A5036038478- Nitric Oxide and Endothelin Effects
- Sulfur Compounds in Biology
- Molecular Sensors and Ion Detection
- Redox biology and oxidative stress
- Trypanosoma species research and implications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Electron Spin Resonance Studies
- Hemoglobin structure and function
- Adenosine and Purinergic Signaling
- Synthesis and Biological Evaluation
- Research on Leishmaniasis Studies
- Renin-Angiotensin System Studies
- Electrochemical sensors and biosensors
- Groundwater and Watershed Analysis
- Multicomponent Synthesis of Heterocycles
- Educational Research and Science Teaching
- Botulinum Toxin and Related Neurological Disorders
- Nanoplatforms for cancer theranostics
- bioluminescence and chemiluminescence research
- Human Rights and Immigration
- Microwave-Assisted Synthesis and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Adsorption and biosorption for pollutant removal
- Cardiac Ischemia and Reperfusion
- Photochromic and Fluorescence Chemistry
Universidad de la República
2014-2024
Centro Científico Tecnológico - San Juan
2019
Universidad de Montevideo
2017
National University of Mar del Plata
2017
Consejo Nacional de Investigaciones Científicas y Técnicas
2017
Universidad Nacional de Córdoba
2015
Significance Vascular oxidative stress and endothelial cell dysfunction contribute to various human pathologies. Here, we show that, in vascular cells, a key mitochondrial antioxidant enzyme, namely thioredoxin reductase 2 (TrxR2), plays central role the control of integrity. Its targeted loss is associated with disruption both nitric oxide (·NO) redox homeostasis vivo. Impaired TrxR2 activity leads elevated steady-state levels peroxynitrite, reflecting decreased bioavailability ·NO by its O...
Abstract Demyelinated brain lesions, a hallmark of autoimmune neuroinflammatory diseases like multiple sclerosis, result from oligodendroglial cell damage. Activated microglia are considered major source nitric oxide and subsequent peroxynitrite‐mediated damage myelin. Here, we provide biochemical biophysical evidence that the oxidoreductase glutaredoxin 2 inhibits peroxynitrite formation by transforming into dinitrosyl‐diglutathionyl‐iron‐complexes. Glutaredoxin levels influence both...
Human Mn-containing superoxide dismutase (hMnSOD) is a mitochondrial enzyme that metabolizes radical (O2(•-)). O2(•-) reacts at diffusional rates with nitric oxide to yield potent nitrating species, peroxynitrite anion (ONOO(-)). MnSOD nitrated and inactivated in vivo, active site Tyr34 as the key oxidatively modified residue. We previously reported k of ∼1.0 × 10(5) M(-1) s(-1) for reaction hMnSOD ONOO(-) by direct stopped-flow spectroscopy critical role Mn nitration process. In this study,...
Significance In Chagas disease (CD), macrophages are the first line of defense against its causative agent, Trypanosoma cruzi . Here, we show that superoxide radical (O 2 •− ), a reactive species produced during phagocytosis, diffuses toward T. , causing toxicity. Much O permeation involves protonation inside acidic phagosome. To deal with host-derived oxidants, contains broad antioxidant enzyme armamentarium. Herein, generated parasites overexpressing cytosolic dismutase (Fe-SODB) and...
Cantú syndrome is a multisystem disorder caused by gain-of-function (GOF) mutations in KCNJ8 and ABCC9, the genes encoding pore-forming inward rectifier Kir6.1 regulatory sulfonylurea receptor SUR2B subunits, respectively, of vascular ATP-sensitive K+ channels (KATP). In this study, we investigated changes endothelium mice which -associated Kcnj8 or Abcc9 were knocked-in to endogenous loci. We found that endothelium-dependent dilation was impaired small mesenteric arteries from mice. Loss...
Peroxymonocarbonate (HCO4–/HOOCO2–) is produced by the reversible reaction of CO2/HCO3– with H2O2 (K = 0.33 M–1, pH 7.0). Although in low yields at physiological pHs and concentrations, HCO4– oxidizes most nucleophiles rate constants 10 to 100 times higher than those H2O2. Boronate probes are known examples because reacts coumarin-7-boronic acid pinacolate ester (CBE) a constant that approximately same holds for fluorescein-boronate (Fl-B) as reported here. Therefore, we tested whether...
Reactive oxygen and nitrogen species have been implicated in many biological processes diseases, including immune responses, cardiovascular dysfunction, neurodegeneration, cancer. These chemical are short-lived settings, detecting them these conditions diseases requires the use of molecular probes that form stable, easily detectable, products. The mechanisms limitations currently used not well-understood, hampering their effective applications. Boronates emerged as a class for detection...
Nitro-arachidonic acid (NO2-AA) is a cell signaling nitroalkene that exerts anti-inflammatory activities during macrophage activation. While angiotensin II (ANG II) produces an increase in reactive oxygen species (ROS) production and mitochondrial dysfunction renal tubular cells, little known regarding the potential protective effects of NO2-AA ANG II-mediated kidney injury. As such, this study examines impact on II-induced immortalized proximal tubule line (HK-2 cells). Treatment HK-2 cells...
Background: In this paper, we report the solid-phase synthesis of 33 novel 1,2,5-tri-substituted benzimidazole derivatives and their in vitro activity on cruzipain Trypanosoma cruzi epimastigotes. Results: Seven compounds were potent inhibitors T. growth with IC50 values range 6–16 µM. Applying structure–activity relationships principal component analysis strategies able to determine ring substituent effects physicochemical properties that are important for antichagasic these derivatives, as...
An efficient and rapid microwave-assisted solid-phase method for the synthesis of 5-methyl-1,2-disubstituted benzimidazoles derivatives has been developed. The phosphonium linker, obtained by reaction between polymer-supported triphenylphosphine 4-fluoro-3-nitrobenzyl iodide, underwent aromatic substitution with primary amines, followed one-pot aldehydes in presence SnCl2·2H2O, yielded benzimidazole system under microwave irradiation. final products were released from resin NaOH irradiation...
Nuestro primer curso de Química orgánica, donde se presentan los principales grupos funcionales, sus propiedades fisicoquímicas y síntesis, poseía un contenido estrictamente teórico. Por este motivo, en muchas temáticas, especialmente físicas ácido-base compuestos orgánicos reacciones reconocimientos funcionales estudiantes no conseguían traspasar la barrera del aprendizaje significativo. eso, como forma complementar fortalecer instancia adquisición conocimientos, decidimos implementar una...