exerted significant tumor‐promoting effect vivo rather than vitro. Screening tumor‐infiltrating revealed involvement...

Though prostate cancer (PCa) has slow progression, the hormone refractory (HRCP) and metastatic entities are substantially lethal lack effective treatments. Transcription factor Slug is critical in regulating metastases of various tumors including PCa. Here we studied targeted therapy against using combination 3 drugs targeting pathways respectively converging via further PCa metastasis. Using vitro assays confirmed that up-regulation incurred inhibition E-cadherin was anti-metastatic,...

Background Calcium oxalate monohydrate (COM) is the major crystalline component in kidney stones and its adhesion to renal tubular cells leads injury. However, COM-induced toxic effects remain ambiguous. MicroRNAs (miRNAs) play an important role gene regulation at posttranscriptional levels. Objective The present study aimed assess potential changes microRNAs of proximal response calcium crystals. Methodology Lactate dehydrogenase (LDH) activity DAPI staining were used measure HK-2 exposed...

Background . Gastric cancer (GC) is one of the leading causes cancer‐related mortality worldwide. There are great geographical differences in incidence GC, and somatic mutation rates driver genes also different. The present study aimed at screening core prognosis‐related candidate Chinese gastric population based on integrated bioinformatics for early diagnosis prognosis GC. Methods In study, differentially expressed (DEGs) GC were identified using four microarray datasets from Gene...

Sirtuin 1 (SIRT1), a well-characterized NAD+-dependent histone deacetylase, is generally up-regulated in gastrointestinal cancers. However, the prognostic value of SIRT1 cancer remains inconclusive. Therefore, we report meta-analysis association expression with overall survival (OS) cancer. PubMed was systematically searched for studies evaluating and OS patients Fifteen (six colorectal cancer, three hepatocellular carcinoma, gastric one each pancreatic esophageal squamous cell...

Hepatocellular carcinoma (HCC) is one of the most malignant types cancer, and associated with high recurrence rates a poor response to chemotherapy. Immune signatures in microenvironment HCC have not been well explored systematically. The aim present study was identify prognostic immune build nomogram for use clinical evaluation. Using bioinformatics analysis, RNA‑seq data overall survival (OS) information on 370 cases from TCGA 232 ICGC were analyzed. differential expression select genes,...

Esophageal cancer is characterized by rapid clinical progression and poor prognosis, due to early-stage invasion of adjacent tissues metastasis. Tissue factor pathway inhibitor-2 (TFPI-2) has been implicated as a metastasis-associated gene in many types tumors. Here we describe the potential involvement TFPI-2 development esophageal carcinoma. Western blotting revealed that was downregulated 75% carcinomas most carcinoma cell lines. Immunohistochemistry significantly tumor lymph node...

Long non-coding RNAs (lncRNAs), which have emerged as important regulatory RNA molecules that been implicated in carcinogenesis and cancer progression, may also serve novel potential biomarkers for diagnosis prognosis. Our previous analysis has identified the lncRNA GHRLOS, ghrelin antisense strand gene, one of hub genes co-expression network differentially expressed lncRNAs/mRNAs colorectal (CRC). Here, we further evaluate expression GHRLOS CRC explore its clinical significance. The 366...

E3 ubiquitin ligases (E3s), the second most common cancer-related functional protein family, play vital roles in multiple tumors. However, their importance prognosis and immunotherapy of lung adenocarcinoma (LUAD) is not clear. First, utilizing data from The Cancer Genome Atlas (TCGA), we comprehensively assessed expression profile immunological association 13 E3s LUAD patients. Consequently, MARCH1 was considered a candidate for further study. Second, several algorithms were applied to...