A5064670878- S100 Proteins and Annexins
- Plant Pathogens and Resistance
- Agriculture, Plant Science, Crop Management
- Potato Plant Research
- Advanced Glycation End Products research
- Protease and Inhibitor Mechanisms
- Nanoparticle-Based Drug Delivery
- Photodynamic Therapy Research Studies
- Protein Hydrolysis and Bioactive Peptides
- Nanoplatforms for cancer theranostics
- Glycosylation and Glycoproteins Research
- Surfactants and Colloidal Systems
- Carbohydrate Chemistry and Synthesis
- Peptidase Inhibition and Analysis
- Plant Disease Resistance and Genetics
- Cancer Research and Treatments
- Plant Virus Research Studies
- Biochemical effects in animals
- Alcohol Consumption and Health Effects
- Polymer Surface Interaction Studies
- Natural Antidiabetic Agents Studies
- Parathyroid Disorders and Treatments
- Signaling Pathways in Disease
- Protein Interaction Studies and Fluorescence Analysis
- Chronic Kidney Disease and Diabetes
Wroclaw Medical University
2012-2024
University of Wrocław
2010-2020
Niepubliczna Wyższa Szkoła Medyczna
2008
Institute of Plant Genetics, Polish Academy of Sciences
1975
Plant Protection Institute
1975
Glycation is a physiological process that determines the aging of organism, while in states metabolic disorders it significantly intensified. High concentrations compounds such as reducing sugars or reactive aldehydes derived from lipid oxidation, occurring for example diabetes, atherosclerosis, dyslipidemia, obesity syndrome, lead to increased glycation proteins, lipids and nucleic acids. The level advanced end-products (AGEs) body depends on rapidity their production rate removal by...
Abstract Non-enzymatic modification of proteins by carbohydrates, known as glycation, leads to generation advanced glycation end-products (AGEs). In our study we used in vitro generated AGEs model vivo. We discovered vivo analogs unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized under anhydrous conditions with bovine serum albumin and myoglobin. Using nuclear magnetic resonance spectroscopy have identified a set isomers, open-chain cyclic structures, the fructosamine...
Advanced glycation end-products (AGEs) contribute to vascular complications and organ damage in diabetes. The unique AGE epitope (AGE10) has recently been identified human serum using synthetic melibiose-derived (MAGE). We aimed at developing ELISA for AGE10 quantification, determining whether is present diabetic patients (n = 82), evaluating its association with complications. In a competitive developed, the reaction of MAGE anti-MAGE was inhibited by physiological serum. this assay, new...
Enolase, is a glycolytic enzyme ubiquitous in higher organisms, where it forms tissue specific dimers of isoforms, also found the cytoplasm fermentative bacteria. The aim this work was to identify enolase-like proteins cell wall some Gram-negative bacteria using antibodies against human β-enolase, an isoenzyme skeletal and heart muscles. Cell outer membrane protein (OMP) preparations were obtained from 9 strains Enterobacteriaceae one Pseudomonas aeruginosa. Specific enzymatic enolase...
Methylglyoxal (MG) was studied as an inhibitor and effective glycating factor of human muscle-specific enolase. The inhibition carried out by the use a preincubation procedure in absence substrate. Experiments were performed anionic cationic buffers showed that enolase methylglyoxal formation enolase-derived glycation products arose more effectively slight alkaline conditions presence inorganic phosphate. Incubation 15 micromolar solutions enzyme with 2 mM, 3.1 mM 4.34 MG 100 phosphate...
The authors examine properties of daunorubicin (DNR)‐loaded oil‐core multilayer nanocapsules prepared via layer‐by‐layer approach with different polyelectrolyte (PE) coatings such as a standard one (containing polysodium 4‐styrenesulphonate/poly(diallyldimethyl‐ammonium) chloride) and polysaccharide‐based shell (dextran/chitosan), in regard to the outer layer poly‐ l ‐glutamic acid (PGA) grafted polyethylene glycol (PGA‐ g ‐PEG). nanocarriers are obtained on cationic nanoemulsion template...
Matrix metalloproteinase 2 (MMP-2) is activated in hearts upon ischemia-reperfusion (IR) injury and cleaves sarcomeric proteins. It was shown that carvedilol nebivolol reduced the activity of different MMPs. Hence, we hypothesized they could reduce MMPs activation myocytes, therefore, protect against cardiac contractile dysfunction related with IR injury. Isolated rat were subjected to either control aerobic perfusion or injury: 25 min perfusion, followed by 20 global, no-flow ischemia,...
Advanced glycation end-products (AGEs) are capable of stimulating oxidative stress and inflammation. This study investigates the synthesis medium crosslinked AGEs (the most optimal form because soluble in water, used many assays as markers) their biochemical properties.
Enolase detected on the cell surface may be a receptor for certain ligands, especially plasminogen. It is important pathogen invasiveness and in development of tumour. Therefore, we sought to preliminarily determine enolase location catalytic activity subfractions MCF-7 cells. The latter was done intact cells We identified by immunoblotting. binding human plasminogen performed immunoblotting using monoclonal antibodies against demonstrated enolase. postnuclear perinuclear fractions...
Pseudomonas aeruginosa is one of the pathogenic bacteria which utilize binding host plasminogen (Plg) to promote their invasion throughout tissues. In present study, we confirmed that P. exhibits affinity for human plasminogen. Furthermore, showed protein detected on cell wall and an enolase-like protein. The hypothesis alpha-enolase, a cytoplasmatic glycolytic enzyme, resides also surface bacterium was supported by electron microscopy analysis. plasminogen-binding activity bacterial outer...
Enolase - a glycolytic enzyme is also expressed on the surface of eukaryotic cells such as macrophages, neutrophils, endothelial, neuronal, tumor cells. Surface enolase plasminogen receptor plays an important role in myogenesis, tumorgenesis and angiogenesis. Determination localization cell lines may give rise to elucidation its function The cellular muscle-specific isoform normal rat cardiomyocytes (H9c2, embryonic heart-derived line) sarcoma (R1) line reported here. Immunocytochemical...