A5049540949- Parasitic infections in humans and animals
- Wnt/β-catenin signaling in development and cancer
- Parasitic Infections and Diagnostics
- Cell Adhesion Molecules Research
- Bladder and Urothelial Cancer Treatments
- Congenital Anomalies and Fetal Surgery
- Epigenetics and DNA Methylation
- Immune Response and Inflammation
- Angiogenesis and VEGF in Cancer
- Monoclonal and Polyclonal Antibodies Research
- Tissue Engineering and Regenerative Medicine
- Parasite Biology and Host Interactions
- Pancreatic function and diabetes
- Nanoparticle-Based Drug Delivery
- Coccidia and coccidiosis research
- Nuclear Receptors and Signaling
- Viral gastroenteritis research and epidemiology
- Diabetes and associated disorders
- Urinary and Genital Oncology Studies
- Cancer-related gene regulation
- NF-κB Signaling Pathways
- Growth Hormone and Insulin-like Growth Factors
- Galectins and Cancer Biology
- Atherosclerosis and Cardiovascular Diseases
- Neonatal Respiratory Health Research
Ohio University
2014-2025
University of Michigan
2018
William Harvey Research Institute
2018
University of North Carolina at Chapel Hill
2018
Florida State University
2014
Philadelphia College of Osteopathic Medicine
2014
Biotechnology Institute
2005
Universidad de la República
1996-2004
Centro de Ornitología y Biodiversidad
2003
Universidad La República
2001
Atherosclerosis is an inflammatory disease involving the accumulation of macrophages in intima. Wnt5a a noncanonical member Wnt family secreted glycoproteins. Recently, human have been shown to express upon stimulation with bacterial pathogens vitro and granulomatous lesions lung Mycobacterium tuberculosis-infected patients. expression has also liked Toll-like receptor-4 (TLR-4), innate immune receptor implicated atherosclerosis. These observations, along fact that involved cell migration...
Atherosclerosis (ATH) is a chronic inflammatory disease that involves cascades of signaling events mediated by various effector proteins. Here we sought to determine if the expression Wnt5a, secreted glycoprotein, altered in discrete regions arterial plaque. Atherosclerotic plaque tissues from 14 human subjects undergoing elective carotid endarterectomy were used this study. Immunohistochemistry and laser capture microdissection combined with quantitative real-time PCR Wnt5a Toll-like...
High basal levels of TLR3 and Wnt5a RNA are present in papillary thyroid carcinoma (PTC) cell lines consistent with their overexpression colocalization PTC cells vivo. This is not the case thyrocytes from normal tissue follicular (FC) or anaplastic (AC) tissues. The basally expressed functional as evidenced by ability double-strand (polyinosine-polycytidylic acid) to significantly increase activity transfected NF-kappaB IFN-beta luciferase reporter genes two end products signaling, CXCL10....
To evaluate whether (a) Wnt5a expression in pancreatic cancer and malignant melanoma cells might be associated with constitutive levels of Toll-like receptor 3 (TLR3) and/or TLR3 signaling; (b) phenylmethimazole (C10), a novel TLR signaling inhibitor, could decrease together cell growth migration; (c) the efficacy C10 as potential inhibitor vivo.We used variety molecular biology techniques including but not limited to PCR, Western blotting, ELISA presence constitutively activated TLR3/Wnt5a...
Clostridioides difficile (C. difficile) is a leading cause of hospital-associated diarrhea, primarily due to gut dysbiosis following antibiotic use. Probiotics have been found provide several benefits hosts via modulation the microbiota and their metabolites. However, till now, no conventional probiotics clearly proven be an effective prophylactic option for CDI prevention. Therefore, more studies on developing specific probiotic candidates targeting improving diversity administrated are...
Objective: Wnt5a is a secreted glycoprotein highly present in atherosclerotic lesions.Uptake of oxidized-low density lipoprotein (ox-LDL) by monocytes/macrophages plays critical role atherosclerosis.The objective this study was to determine if mRNA expression correlates with the severity lesions, and if, ox-LDL can induce macrophages.Methods: tissue sections from carotid arteries patients undergoing endarterectomy quantified via RT-PCR correlated plaque severity.Human monocyte-derived...
