A5100335420- Congenital heart defects research
- Developmental Biology and Gene Regulation
- RNA modifications and cancer
- RNA Research and Splicing
- Cellular transport and secretion
- Cancer-related molecular mechanisms research
- Pancreatic function and diabetes
- Zebrafish Biomedical Research Applications
- Wnt/β-catenin signaling in development and cancer
- Endoplasmic Reticulum Stress and Disease
- Nuclear Structure and Function
- Immune Cell Function and Interaction
- melanin and skin pigmentation
- Protein Kinase Regulation and GTPase Signaling
- Genomics and Chromatin Dynamics
- Retinal Development and Disorders
- Angiogenesis and VEGF in Cancer
- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Microtubule and mitosis dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- T-cell and B-cell Immunology
- Immune cells in cancer
- Calcium signaling and nucleotide metabolism
- Hippo pathway signaling and YAP/TAZ
Cornell University
2024
Weill Cornell Medicine
2024
University of Electronic Science and Technology of China
2024
Center for Life Sciences
2015-2022
Tsinghua University
2015-2022
King Center
2017
Nuclear pore complexes (NPCs) are channels for nucleocytoplasmic transport of proteins and RNAs. However, it remains unclear whether composition, structure, permeability NPCs dynamically change during the cleavage period vertebrate embryos affect embryonic development. Here, we report that comprehensive NPC maturity (CNM) controls onset zygotic genome activation (ZGA) zebrafish early embryogenesis. We show more nucleoporin recruited to assembled into with development, resulting in...
Abstract Directed cell migration is driven by the front–back polarization of intracellular signalling 1–3 . Receptor tyrosine kinases and other inputs activate local signals that trigger membrane protrusions at front 2,4–6 Equally important a long-range inhibitory mechanism suppresses back to prevent formation multiple fronts 7–9 However, identity this unknown. Here we report endoplasmic reticulum–plasma (ER–PM) contact sites are polarized in single collectively migrating cells. The...
The non-canonical Wnt/Ca2+ signaling pathway plays important roles in embryonic development, tissue formation and diseases. However, it is unclear how the Wnt ligand-stimulated, G protein-coupled receptor Frizzled activates phospholipases for calcium release. Here, we report that zebrafish/human phosphatidylinositol transfer protein Sec14l3/SEC14L2 act as GTPase proteins to transduce signals from phospholipase C (PLC). Depletion of sec14l3 attenuates responsive activity causes convergent...
Background Glioma is a highly heterogeneous malignancy of the central nervous system. This heterogeneity driven by various molecular processes, including neoplastic transformation, cell cycle dysregulation, and angiogenesis. Among these biomolecular events, inflammation stress pathways in development driving factors glioma have been reported. However, mechanisms under response remain unclear, especially from spatial aspect. Methods study employed single-cell RNA sequencing (scRNA-seq)...
Abstract Vascular endothelial growth factor (VEGF) regulates vasculogenesis by using its tyrosine kinase receptors. However, little is known about whether Sec14-like phosphatidylinositol transfer proteins (PTP) are involved in this process. Here, we show that zebrafish sec14l3 , one of the family members, specifically participates artery and vein formation via regulating angioblasts subsequent venous progenitors’ migration during vasculogenesis. defects caused depletion partially rescued...
Abstract Histone recognition constitutes a key epigenetic mechanism in gene regulation and cell fate decision. PHF14 is conserved multi-PHD finger protein that has been implicated organ development, tissue homeostasis, tumorigenesis. Here we show reads unmodified histone H3(1–34) through an integrated PHD1-ZnK-PHD2 cassette (PHF14PZP). Our binding, structural HDX-MS analyses revealed feature of bipartite recognition, which PHF14PZP utilizes two distinct surfaces for concurrent yet separable...
The zebrafish sensory posterior lateral line (pLL) has become an attractive model for studying collective cell migration and morphogenesis. Recent studies have indicated that chemokine, Wnt/β-catenin, Fgf, Delta-Notch signaling pathways participate in regulating pLL development. However, it remains unclear whether TGFβ pathway is involved Here we report a critical role of TGFβ1 morphogenesis the primordium (pLLP). tgfβ1a gene abundantly expressed primordium. Knockdown or knockout leads to...
During embryonic gastrulation, coordinated cell movements occur to bring cells their correct position.Among them, epiboly produces the first distinct morphological changes, which is essential for early development of zebrafish.Despite its fundamental importance, little known understand underlying molecular mechanisms.By generating maternal mutant lines with CRISPR/Cas9 technology and using morpholino knockdown strategy, we showed that Alkbh4 depletion leads severe defects in...
Nodal proteins provide crucial signals for mesoderm and endoderm induction. In zebrafish embryos, the nodal genes ndr1/squint ndr2/cyclops are implicated in mesendoderm It remains elusive how ndr1 ndr2 expression is regulated spatiotemporally. Here we investigated regulation of using Mhwa mutants that lack maternal dorsal determinant Hwa with deficiency β-catenin signaling, Meomesa Eomesodermin A (Eomesa), Meomesa;Mhwa double mutants, signaling inhibitor SB431542. We show completely...
Abstract Embryonic axis formation is essential for patterning and morphogenesis in vertebrates tightly regulated by the dorsal organizer. Previously, we demonstrated that maternally derived Huluwa (Hwa) acts as a determinant, dictating activating β-catenin signaling zebrafish Xenopus . However, mechanism of activation fine regulation Hwa protein remains unclear. Through candidate screening identified mutation at Ser168 PPNSP motif dramatically abolishes its axis-inducing activity....
Activation of nodal genes is critical for mesoderm and endoderm induction. Our previous study reported that zebrafish ndr1 / squint ndr2 cyclops are coordinately regulated by maternal Eomesa, Hwa-activated β-catenin (Hwa/β-catenin) signaling, Nodal autoregulation (Nodal/Smad2) signaling. However, the exact contribution underlying mechanisms still elusive. Here, we applied “causal inference” to evaluate causal between independent dependent variables, found Hwa/β-catenin Smad2 cause...
Highlights● NPCs matures gradually in the zebrafish embryo after fertilization● Maternal TFs cannot transport into nuclei efficiently embryos before ZGA● Nuclear pore complex maturity controls nuclear and ZGA onset● Dysfunction of complexes delays embryonic development