The expression of certain endothelial cell adhesion molecules (ECAMs) is increased in the vasculature inflamed bowel (e.g., colitis), thereby providing an opportunity for targeted drug delivery. We recently demonstrated that biodegradable particles conjugated with ligands to ECAMs exhibit significant selective ECAM expressing endothelium. In present study, we used a murine model colitis determine whether poly(lactic acid)-poly(ethylene glycol) VCAM-1 ligand (alpha-V) enhanced colitic...
Abstract The increased expression of VCAM‐1 on endothelial segments within plaque regions could be used as a target to deliver polymeric drug carriers selectively sites atherosclerosis. We probed the hypothesis that particles conjugated with ligand for exhibit selective and avid adhesion Particles made from polystyrene or biodegradable polymer poly(sebacic acid)‐block‐polyethylene glycol (PSA‐PEG) were an antibody (α‐VCAM‐1) IgG (negative control). injected into jugular vein ApoE −/− (a...
Group B coxsackieviruses (CVBs) are involved in triggering some cases of type 1 diabetes mellitus (T1DM). However, the molecular mechanism(s) responsible for this remain elusive. Toll-like receptor 3 (TLR3), a that recognizes viral double-stranded RNA, is hypothesized to play role virus-induced T1DM, although hypothesis yet be substantiated. The objective study was directly investigate TLR3 CVB-triggered T1DM nonobese diabetic (NOD) mice, mouse model human widely used both spontaneous...
A helminthological survey of the intestinal parasites in stray dogs was conducted urban and suburban area Tacuarembó, Uruguay, during winter time. Eighty captured city were necropsied. Seventy nine (98.8 %) positive for helminth infection. seven (96.3 parasitized by hookworms. Two species hookworms found: Ancylostoma caninum 96.3 % A. braziliense 49.4 %. This is first report prevalence Uruguay. Considering that incidences human cutaneous larva migrans caused migration larvae restricted...
This article focuses on the initiation pathway of mucin-type O-glycosylation in helminth parasites. The presence GalNAc-O-Ser/Thr structure, also known as Tn antigen, a truncated determinant related to aberrant glycosylation mammal cells, and activity UDP-GalNAc:polypeptide N-acetyl-galactosaminyltransferase (ppGaNTase), enzyme responsible for its synthesis, were studied species from major taxonomic groups. reactivity was determined extracts Taenia hydatigena, Mesocestoides corti, Fasciola...
The pathogenesis of atherosclerosis is complex, evolves, and involves many cell types. Macrophages vascular smooth muscle cells (VSMCs) are critically involved in development progression. Several studies have shown that WNT5A protein abundantly expressed human atherosclerotic lesions; however, the mechanism role WNT signaling pathway activation not clearly known. Using THP-1 derived macrophages, aortic VSMC cells, we evaluated vitro how oxidized low-density lipoprotein (oxLDL) interact these...
This investigation aimed to design a strategy for echinococcosis control in Santana do Livramento county, an endemic area state of Rio Grande Sul (Brazil). Fecal samples from 65 dogs were obtained urban, suburban and rural areas. Purging with Arecoline Bromhidrate (AB) was done visualize Echinococcus granulosus, Enzyme Linked Immunosorbent Assay (ELISA) performed detect parasite coproantigen. Samples at the beginning end treatment Praziquantel. A third fecal sampling also areas four months...
Wnt binding to cell surface receptors can activate a 'canonical' pathway that increases cellular beta-catenin or 'noncanonical' Ca(++) which increase protein kinase C (PKC) activity. Although components of both Wnt/beta-catenin-signaling pathways exist in thyrocytes, their biological role is largely unknown. In evaluating the signaling differentiated FRTL-5 thyroid cells, we showed TSH increased canonical Wnt-1 but, surprisingly, decreased active form beta-catenin. Transient overexpression...
// Anthony L. Schwartz 1 , Eric Dickerson 2 Nilesh Dagia 3 Ramiro Malgor and Kelly D. McCall 4 Athencion Biotechnology Corporation, Suite 100, Marietta, OH 45750, USA Department of Biomedical Sciences, Appalachian Rural Health Institute, Diabetes Research Center, College Osteopathic Medicine, Ohio University, Athens, 45701, Oxygen Healthcare Pvt. Ltd, Ahmedabad, Gujarat 382213, India Specialty Correspondence to: McCall, email: mccallk@ohio.edu Keywords: breast cancer, toll-like receptors,...